Uses of modified mullerian inhibiting substance (mis) proteins for the treatment of neurodegenerative diseases

ABSTRACT

The present invention relates to methods to treat a neurodegenerative disease or disorder, e.g., a motor neuron disease in a subject, whereby the subject is administered a recombinant human Mullerian Inhibiting Substance (MIS) protein as disclosed herein, wherein the recombinant human MIS protein comprises a modified Kex cleavage site for increased cleavage. The recombinant human MIS protein can be produced from a pre-proprotein comprising a non-MIS leader sequence or a functional fragment thereof in place of the MIS leader sequence.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a Continuation of U.S. application Ser. No.15/023,044 filed Mar. 18, 2016, which is a 371 National Phase Entry ofInternational Patent Application No. PCT/US2014/024187 filed on Mar. 12,2014, which claims benefit under 35 U.S.C. § 119(e) of U.S. ProvisionalApplication No. 61/880,451 filed Sep. 20, 2013, and U.S. ProvisionalApplication No. 61/881,719 filed Sep. 24, 2013, the contents of each ofwhich are incorporated herein by reference in their entireties.

GOVERNMENT SUPPORT

This invention was made with government support under grant NumberCA17393 awarded by the National Institutes of Health (NIH). TheGovernment has certain rights in the invention.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has beensubmitted electronically in ASCII format and is hereby incorporated byreference in its entirety. Said ASCII copy, created on Mar. 12, 2014, isnamed 030258-076965-PCT_SL.txt and is 28,165 bytes in size.

FIELD OF THE INVENTION

The present invention relates to modified recombinant human MIS proteinwhich has improved cleavage and increased bioactivity and increasedpotency as compared to wild-type human MIS protein. In some aspects, therecombinant human MIS protein comprises at least one of the following: amodified Kex cleavage site for increased cleavage, a FLAG Tag, and anon-MIS leader sequence in place of the normal MIS leader sequence.Other aspects of the invention relate to methods, uses and kitscomprising a recombinant human MIS protein for the treatment of cancers,such as those that expresses the MIS receptor type II (MISRII) or forthe treatment of a disease characterized by excess androgen.

BACKGROUND OF THE INVENTION

Mullerian Inhibiting Substance (MIS) also known as anti-Mullerianhormone (AMH), is a 140-kDa disulfide-linked homodimer glycoproteinmember of the large transforming growth factor-β (TGFβ) multigene familyof glycoproteins. The proteins in this gene family are all produced asdimeric precursors and undergo posttranslational processing foractivation, requiring cleavage and dissociation to release bioactiveC-terminal fragments. Similarly, the 140 kilodalton (kDa)disulfide-linked homodimer of MIS is proteolytically cleaved to generateits active C-terminal fragments.

MIS, is a reproductive hormone produced in fetal testes, which inhibitsthe development of female secondary sexual structures in males. Beforesexual differentiation, the fetus is bipotential, and the developmentalchoice of male Wolffian ducts (i.e. prostate, vas deferens) over femaleMullerian ducts (i.e. Fallopian tubes, uterus, vagina) in the male iscontrolled in part by MIS.

The human MIS gene is located on chromosome 19, and its expression issexually dimorphic. In males, MIS expression begins at 9 weeks gestationin the fetal testes and continues at high levels until puberty, whenexpression levels fall dramatically. In females, MIS is produced onlypostnatally in granulosa cells from prepuberty through menopause atlevels similar to adult males, after which expression ceases. In malefetuses MIS causes regression of the Mullerian ducts, the precursors tothe Fallopian tubes, uterus, cervix, and upper third of the vagina.

MIS exerts its biologic effect after binding to a heterodimer of type Iand type II single transmembrane spanning serine threonine kinasereceptors, leading to cross phosphorylation of the GS box kinase domainof the type I receptor by the type II receptor. Subsequently, SMAD 1, 5and 8 (but predominantly SMAD 8) are activated and, together with SMAD4, regulate gene transcription. Only one MIS receptor type II (MISRII)gene has been identified in mice, rats, and rabbits, where in humans itsgene localizes to chromosome 12. It is a 65-kDa protein which has beendetected in embryonic and adult Mullerian structures, breast tissue,prostatic tissue, the gonads, motor neurons, and brain. In the fetus,mesoepithelial cells expressing MISRII in the coelomic epitheliumcovering the urogenital ridge migrate into and become part of themesenchymal cells surrounding the Mullerian duct epithelium. Expressionis also detected in the gonads, as wells as in the ovarian coelomicepithelium. Type I MIS receptors have been identified in mammals, withactivin receptor-like kinase (ALK) 2 and 3 being the most likelycandidates, depending upon animal species and the tissue examined.

In addition to its well established role in the regression of Mullerianducts, MIS inhibits the proliferation of various human cancer cell linesin vitro and in vivo. The cell lines showing inhibition were derivedfrom ovarian, cervical, endometrial, prostate, uterine, Fallopian andbreast cancers. Toxicity has not been observed in vivo even when highconcentrations of MIS are maintained systemically in rodents or in humanpatients with tumors secreting MIS for prolonged periods of time. Thesefindings of relatively restricted receptor expression,anti-proliferative activity against cancer cells expressing the MIS RIand RII, and its apparent non-toxicity, taken together, make MIS anideal reagent for use in combination with existing chemotherapeuticdrugs for the treatment of ovarian cancer, which are known to becomeresistant to these conventional agents.

MIS acts through MIS Type II receptor cells to serve as a potent tumorsuppressor of ovarian cancer initiation (Teixeira et al, unpublished).MIS can also target, as a receptor mediated event the stem/progenitorpopulation of the ovarian cancer cell line (Meirelles et al, 2012; Weiet al, 2010). MIS can be used for the treatment of cancers, for example,expressing MISRII. MISRII is expressed in the majority of epithelialovarian cancers (Masiakos et al. 1999; Bakkum-Gamez et al. 2008; Song etal. 2009).

MIS also inhibits growth of a variety of cancers in vitro and in vivo,without obvious toxicity after prolonged therapy in vivo(Pieretti-Vanmarcke et al. 2006b). Epithelial ovarian cancerrecapitulates the original histology of the embryonic Mullerian ductsand its various subtypes (Scully 1977); for example, serouscystadenocarcinoma resembles embryonic Fallopian tube, endometrioidcarcinoma, the endometrium, and mucinous carcinoma, the cervix. Also,MIS acts synergistically or additively with commonly used cancer drugsto control tumor growth (Pieretti-Vanmarcke et al. 2006a).

It has been previously reported that chemotherapeutic agents select forovarian cancer stem cells, which are typically multi-drug resistant,and/or resistant to chemotherapeutics. In particular, there is a growingbody of research reporting that ovarian cancers and cell lines areheterogeneous, with ovarian cancer stem cell populations that areresistant to chemotherapeutic drugs but remain responsive to MIS. MISparticularly targets ovarian cancer side population cells and apopulation of CD44+, CD24+, EpCam+ and E-Cadherin-negative cells thatare ovarian cancer progenitor cells with stem/progenitor characteristicsthat respond poorly to chemotherapeutic agents currently in clinical usefor ovarian cancer (Wei et al, 2010). In particular, MIS has been shownto inhibit ovarian cancer cells both in-vitro and in-vivo and canspecifically target and inhibit the growth of an ovarian cancerprogenitor cell population enriched by the CD44+, CD24+, Ep-CAM+ andE-cadherin-cell surface markers. In order to accommodate clinicaltesting of MIS in ovarian cancer patients, the production of recombinanthuman MIS must be optimized to increase yield and purity.

MIS belongs to the TGF-β superfamily, a class of proteins involved inmany pathologies including cancer. Recombinant TGF-β proteins have beenvery difficult to produce because they require complex maturationprocess involving pre-pro protein cleavage, dimerization, andglycosylation and disulfide bonding for activity. Previous attempts havebeen plagued by low production, limited cleavage, and lack ofhomogeneity, even in mammalian cells. In particular, MIS can only befeasibly produced in mammalian cells, and not E coli or yeast, whereproduction yields are much higher, and industrial scaling morestraightforward. In mammalian cells, yields and homogeneity of theproduct can be significant barriers to industrial scaling and ultimateentry into clinical trials. For example, proteolytic degradation was acontributing factor to the failure of topical TGF-β3 in early clinicaltrials against chemotherapy-induced oral mucositis in patients withlymphomas and solid tumors. Recombinant BMP-2 in a paste form remainsthe only TGF-β family ligand used in the clinic, and is limited to thespecific indication of autologous bone grafting. Progress in thetechnology of production and purification of TGF-β recombinant proteinscould help many candidates to achieve their therapeutic potential in theclinic.

Accordingly, the preparation resulting from purification of native orwild-type MIS is complex and the yield is low. Furthermore, the cleavagenecessary to produce the active fragment of MIS is also inefficient.Human MIS protein is produced from a pre-proprotein, which comprises aleader sequence. The leader sequence (amino acids 1-25 of SEQ ID NO: 1)is cleaved off and the remaining preprotein (often called “holo-humanMIS”) must be post-translationally cleaved to result in a N-terminal andC-terminal domain. These covalently linked N-terminal and C-terminaldomains form a monomer, and two identical monomers (comprising the N-and C-terminal domains) form together to generate a homodimer.Holo-human MIS is cleaved into its N- and C-terminal domains most likelyby means of furin or a related prohormone convertase PC5, expressed inthe gonads. Cleavage occurs primarily at a kex-like site characterizedby R⁻⁴ XXR⁻¹ with a serine in the +1 site, which makes the MIS cleavagesite monobasic. The purified C-terminal domain is the biologicallyactive moiety and cleavage is required for biological activity. Asecondary cleavage site, whose significance is unknown, is observed lessfrequently at residues 229-230 (which corresponds to amino acid residues254-255 of SEQ ID NO:1). Non-cleavable mutants of MIS are notbiologically active and mutations in the human gene that truncate thecarboxy-terminal domain lead to persistent Mullerian duct syndrome. Therole of the amino-terminal domain in vivo may be to assist in proteinfolding and to facilitate delivery of the C-terminal peptide to itsreceptor. In one study (Cate, Pepinsky, et al.) addition of theN-terminal peptide was shown to enhance the biological activity of theC-terminal moiety in vitro, but the mechanism was unclear. The cleavageof recombinant MIS expressed by CHO cells is incomplete, thus cleavagewith an exogenous serine protease such as plasmin is required to enhancebioactivity.

Accordingly, there is a need for a more efficient method to produce highconcentrations of human MIS protein for use as a therapeutic biologicagent.

SUMMARY OF THE INVENTION

The present invention relates to modified recombinant human MIS proteinwhich has improved cleavage and increased bioactivity and increasedpotency as compared to wild-type human MIS protein, where therecombinant human MIS protein comprises a combination of the following:a modified Kex cleavage site for increased cleavage, and a non-MISleader sequence in place of the normal MIS leader sequence, to improvethe yield of bioactive protein with or without an, internal label, orTag to facilitate its purification.

In order to accommodate clinical testing of MIS in patients, e.g., forthe treatment of neurodegenerative diseases, the production ofrecombinant human MIS must be optimized to increase yield and purity.Here, the inventors have demonstrated that the substitution of the MISleader sequence to that of human serum albumin, combined with amodification of the endogenous cleavage site from RAQR/S (SEQ ID NO: 26)to a furin/kex2 RARR/S (SEQ ID NO: 27) consensus site results in highexpression, increased c-terminus cleavage and a reduction in unwantedcryptic internal cleavage products when produced in CHO cells. PurifiedMIS containing these alterations retains its capacity to induceregression of the Mullerian duct in fetal rat embryonic urogenital ridgeassays.

Accordingly, the inventors have demonstrated herein that modificationsto the MIS protein sequence at the activating cleavage site of MISenhances maturation into the active form, and the addition of a leadersequence from albumin, the most highly secreted protein in the blood,results in higher production yield of cleaved active MIS which does notsuffer from unwanted proteolytic degradation. Furthermore, the inventorsnot only demonstrate herein that the modifications in MIS allows forproduction of high levels of this protein, but unexpectedly themodifications increase activating cleavage of MIS while improving thehomogeneity of the product, all of which are useful for translation ofproduction of MIS for clinical use. Importantly, the inventorssuprisingly discovered that the enhanced cleavage of MIS results in amuch greater activity when it was tested for its ability to induceMullerian duct regression ex vivo. These modifications can beincorporated in other technologies such as viral vectors for genetherapy, for example, for the treatment of neurodegenerative diseases.

Accordingly, herein the inventors have engineered changes to the nativehuman MIS amino acid sequence to do a combination of the following: (i)modify the primary cleavage site to increase cleavage and thus increasethe potency and bioactivity of MIS, without insertion of a tag tofacilitate its purification, and (ii) modify the endogenous leadersequence of MIS to increase yield of bioactive protein. Surprisingly,the addition of the leader sequence in combination with a modifiedprimary cleavage site significantly increased both the yield of proteinproduced and the amount of cleavage from the primary cleavage site ofthe recombinant MIS protein. Furthermore, there is an unmet need to havea form of bioactive MIS that is labeled for use in receptor and otherbinding studies that will be very important both for the selection ofpatients for treatment and for addressing molecular mechanisticquestions regarding the interaction of MIS in various receptor bearingtissues. In addition, the labeled ligand will be essential to determineif another receptor or other binding proteins exist in various tissues.Herein, the inventors demonstrate the production of an internallyepitope tagged MIS that retains full bioactivity in the Mullerian ductregression assay. In one embodiment, the tag is a “FLAG” tag because ofthe availability of high quality reagents used for its detection andpurification.

The inventors also demonstrate that substitution of the MIS leadersequence to that of human serum albumin (HSA), combined with amodification of the primary endogenous cleavage site from RAQR/S (SEQ IDNO: 26) to RARR/S (SEQ ID NO: 27) results in greater expression,increased c-terminus cleavage and a reduction in unwanted crypticinternal cleavage when produced in CHO cells. Purified MIS containingthese alterations retains its capacity to induce regression of theMullerian duct in fetal rat embryonic urogenital ridge assays, and showsincreased potency.

In another embodiment, the recombinant human MIS is engineered with amore efficient cleavage site at the carboxy-terminal end of theN-terminal domain, thereby eliminating the need for exogenous cleavage.This recombinant MIS protein can be used both as a therapeutic and as aprobing molecule, without a tag for identification.

Importantly, the change in the endogenous leader sequence with anotherleader sequence, e.g., a human serum albumin (HSA) leader sequenceincreased production of the MIS protein. Surprisingly, the inventorsdemonstrate that the combination of the leader sequence and modifiedcleavage site increases cleavage from the primary cleavage site from 37%to over 80% which was unexpected, as an increase in protein yield isnormally associated with decreased post-translational processing,including cleavage, because increased protein production typicallysaturates the available or endogeneous cleavage enzymes.

In some embodiments, the recombinant human MIS protein (e.g., thepolypeptide and/or the nucleic acid encoding a recombinant human MISprotein) as disclosed herein, or a functional fragment or derivative orvariant thereof, can be used to treat a neurodegenerative disease, suchas a motor neuron degenerative disease such as amyotrophic lateralsclerosis (ALS), spinal muscular atrophy (SMA), Primary lateralsclerosis (PLS) and other motor neuron degenerative diseases.

Accordingly, one aspect of the present invention relates to arecombinant Mullerian Inhibiting Substance (MIS) protein comprising acombination of a non-MIS leader sequence or a functional fragmentthereof in place of the MIS leader sequence of amino acids 1-25 of SEQID NO: 1, and a modification of at least one amino acid between residues448-452 of SEQ ID NO: 1 to increase cleavage as compared to in theabsence of a modification, wherein the recombinant MIS protein hasincreased cleavage and increased yield of production in vitro ascompared to wild-type MIS protein corresponding to amino acid residuesof SEQ ID NO: 1. In some embodiments, the recombinant MIS protein lacksa leader sequence. In these embodiments, the recombinant MIS protein canbe produced from a pre-proprotein comprising a non-MIS leader sequenceor a functional fragment thereof in place of the MIS leader sequence ofamino acids 1-25 of SEQ ID NO: 1, wherein the leader sequence is cleavedoff during production. In some embodiments, the recombinant MIS proteinfurther comprises a Tag protein.

In some embodiments, a non-MIS leader sequence is an albumin leadersequence or a functional fragment thereof, for example, a human serumalbumin (HSA) leader sequence or a fragment thereof. In someembodiments, the HSA leader sequence comprises the amino acid sequenceof SEQ ID NO: 6 or a variant that is at least 80% homologous thereto, ora functional fragment, e.g., a fragment of the HSA sequence comprisingat least 10 amino acids, or at least about 11, or at least 15 aminoacids of SEQ ID NO: 6 or a variant that is at least 80% homologousthereto. In some embodiments, a fragment of the HSA leader sequence isselected from the group consisting of: MKWVTFISLLFLFSSAYS (SEQ ID NO:13); MKWVTFISLLFLFSSAYSRGVFRR (SEQ ID NO: 6); MKWVSFISLLFLFSSAYS (SEQ IDNO:14).

In some embodiments, a non-MIS leader sequence is selected from a groupconsisting of: immunoglobulin signal peptide fused to a tissue-typeplasminogen activator propeptide (IgSP-tPA), murine immunoglobulinsignal peptide (IgSP), a MPIF-1 signal sequence (MKVSVAALSCLMLVTALGSQA(SEQ ID NO: 15); a stanniocalcin signal sequence (MLQNSAVLLLLVISASA (SEQID NO:16); an invertase signal sequence (MLLQAFLFLLAGFAAKISA (SEQ IDNO:17); a yeast mating factor alpha signal sequence (K. lactis killertoxin leader sequence); a hybrid signal sequence(MKWVSFISLLFLFSSAYSRSLEKR (SEQ ID NO:18)); a HSA/MFα-1 hybrid signalsequence (MKWVSFISLLFLFSSAYSRSLDKR (SEQ ID NO:19)); a K. lactiskiller/MFα-1 fusion leader sequence (MNIFYIFLFLLSFVQGSLDKR (SEQ IDNO:20)); an immunoglobulin Ig signal sequence (MGWSCIILFLVATATGVHS (SEQID NO:21)); a Fibulin B precursor signal sequence(MERAAPSRRVPLPLLLLGGLALLAAGVDA (SEQ ID NO:22)); a clusterin precursorsignal sequence (MMKTLLLFVGLLLTWESGQVLG (SEQ ID NO: 23)); and theinsulin-like growth factor-binding protein 4 signal sequence(MLPLCLVAALLLAAGPGPSLG (SEQ ID NO:24)) or a functional fragment thereof.

In some embodiments, a modification of amino acid 450 of SEQ ID NO: 1from Q to R increases the cleavage from the primary cleavage site in MISas compared to the amount of cleavage in the absence of such amodification. In some embodiments, a recombinant MIS further comprises amodification of amino acid 452 of SEQ ID NO: 1 from S to R to increasecleavage as compared to in the absence of such a modification.

In some embodiments, the recombinant MIS protein disclosed hereincomprises a tag which is a FLAG tag, for example, amino acid sequenceDYKDDDDK (SEQ ID NO: 8), or a functional derivative or variant thereof.In some embodiments, a tag, e.g., FLAG tag is located after amino acidresidue 452 of SEQ ID NO: 1 and before amino acid residue 453 of SEQ IDNO: 1. In some embodiments, the location of the tag, e.g., Flag Tag isbetween amino acid residue 452 and 453 of SEQ ID NO: 1. In someembodiments, the tag is located at the N-terminus of the C-terminaldomain of MIS. In some embodiments, the tag is no longer than 50 aminoacids, for example, no longer than about 50, or about 40, or about 30,or about 20, or about 10 amino acids in length or about 7 amino acids inlength.

In some embodiments, a recombinant MIS protein described hereincomprises the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 3 or afunctional fragment thereof, which can be encoded by nucleic acidsequences SEQ ID NO: 4 and 5 respectively.

Another aspect of the present invention relates to a pharmaceuticalcomposition comprising a recombinant MIS protein as discussed herein anda pharmaceutically acceptable carrier.

Another aspect of the present invention relates to a polynucleotideencoding the recombinant MIS protein as discussed herein, e.g., wherethe polynucleotide corresponds to SEQ ID NO: 4 or SEQ ID NO: 5 or anucleotide which has at least 95% sequence identity to the nucleic acidsequence of SEQ ID NO: 4 or SEQ ID NO: 5 respectively. Another aspect ofthe technology described herein relates to a vector comprising thepolynucleotide of SEQ ID NO: 4 or SEQ ID NO: 5 or a nucleotide which hasat least 95% sequence identity to the nucleic acid sequence of SEQ IDNO: 4 or SEQ ID NO: 5 respectively. In some embodiments, a vector is aviral vector or an expression vector, e.g., pcDNA 3.1, or alternativevectors for E. Coli or bacteriophage. In some embodiments, a viralvector is selected from the group consisting of an adenoviral vector, apoxvirus vector and a lentiviral vector. In some embodiments, a viralvector is adeno-associated virus (AAV), for example, recombinant AAVserotype 9 (rAAV9) as disclosed herein.

In some embodiments, a vector comprises a nucleic acid sequence whichencodes a recombinant MIS protein or fragment thereof which has at least95% sequence identity to the nucleic acid sequence of SEQ ID NO: 4 orSEQ ID NO: 5, and where the nucleic acid sequence is operatively linkedto tissue- or cell-type specific promoter. In some embodiments, a hostcell comprising such a vector is also encompassed in the presentinvention.

In some embodiments, the vector comprising the polynucleotides asdiscussed herein can express the recombinant MIS protein at a constantlevel over a desired period of time.

Another aspect of the present invention relates to a human MIS proteinproduced by post-translational processing of the recombinant human MISprotein as discussed herein.

Another aspect of the technology discussed herein relates to apharmaceutical composition comprising the vector as discussed herein anda pharmaceutically acceptable carrier. Another aspect of the technologydiscussed herein relates a purified preparation, or substantiallypurified human MIS protein produced from the recombinant human MISprotein as discussed herein.

In some embodiments, the recombinant human MIS protein (e.g., thepolypeptide and/or the nucleic acid encoding a recombinant human MISprotein) as disclosed herein, or a functional fragment or derivative orvariant thereof, can be used to treat a neurodegenerative disease, suchas a motor neuron degenerative disease such as amyotrophic lateralsclerosis (ALS), spinal muscular atrophy (SMA), Primary lateralsclerosis (PLS) and other motor neuron degenerative diseases.

Other aspects of the technology as disclosed herein relates to a methodto treat a neurodegenerative disease, such as a motor neurondegenerative disease such as amyotrophic lateral sclerosis (ALS), spinalmuscular atrophy (SMA), Primary lateral sclerosis (PLS) in a subject,the method comprising administering an effective amount of a recombinantMIS protein, wherein the recombinant MIS protein comprises amodification of amino acid 450 of SEQ ID NO: 1 from Q to R, where therecombinant MIS optionally comprises a tag, and wherein the recombinantMIS protein results in at least one of the following; increases themotor neuron survival, prevents or decreases the rate of motor neurondegeneration, prevents or reduces the decrease in muscle strength,promotes muscle strength, decreases or prevents the activation ofastrocytes and/or microglia in the spinal cord in the subject. In someembodiments, the recombinant MIS protein is produced from apre-proprotein comprising a non-MIS leader sequence or a functionalfragment thereof in place of the MIS leader sequence of amino acids 1-25of SEQ ID NO: 1.

In some embodiments, the recombinant MIS protein comprises the aminoacid residues 25-559 of SEQ ID NO: 2 or a functional fragment thereof.

In some embodiments, the recombinant MIS protein comprises the aminoacid residues 25-567 of SEQ ID NO: 3 or a functional fragment thereof.

In some embodiments, a recombinant MIS can be administered by any route,e.g., via intravenous, intradermal, intramuscular, intraarterial,intralesional, percutaneous, or subcutaneous, or by aerosoladministration. In some embodiments, administration is therapeutic orprophylactic administration. In all aspects as discussed herein, asubject is a mammal, e.g., a human. In some embodiments, a viral vector,such as, for example but not limited to AAV, comprising a nucleic acidencoding the recombinant MIS as disclosed herein can be administered byany of the routes described above.

In some embodiments, at least one additional agent is administered tothe subject in combination with (e.g., before, during or after)administration of the recombinant human MIS, such as a therapeuticagent.

Another aspect of the present invention relates to an article ofmanufacture comprising packaging material and a pharmaceuticalcomposition comprising the recombinant MIS protein as discussed herein,wherein the packaging material comprises a label which indicates thepharmaceutical composition may be administered, for a sufficient term atan effective dose, for treating or reducing the risk of aneurodegenerative disease by targeting cells that express a MullerianInhibiting Substance (MIS) receptor.

Other aspects of the technology as disclosed herein relates to a methodof treating a subject affected with a neurodegenerative disease, forwhich the clinician directs the subject to be treated withpharmaceutical composition comprising a recombinant MIS protein asdisclosed herein.

Other aspects of the technology as disclosed herein relates to a kitcomprising a recombinant MIS protein as discussed herein, or preparationof a MIS protein produced by the post-translational processing of arecombinant MIS protein discussed herein, and a pharmaceuticallyacceptable carrier. In some embodiments, the kit comprises a viralvector which expresses a recombinant MIS protein as discussed herein. Insome embodiments, the viral vector is AAV and expresses recombinant MISprotein as discussed herein. In some embodiments, a kit can optionallycomprise instructions of use of the recombinant MIS protein for thetreatment of a neurodegenerative disease, e.g., a motor neuron disease.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1B are schematic drawings showing the design of new recombinantMIS constructs with the albumin leader sequence. FIG. 1A shows theleader sequence of MIS (25 amino acids) and albumin (24 amino acids)have 20% identity and 5 conserved amino acids. FIG. 1B is a schematicdrawing showing the design of the RF (modified cleavage site plus Flagtag), LRF (leader sequence plus modified cleavage site plus Flag tag),and LR (leader sequence plus modified cleavage site) constructsincluding the placement of the flag tag (F), the modified cleavage site(R), and the albumin leader (L).

FIGS. 2A-2B show MIS production and cleavage in CHOK1 clones stablytransfected with recombinant human LR-MIS and LRF-MIS constructs inconditioned media and screening by western blot. Western blot of 4%reduced SDS gels of media supernatant after 72 hours in culture using ananti-MIS goat polyclonal antibody targeting the c-terminus of MIS(1:200). Purified RF-MIS, CHO93 media and B9 media shown as positivecontrols. MIS production and cleavage in CHOK1 clones stably transfectedwith LR-MIS and LRF-MIS constructs. FIG. 2A shows a western blot ofmedia (10% serum) supernatant after 72 h in culture using an anti-MISgoat polyclonal antibody targeting the holo and N-terminus of MIS(1:200). FIG. 2B shows a western blot of media supernatant after 24 h inculture in serum-free media, using an anti-MIS goat polyclonal antibodytargeting the C-terminus of MIS (1:200). Purified RF-MIS is shown as apositive control.

FIGS. 3A-3C show purified recombinant MIS analyzed by western blot ofreduced SDS gels to estimate the amount of cleavage. Purified WT-MIS,LR-MIS and LRF-MIS and RF-MIS is compared: FIG. 3A shows a western blotof 0.1 μg of each of purified recombinant RF-MIS, LRF-MIS and WT-MIS iscompared using an antibody against the N-terminus (MGH 4) which canrecognize holoenzyme MIS monomer, the cleaved N-terminus, and crypticcleavage products containing part of the N-terminus. FIG. 3B shows awestern blot of 0.1 μg of each of purified recombinant RF-MIS, LRF-MISand WT-MIS using an antibody against the C-terminus which can recognizeholo MIS monomer, the cleaved C-terminus, and cryptic cleavage productscontaining part of the C-terminus. FIG. 3C shows staining apolyacrilamide gel after electrophoresis with 1 μg of each type of MISwith a non-specific protein stain (Lumitein™).

FIGS. 4A-4C show the comparison of 1, 3 and 5 ug/ml (35 uM) of WT-MIS,RF-MIS, LRF-MIS and LR-MIS recombinant MIS variants in a Mullerian ductregression bioassay. Recombinant human MIS produces was incubated for 72h with fetal rat uro-genital ridges. FIG. 4A shows representativesections from both the treated ridge and the untreated contralateralcontrol ridge are compared for Mullerian duct regression, and the scoresare indicated at the bottom left corner of each picture, with 5 beingfull regression and 0 no regression. FIG. 4B shows dilutions ofrecombinant LR-MIS in a Mullerian duct regression bioassay. MIS wasincubated 72 h with fetal rat urogenital ridges at concentrations of 0.2μg/ml and 0.5 μg/ml. The bioassay score is displayed on the bottom leftof each panel with 5 being full regression and 0 no regression. FIG. 4Cis a histogram showing the frequency distribution of those scores inFIG. 4A. (LRF-MIS N=6, RF-MIS N=39). W, Wolffian duct; M, Mullerianduct. Microscopy pictures were taken with a 200× objective.

FIGS. 5A-5B show the amino acid of wild-type MIS protein (SEQ ID NO: 1)with the corresponding amino acid residues using conventionalnomenclature of amino acid labeling (where the first numbered amino acidbegins after the leader sequence). FIG. 5A shows the amino acid sequenceof wild-type MIS protein of SEQ ID NO: 1, showing the leader sequence(in bold) and the primary and secondary cleavage sites highlighted. Thecorresponding amino acid numbering using conventional numbering is shownin brackets. FIG. 5B shows a Table indicating features on amino acidresidues on SEQ ID NO: 1 which correspond with the amino acid residuesusing normal nomenclature of MIS (where the first numbered amino acidbegins after the leader sequence). FIG. 5B discloses “RAQR/S” as SEQ IDNO: 26.

FIG. 6 shows the effect of AAV9 expressing MIS protein on the survivalof a mouse model of ALS. The Kaplan-Meyer survival curves of ALS miceintravenously administeredAAV9-mMIS at post natal day 1 (P1) as comparedto control treated ALS mice demonstrate that rAAV9-mMIS mice have a 19day increase in the average lifespan (approximately 12% increase inlifespan) as compared to control treated ALS mice injected with PBS. N=4for PBS treated controls, n=9 for AAV-mMIS treated ALS mice. The age for50% survival for AAV-mMIS is 172 days as compared to 153 days for PBScontrol treated ALS mice (p=0.013). Data from injection of rAAV9-mMIS onP7 and P28 was also significant (data not shown).

FIGS. 7A-7C show expression of AAV9-mMIS in ALS mouse brain, liver andspinal cord of ALS mice. FIG. 7A shows detection of mMIS expression byRT-PCR (normalized to beta-actin loading control) showing expression ofMIS in the liver and brain of ALS mice after i.v. administration ofAAV9-mMIS at P1 and P7 days old. FIG. 7B shows detection of mMISexpression by western blot after intravenous administration ofrAAV9-mMIS in various tissues at ALS disease endpoint. Mouse MIS proteinwas detected in the muscle (M), liver (L), brain (B) and spinal cord(SC) of ALS mice intravenously administered rAAV9-mMIS, demonstratingthat the AAV9 crosses the blood brain barrier. FIG. 7C shows resultsfrom a q-PCR assay for the amount of viral genome in the brain and liverof rAAV9-mMIS treated mice administered rAAV-mMIS at age P28. Eachsymbol represents one mouse.

FIGS. 8A-8D show immunostaining of the lumbar ventral horn of the spinalcord of rAAV9-mMIS treated ALS mice. FIG. 8A shows immunostaining ofSMI-31 motor neuron marker of PBS control treated ALS mice, FIG. 8Bshows immunostaining of the motor neuron marker of SMI-31 of rAAV9-mMIStreated ALS mice, demonstrating the increase in number of survivingmotor neurons (and neuronal protection) as compared to the controltreated mice shown in FIG. 8A. FIG. 8C shows immunostaining withanti-MIS antibody of the spinal cord of rAAV9-mMIS treated ALS mice, andFIG. 8D shows the merged image of the immunostaining with the motorneuron marker of SMI-31 and an anti-MIS antibody, showingco-localization of MIS and SMI-31 demonstrating that mMIS expressed fromthe AAV9 is expressed in surviving motor neurons.

FIGS. 9A-9B show astroglia and microglia immunostaining of the ventralhorn of the spinal cord of rAAV9-mMIS treated ALS mice. FIG. 9A showsimmunostaining with an astrocyte marker GFAP in the spinal cord of PBScontrol treated ALS mice (left panel) or rAAV9-mMIS treated ALS mice(middle and right panel) demonstrating significantly decreasedexpression of GFAP and thus less astrocyte activation in the spinal cordof rAAV9-mMIS treated ALS mice. FIG. 9B is a histogram showing thefraction of the spinal cord with activated astrocytes in PBS andrAAV9-mMIS treated ALS mice, as compared to normal wild-type (non-ALS)mice, demonstrating that intravenous administration of rAAV9-mMISdecreases the activated astrocytes to a level which is close to that ofwild-type mice.

FIGS. 10A-10B show rAAV vector genomes expressing WT-MIS or modifiedMIS. FIG. 10A shows a shematic of a construct of native mouse MIS andFIG. 10B shows an AAV9 construct of mutated mouse MIS with its cleavagesite ablated as a negative control vector.

FIGS. 11A-11C show rAAV vector genomes expressing 3 different modifiedhMIS variants. FIG. 11A shows a schematic of an AAV9 constructexpressing LRF: rAAV expressing human MIS with a modified cleavage site,a leader peptide and Flag tag added. FIG. 11B shows a schematic of aAAV9 construct expressing LR: rAAV expressing human MIS, with a modifiedcleavage site and a leader peptide added. FIG. 11C shows a schematic ofan AAV9 construct expressing RF: rAAV expressing human MIS with amodified cleavage site and a flag tag.

FIG. 12 shows expression of MIS in serum of blood in vivo. MISconcentration, as determined by ELISA was present in the serum of nudemice injected at 5-6 weeks of age with 1×10¹¹ AAV9-hMIS carrying LFR, LRand RF. AAV9-LR-hMIS resulted in highest amount of MIS in the serum invivo.

FIG. 13 shows a reduction of tumor volume of OVCAR5 xenograft cells innude mice treated with AAV9-LR-hMIS in vivo. At 3 weeks of age, 1million human ovarian cancer cells from OVCAR5 cell line werexenografted subcutaneously in the flank of nude mice injected at 5-6weeks of age with 1×10¹¹ AAV9-hMIS carrying LR. Tumor growth wassignificantly inhibited in AAV9-LR-hMIS treated mice as compared toAAV9-GFP treated controls.

FIG. 14 shows immunofluorescence for GFP in the muscle of AAV9-GFPtreated mice. The GFP vector demonstrated fluorescence in the musclecell wall and liver (data not shown).

FIGS. 15A-15B show rAAV vector genomes expressing 2 different modifiedLR-hMIS variants. FIG. 15A shows a schematic of an AAV9 constructexpressing LR; with human MIS with a modified cleavage site and a leaderpeptide added and FIG. 15B shows mutated human MIS with leader peptideadded, but cleavage site mutated.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to modified recombinant human MIS proteinwhich has at least one of the following characteristics; improvedcleavage, increased bioactivity, increased potency and can be producedat high yield as compared to the wild-type human MIS protein, where therecombinant human MIS protein comprises a combination of the following:a modified Kex cleavage site for increased cleavage and a non-MIS leadersequence in place of the normal MIS leader sequence, to improve theyield of bioactive protein. In some embodiments, this modified MIS iswith or without an internal label, or Tag, to facilitate itspurification.

Accordingly, herein the inventors have engineered changes to the nativehuman sequence to increase endogenous cleavage and thus the potency ofMIS. The inventors have also, optionally, inserted a tag to facilitateits purification.

The inventors have also additionally modified recombinant human MISprotein to comprise a non-MIS leader sequence instead of the 25 aminoacid MIS leader sequence of amino acids 1-25 of SEQ ID NO:1. In someembodiments, the leader sequence comprises an albumin leader sequence,such as a human serum albumin sequence (HSA) or a functional fragment orvariant thereof. In some embodiments, the leader sequence comprises 24amino acids of SEQ ID NO: 6 or a functional fragment thereof, andreplaces amino acid residues 1-25 of SEQ ID NO: 1. This addition,surprisingly, has further increased cleavage of the recombinant MISprotein. This combination has led to higher yield of a product that ismore homogeneous, with increased potency due to increased cleavage. Thiscombination of changes yields a recombinant human MIS variant that canmeet a previously unmet need to have a form of bioactive MIS that islabeled for use in receptor and other binding studies that will be veryimportant both for the selection of patients for treatment and foraddressing molecular mechanistic questions regarding the interaction ofMIS in various receptor bearing tissues. In addition, the labeled ligandwill be essential to determine if another receptor or other bindingproteins exist in various tissues. Herein, the inventors demonstrate theproduction of an internally epitope tagged MIS that retains fullbioactivity in the Mullerian duct regression assay. In one embodiment,the tag is a “FLAG” tag because of the availability of high qualityreagents used for its detection and purification.

As discussed herein, the present invention provides a method fortreating a variety of conditions by administering an effective amount ofa recombinant human MIS protein and functional fragments and derivativesthereof as disclosed herein to a subject in need thereof. Conditionsthat may be treated by the compounds of this invention, or apharmaceutical composition containing the same, include any conditionwhich is treated or reduces the symptoms by administration of human MISor activation of MIS signaling or activation of MISRII, and therebybenefit from administration of a a recombinant human MIS protein andfunctional fragments and derivatives thereof. Representative conditionsin this regard include, for example, but not limited to,neurodegenerative diseases, cancers that express MIS receptors, forexample cancer that express MISRII, for example, but not limited toovarian, cervical and endometrial cancer. Other conditions which can betreated with MIS or activation of MIS signalling reduces the symptomsare proliferative diseases such as cancer, or abnormally high androgenstages such as polycysic ovarian disease, precocious puberty, and otherhyperandrogen disorders, such as testotoxicosis, or anyandrogen-dependent tumor such as prostate cancer.

Definitions

For convenience, certain terms employed in the entire application(including the specification, examples, and appended claims) arecollected here. Unless defined otherwise, all technical and scientificterms used herein have the same meaning as commonly understood by one ofordinary skill in the art to which this invention belongs.

The term “Mullerian Inhibiting Substance” and “MIS” are usedinterchangeably herein and is also known as anti-Mullerian hormone orAMH, refer to compounds and materials which are structurally similar toMIS. By “MIS” or “Mullerian Inhibiting Substance” is meant a polypeptidehaving an amino acid sequence at least about 60%, or at least about 70%,or at least about 80%, or at least about 90%, or at least about 95%, orat least about 96%, or at least about 97%, or at least about 98%, or atleast about 99% identical to amino acid residues 26-560 of SEQ ID NO: 1.The present invention is intended to include mutant forms of recombinanthuman MIS which have substantially the same, or greater biologicalactivity as wild-type MIS. Examples of such mutant MIS moleculescarrying a deletion, insertion, or alteration in the amino acid sequenceof wild-type MIS (e.g., amino acid residues 26-560 of SEQ ID NO:1).Other forms of include substances are for example, salts, functionalderivatives and aglycone forms of wild-type MIS and recombinant humanMIS. Additionally, human recombinant MIS protein can be obtained usingrecombinant DNA technology, or from chemical synthesis of the MISprotein. For reference purposes only, the wild-type human MIS nucleicacid corresponds to Ref Seq No: NM_000479, which are incorporated hereinby reference.

The term “Mullerian Inhibiting Substance type II receptor” or “MISRII”are used interchangeably herein refer to the type II receptor for MIS.The term MISRII is intended to encompass all MIS receptors substantiallyhomologous to MISRII and functional derivatives of MISRII. MISRII isalso known by the alias as AMHR2, and for reference purposes, thenucleic acid sequence of human MISRII corresponds to NM_020547 andGenBank No: AF172932 which are incorporated herein by reference

The term “wild type” refers to the naturally-occurring polynucleotidesequence encoding a protein, or a portion thereof, or protein sequence,or portion thereof, respectively, as it normally exists in vivo.Accordingly, as disclosed herein, the wild type amino acid sequence forthe pre-proprotein of human MIS corresponds to SEQ ID NO: 1, where aminoacid residues 1-25 correspond to the leader sequence. The proprotein ofMIS comprises amino acid residues 26-560 of SEQ ID NO: 1 (e.g., lackingthe 1-25 leader sequence), which is then post-translationally processedby cleavage as discussed herein to form a bioactive MIS homodimer.

The term “soluble MIS polypeptide” as used herein refers to a MISpolypeptide that does not comprise at least part of, or all of, theamino acids which allow it to functionally bind to the membrane.

By a “polynucleotide encoding MIS” is meant a polynucleotide encoding apolypeptide having at least about 60%, or at least about 70%, or atleast about 80%, or at least about 90%, or at least about 95%, or atleast about 96%, or at least about 97%, or at least about 98%, or atleast about 99% sequence identity to any of the amino acid sequencescorresponding to amino acid residues 26-560 of SEQ ID NO: 1.

The term “mutant” refers to any change in the genetic material of anorganism, in particular a change (i.e., deletion, substitution,addition, or alteration) in a wild-type polynucleotide sequence or anychange in a wild-type protein sequence. The term “variant” is usedinterchangeably with “mutant”. Although it is often assumed that achange in the genetic material results in a change of the function ofthe protein, the terms “mutant” and “variant” refer to a change in thesequence of a wild-type protein regardless of whether that change altersthe function of the protein (e.g., increases, decreases, imparts a newfunction), or whether that change has no effect on the function of theprotein (e.g., the mutation or variation is silent). The term mutationis used interchangeably herein with polymorphism in this application.

The term “agent” or “compound” as used herein refers to a chemicalentity or biological product, or combination of chemical entities orbiological products, administered to a subject to treat or prevent orcontrol a disease or condition. The chemical entity or biologicalproduct is preferably, but not necessarily a low molecular weightcompound, but may also be a larger compound, or any organic or inorganicmolecule, including modified and unmodified nucleic acids such asantisense nucleic acids, RNAi, such as siRNA or shRNA, peptides,peptidomimetics, receptors, ligands, and antibodies, aptamers,polypeptides, nucleic acid analogues or variants thereof. For example,an oligomer of nucleic acids, amino acids, or carbohydrates includingwithout limitation proteins, oligonucleotides, ribozymes, DNAzymes,glycoproteins, siRNAs, lipoproteins, aptamers, and modifications andcombinations thereof.

The term “nucleic acid” is well known in the art. A “nucleic acid” asused herein will generally refer to a molecule (i.e., strand) of DNA,RNA or a derivative or analog thereof, comprising a nucleobase. Anucleobase includes, for example, a naturally occurring purine orpyrimidine base found in DNA (e.g. an adenine “A,” a guanine “G.” athymine “T” or a cytosine “C”) or RNA (e.g. an A, a G. an uracil “U” ora C). The term “nucleic acid” encompasses the terms “oligonucleotide”and “polynucleotide,” each as a subgenus of the term “nucleic acid.” Theterm “oligonucleotide” refers to a molecule of between about 3 and about100 nucleobases in length. The term “polynucleotide” refers to at leastone molecule of greater than about 100 nucleobases in length. The term“nucleic acid” also refers to polynucleotides such as deoxyribonucleicacid (DNA), and, where appropriate, ribonucleic acid (RNA). The termshould also be understood to include, as equivalents, analogs of eitherRNA or DNA made from nucleotide analogs, and, as applicable to theembodiment being described, single (sense or antisense) anddouble-stranded polynucleotides. The terms “polynucleotide sequence” and“nucleotide sequence” are also used interchangeably herein.

As used herein, the term “gene” refers to a nucleic acid comprising anopen reading frame encoding a polypeptide, including both exon and(optionally) intron sequences. A “gene” refers to coding sequence of agene product, as well as non-coding regions of the gene product,including 5′UTR and 3′UTR regions, introns and the promoter of the geneproduct. These definitions generally refer to a single-strandedmolecule, but in specific embodiments will also encompass an additionalstrand that is partially, substantially or fully complementary to thesingle-stranded molecule. Thus, a nucleic acid may encompass adouble-stranded molecule or a double-stranded molecule that comprisesone or more complementary strand(s) or “complement(s)” of a particularsequence comprising a molecule. As used herein, a single strandednucleic acid may be denoted by the prefix “ss”, a double strandednucleic acid by the prefix “ds”, and a triple stranded nucleic acid bythe prefix “is.” The term “gene” refers to the segment of DNA involvedin producing a polypeptide chain, it includes regions preceding andfollowing the coding region as well as intervening sequences (introns)between individual coding segments (exons). A “promoter” is a region ofa nucleic acid sequence at which initiation and rate of transcriptionare controlled. It may contain elements at which regulatory proteins andmolecules may bind, such as RNA polymerase and other transcriptionfactors, to initiate the specific transcription of a nucleic acidsequence. The term “enhancer” refers to a cis-acting regulatory sequenceinvolved in the transcriptional activation of a nucleic acid sequence.An enhancer can function in either orientation and may be upstream ordownstream of the promoter.

As used herein, the term “gene product(s)” is used to refer to includeRNA transcribed from a gene (e.g., mRNA), or a polypeptide encoded by agene or translated from RNA.

The terms “polypeptide” and “protein” are used interchangeably to referto a polymer of amino acid residues, and are not limited to a minimumlength. Peptides, oligopeptides, dimers, multimers, and the like, arealso composed of linearly arranged amino acids linked by peptide bonds,and whether produced biologically, recombinantly, or synthetically andwhether composed of naturally occurring or non-naturally occurring aminoacids, are included within this definition. Both full-length proteinsand fragments thereof are encompassed by the definition. The terms alsoinclude co-translational (e.g., leader sequence cleavage of amino acids1-25 of SEQ ID NO:1) and post-translational modifications of thepolypeptide, such as, for example, disulfide-bond formation,glycosylation, acetylation, phosphorylation, proteolytic cleavage (e.g.,cleavage by furins or metalloproteases and prohormone convertases(PCs)), and the like. Furthermore, for purposes of the presentinvention, a “polypeptide” encompasses a protein that includesmodifications, such as deletions, additions, and substitutions(generally conservative in nature as would be known to a person in theart), to the native sequence, as long as the protein maintains thedesired activity. These modifications can be deliberate, as throughsite-directed mutagenesis, or can be accidental, such as throughmutations of hosts that produce the proteins, or errors due to PCRamplification or other recombinant DNA methods. Polypeptides or proteinsare composed of linearly arranged amino acids linked by peptide bonds,but in contrast to peptides, has a well-defined conformation. Proteins,as opposed to peptides, generally consist of chains of 50 or more aminoacids. For the purposes of the present invention, the term “peptide” asused herein typically refers to a sequence of amino acids of made up ofa single chain of D- or L-amino acids or a mixture of D- and L-aminoacids joined by peptide bonds. Generally, peptides contain at least twoamino acid residues and are less than about 50 amino acids in length.

The incorporation of non-natural amino acids, including syntheticnon-native amino acids, substituted amino acids, or one or more D-aminoacids into the peptides (or other components of the composition, withexception for protease recognition sequences) is desirable in certainsituations. D-amino acid-containing peptides exhibit increased stabilityin vitro or in vivo compared to L-amino acid-containing forms. Thus, theconstruction of peptides incorporating D-amino acids can be particularlyuseful when greater in vivo or intracellular stability is desired orrequired. More specifically, D-peptides are resistant to endogenouspeptidases and proteases, thereby providing better oral trans-epithelialand transdermal delivery of linked drugs and conjugates, improvedbioavailability of membrane-permanent complexes (see below for furtherdiscussion), and prolonged intravascular and interstitial lifetimes whensuch properties are desirable. The use of D-isomer peptides can alsoenhance transdermal and oral trans-epithelial delivery of linked drugsand other cargo molecules. Additionally, D-peptides cannot be processedefficiently for major histocompatibility complex class II-restrictedpresentation to T helper cells, and are therefore less likely to inducehumoral immune responses in the whole organism. Peptide conjugates cantherefore be constructed using, for example, D-isomer forms of cellpenetrating peptide sequences, L-isomer forms of cleavage sites, andD-isomer forms of therapeutic peptides. In some embodiments, arecombinant human MIS protein is comprised of D- or L-amino acidresidues, as use of naturally occurring L-amino acid residues has theadvantage that any break-down products should be relatively non-toxic tothe cell or organism.

In yet a further embodiment, a recombinant human MIS protein orfragments or derivatives thereof can be a retro-inverso peptides. A“retro-inverso peptide” refers to a peptide with a reversal of thedirection of the peptide bond on at least one position, i.e., a reversalof the amino- and carboxy-termini with respect to the side chain of theamino acid. Thus, a retro-inverso analogue has reversed termini andreversed direction of peptide bonds while approximately maintaining thetopology of the side chains as in the native peptide sequence. Theretro-inverso peptide can contain L-amino acids or D-amino acids, or amixture of L-amino acids and D-amino acids, up to all of the amino acidsbeing the D-isomer. Partial retro-inverso peptide analogues arepolypeptides in which only part of the sequence is reversed and replacedwith enantiomeric amino acid residues. Since the retro-inverted portionof such an analogue has reversed amino and carboxyl termini, the aminoacid residues flanking the retro-inverted portion are replaced byside-chain-analogous α-substituted geminal-diaminomethanes andmalonates, respectively. Retro-inverso forms of cell penetratingpeptides have been found to work as efficiently in translocating acrossa membrane as the natural forms. Synthesis of retro-inverso peptideanalogues are described in Bonelli, F. et al., Int J Pept Protein Res.24(6):553-6 (1984); Verdini, A and Viscomi, G. C., J. Chem. Soc. PerkinTrans. 1:697-701 (1985); and U.S. Pat. No. 6,261,569, which areincorporated herein in their entirety by reference. Processes for thesolid-phase synthesis of partial retro-inverso peptide analogues havebeen described (EP 97994-B) which is also incorporated herein in itsentirety by reference.

The term “fragment” of a peptide, polypeptide or molecule as used hereinrefers to any contiguous polypeptide subset of the molecule. The term“protein fragment” as used herein includes both synthetic andnaturally-occurring amino acid sequences derivable from the naturallyoccurring amino acid sequence of MIS (SEQ ID NO:1). The protein is saidto be “derivable from the naturally-occurring amino acid sequence of arecombinant human MIS protein” if it can be obtained by fragmenting therecombinant human MIS protein, or if it can be synthesized based upon aknowledge of the sequence of the naturally occurring amino acid sequenceor of the genetic material (DNA or RNA) which encodes this sequence.Accordingly, a “fragment” of a molecule, is meant to refer to anypolypeptide subset of the molecule. In some embodiments, a functionalfragment of recombinant human MIS comprises at least the C-terminaldomain and at least the N-terminal domain. In some embodiments, afunctional fragment comprises a portion of the C-terminal and/or aportion (e.g., fragment) of the N-terminal domain of the recombinanthuman MIS protein. Fragments of a recombinant human MIS protein whichhave the activity at least or greater than the wildtype MIS protein ofSEQ ID NO: 1 as disclosed herein and which are soluble are alsoencompassed for use in the present invention.

Fragments of a recombinant human MIS protein, for example functionalfragments of SEQ ID NO: 2 or 3 useful in the methods as disclosed hereinhave at least 30% the activity as that of a polypeptide of SEQ ID NO: 2or 3 in vivo, e.g., to cause Mullerian duct regression in an Mullerianduct regression bioassay as disclosed herein in the Examples. Statedanother way, a functional fragment of a recombinant human MIS protein isa fragment of any of SEQ ID NO: 2 or 3 which, alone or as a fusionprotein can result in at least 30% of the same activity as compared toSEQ ID NO: 2 or 3 to bind and activate MISRII, or cause Mullerian ductregression in a Mullerian duct regression bioassay as disclosed herein(see FIG. 4). Fragments as used herein can be soluble (i.e. not membranebound). A “fragment” can be at least about 6, at least about 9, at leastabout 15, at least about 20, at least about 30, least about 40, at leastabout 50, at least about 100, at least about 250, at least about 300nucleic or amino acids, and all integers in between. Exemplary fragmentsinclude C-terminal truncations, N-terminal truncations, or truncationsof both C- and N-terminals (e.g., deletions of, for example, at least 1,at least 2, at least 3, at least 4, at least 5, at least 8, at least 10,at least 15, at least 20, at least 25, at least 40, at least 50, atleast 75, at least 100 or more amino acids deleted from the N-termini,the C-termini, or both). One of ordinary skill in the art can createsuch fragments by simple deletion analysis. Such a fragment of SEQ IDNO:2 or 3 can be, for example, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 aminoacids or more than 10 amino acids, such as 15, 30, 50, 100 or more than100 amino acids deleted from the N-terminal and/or C-terminal of SEQ IDNO: 2 or 3, respectively. Persons of ordinary skill in the art caneasily identify the minimal peptide fragment of SEQ ID NO: 2 or 3 usefulin the methods and compositions as disclosed herein, or fusion proteinsas disclosed herein, by sequentially deleting N- and/or C-terminal aminoacids from SEQ ID NO: 2 or 3, or sequentially deleting N- and C-terminalamino acids from recombinant human MIS protein and assessing thefunction of the resulting peptide fragment, alone or when it is cleaved.One can create functional fragments with multiple smaller fragments.These can be attached by bridging peptide linkers. One can readilyselect linkers to maintain wild type conformation. One of ordinary skillin the art can easily assess the function of recombinant human MISprotein as disclosed herein to activate MISRII or in the Mullerian ductregression bioassay, as disclosed herein as compared to a recombinanthuman MIS protein corresponding to SEQ ID NO: 2 or 3. Using such an invivo assay, if the fragment of the recombinant human MIS protein has atleast 30% of the biological activity of the recombinant human MISprotein corresponding to SEQ ID NO:2 or 3 as disclosed herein, then thefragment is considered a valid recombinant human MIS protein-fragmentand can used in the compositions and methods as disclosed herein. Insome embodiments, a fragment of SEQ ID NO: 2 or 3 can be less than 200,or less than 150 or less than 100, or less than 50, or less than 20amino acids of SEQ ID NO: 2 or 3. In some embodiments, a fragment of SEQID NO: 2 or 3 is less than 100 peptides in length. However, as statedabove, the fragment must be at least 6 amino acids, at least about 9, atleast about 15, at least about 20, at least about 30, at least about 40,at least about 50, at least about 100, at least about 250, at leastabout 500 nucleic acids or amino acids, or any integers in between.

The term “derivative” as used herein refers to peptides which have beenchemically modified, for example but not limited to by techniques suchas ubiquitination, labeling, pegylation (derivatization withpolyethylene glycol) or addition of other molecules. A molecule also a“derivative” of another molecule when it contains additional chemicalmoieties not normally a part of the molecule. Such moieties can improvethe molecule's solubility, absorption, biological half life, etc. Themoieties can alternatively decrease the toxicity of the molecule,eliminate or attenuate any undesirable side effect of the molecule, etc.Moieties capable of mediating such effects are disclosed in Remington'sPharmaceutical Sciences, 18th edition, A. R. Gennaro, Ed., MackPubl.,Easton, Pa. (1990).

The term “functional” when used in conjunction with “derivative” or“variant” or “fragment” refers to a polypeptide which possess abiological activity (either functional or structural) that issubstantially similar to a biological activity of the polypeptide whichit is a functional derivative, variant or functional fragment thereof.The term functional derivative is intended to include the fragments,analogues or chemical derivatives of a molecule. By “substantiallysimilar” in this context is meant that the biological activity, e.g.,activation of MISRII is at 25% or at least 35%, or at least 50% asactive as a reference polypeptide, e.g., a corresponding wild-type MISpolypeptide or recombinant human MIS protein, and preferably at least60% as active, 70% as active, 80% as active, 90% as active, 95% asactive, 100% as active or even higher (i.e., the variant or derivativehas greater activity than the wild-type), e.g., 110% as active, 120% asactive, or more. Stated another way, a “substantially similar”functional fragment of a recombinant human MIS protein in this contextis meant that at least 25%, at least 35%, at least 50% of the relevantor desired biological activity of a corresponding recombinant human MISprotein is retained. In the instance of a functional fragment or peptideof a recombinant human MIS protein as disclosed herein (e.g., SEQ ID NO:2 or 3), a functional fragment of SEQ ID NO: 2 or 3 would be a proteinor peptide comprising a portion of SEQ ID NO: 2 or 3 which retained anactivity to activate MISRII, or in the Mullerian duct regressionbioassay, as disclosed herein in the Examples; preferably the fragmentof SEQ ID NO: 2 or 3 that retains at least 25%, at least 35%, at least50% at least 60%, at least 70%, at least 80%, at least 90%, at least95%, at least 100% or even higher (i.e., the variant or derivative hasgreater activity than the wild-type MIS of SEQ ID NO: 1 or of arecombinant human MIS protein of SEQ ID NO 2 or 3), e.g., at least 110%,at least 120%, or more activity compared to the full length SEQ ID NO: 2or 3 to activate MISRII or cause Mullerian duct regression in theMullerian duct regression bioassay as disclosed herein. As anotherexample, in the instance of a fragment of MIS (e.g., amino acids 26-560of SEQ ID NO: 1) would be a protein or peptide comprising a portion ofamino acids 26-560 of SEQ ID NO: 1 which retained an activity forMullerian duct regression, preferably the fragment of amino acids 26-560of SEQ ID NO: 1 retains at least 25%, at least 35%, at least 50% atleast 60%, at least 70%, at least 80%, at least 90%, at least 95%, atleast 100% or even higher (i.e., the variant or derivative has greateractivity than the wild-type), e.g., at least 110%, at least 120%, ormore activity compared to the full length amino acids 26-560 of SEQ IDNO: 1 to cause Mullerian duct regression in an mullerian duct regressionbioassay as disclosed herein in the Examples. As an alternative example,a fragment of a HSA leader sequence of SEQ ID NO: 6 would be a proteinor peptide comprising a portion of SEQ ID NO: 6 which retained at least25%, at least 35%, at least 50% at least 60%, at least 70%, at least80%, at least 90%, at least 95%, at least 100% or even higher (i.e., thevariant or derivative has greater activity than the wild-type HSAsequence), e.g., at least 110%, at least 120%, or more activity comparedto the full length HSA sequence of SEQ ID NO: 6, as determined by anassay, for example as disclosed in U.S. Pat. No. 5,759,802 which isincorporated herein in its entirety by reference.

The term “functional derivative” and “mimetic” or “biologically activevariant” or “biologically active fragment” are used interchangeably, andrefers to a compound which possess a biological activity (eitherfunctional or structural) that is substantially similar to a biologicalactivity of the entity or molecule its is a functional derivative of(e.g., the recombinant human MIS protein). The term functionalderivative is intended to include the fragments, variants, analogues orchemical derivatives of a molecule.

The term “functional derivatives” is intended to include the“fragments,” “variants,” “analogs,” or “chemical derivatives” of amolecule. A molecule is said to be “substantially similar” to anothermolecule if both molecules have substantially similar structures or ifboth molecules possess a similar biological activity. Thus, providedthat two molecules possess a similar activity, they are consideredvariants as that term is used herein even if the structure of one of themolecules not found in the other, or if the sequence of amino acidresidues is not identical. An “analog” of a recombinant human MISprotein is meant to refer to a molecule substantially similar infunction to either the entire molecule or to a fragment thereof. As usedherein, a molecule is said to be a “chemical derivative” of anothermolecule when it contains additional chemical moieties not normally apart of the molecule. Such moieties can improve the molecule'ssolubility, absorption, biological half life, etc. The moieties canalternatively decrease the toxicity of the molecule, eliminate orattenuate any undesirable side effect of the molecule, etc. Moietiescapable of mediating such effects are disclosed in Remington'sPharmaceutical Sciences, 18th edition, A. R. Gennaro, Ed., MackPubl.,Easton, Pa. (1990).

A “variant” of a recombinant human MIS protein is meant to refer to amolecule substantially similar in structure and function to either theentire molecule, or to a fragment thereof. Accordingly, the term“variant” as used herein refers to a peptide or nucleic acid thatdiffers from the naturally occurring polypeptide or nucleic acid by oneor more amino acid or nucleic acid deletions, additions, substitutionsor side-chain modifications, yet retains one or more specific functionsor biological activities of the naturally occurring molecule. Amino acidsubstitutions include alterations in which an amino acid is replacedwith a different naturally-occurring or a non-conventional amino acidresidue. Such substitutions may be classified as “conservative”, inwhich case an amino acid residue contained in a polypeptide is replacedwith another naturally occurring amino acid of similar character eitherin relation to polarity, side chain functionality or size. Substitutionsencompassed by the present invention may also be “non conservative”, inwhich an amino acid residue which is present in a peptide is substitutedwith an amino acid having different properties, such asnaturally-occurring amino acid from a different group (e.g.,substituting a charged or hydrophobic amino; acid with alanine), oralternatively, in which a naturally-occurring amino acid is substitutedwith a non-conventional amino acid. In some embodiments amino acidsubstitutions are conservative. Also encompassed within the term variantwhen used with reference to a polynucleotide or polypeptide, refers to apolynucleotide or polypeptide that can vary in primary, secondary, ortertiary structure, as compared to a reference polynucleotide orpolypeptide, respectively (e.g., as compared to a wild-typepolynucleotide or polypeptide). A “variant” of a recombinant human MISprotein is meant to refer to a molecule substantially similar instructure and function, i.e. where the function is the ability toactivate MISRII.

For example, a variant of a recombinant human MIS protein can contain amutation or modification that differs from a reference amino acid in SEQID NO: 2 or 3. In some embodiments, a variant of SEQ ID NO: 2 or 3 is afragment of SEQ ID NO: 2 or 3 as disclosed herein. In some embodiments,a variant can be a different isoform of SEQ ID NO: 2 or 3 or cancomprise different isomer amino acids. Variants can benaturally-occurring, synthetic, recombinant, or chemically modifiedpolynucleotides or polypeptides isolated or generated using methods wellknown in the art. Variants can include conservative or non-conservativeamino acid changes, as described below. Polynucleotide changes canresult in amino acid substitutions, additions, deletions, fusions andtruncations in the polypeptide encoded by the reference sequence.Variants can also include insertions, deletions or substitutions ofamino acids, including insertions and substitutions of amino acids andother molecules) that do not normally occur in the peptide sequence thatis the basis of the variant, for example but not limited to insertion ofornithine which do not normally occur in human proteins.

The term “conservative substitution,” when describing a polypeptide,refers to a change in the amino acid composition of the polypeptide thatdoes not substantially alter the polypeptide's activity. For example, aconservative substitution refers to substituting an amino acid residuefor a different amino acid residue that has similar chemical properties.Conservative amino acid substitutions include replacement of a leucinewith an isoleucine or valine, an aspartate with a glutamate, or athreonine with a serine. “Conservative amino acid substitutions” resultfrom replacing one amino acid with another having similar structuraland/or chemical properties, such as the replacement of a leucine with anisoleucine or valine, an aspartate with a glutamate, or a threonine witha serine. Thus, a “conservative substitution” of a particular amino acidsequence refers to substitution of those amino acids that are notcritical for polypeptide activity or substitution of amino acids withother amino acids having similar properties (e.g., acidic, basic,positively or negatively charged, polar or non-polar, etc.) such thatthe substitution of even critical amino acids does not reduce theactivity of the peptide, (i.e. the ability of the peptide to reduceT-reg cells and/or decrease inflammatory cytokines as disclosed herein).Conservative substitution tables providing functionally similar aminoacids are well known in the art. For example, the following six groupseach contain amino acids that are conservative substitutions for oneanother: 1) Alanine (A), Serine (S), Threonine (T); 2) Aspartic acid(D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine(R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine(V); and 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W). (See alsoCreighton, Proteins, W. H. Freeman and Company (1984).) In someembodiments, individual substitutions, deletions or additions thatalter, add or delete a single amino acid or a small percentage of aminoacids can also be considered “conservative substitutions” is the changedoes not reduce the activity of the MIS protein (i.e. the ability of arecombinant human MIS protein or variant to cause Mullerian ductregression in vivo, which can be determined using the Mullerian Ductregression bioassay as disclosed herein). Insertions or deletions aretypically in the range of about 1 to 5 amino acids. The choice ofconservative amino acids may be selected based on the location of theamino acid to be substituted in the peptide, for example if the aminoacid is on the exterior of the peptide and expose to solvents, or on theinterior and not exposed to solvents.

In alternative embodiments, one can select the amino acid which willsubstitute an existing amino acid based on the location of the existingamino acid, i.e. its exposure to solvents (i.e. if the amino acid isexposed to solvents or is present on the outer surface of the peptide orpolypeptide as compared to internally localized amino acids not exposedto solvents). Selection of such conservative amino acid substitutionsare well known in the art, for example as disclosed in Dordo et al, J.Mol Biol, 1999, 217, 721-739 and Taylor et al, J. Theor. Biol.119(1986); 205-218 and S. French and B. Robson, J. Mol. Evol.19(1983)171. Accordingly, one can select conservative amino acidsubstitutions suitable for amino acids on the exterior of a protein orpeptide (i.e. amino acids exposed to a solvent), for example, but notlimited to, the following substitutions can be used: substitution of Ywith F, T with S or K, P with A, E with D or Q, N with D or G, R with K,G with N or A, T with S or K, D with N or E, I with L or V, F with Y, Swith T or A, R with K, G with N or A, K with R, A with S, K or P.

In alternative embodiments, one can also select conservative amino acidsubstitutions encompassed suitable for amino acids on the interior of aprotein or peptide, for example one can use suitable conservativesubstitutions for amino acids is on the interior of a protein or peptide(i.e. the amino acids are not exposed to a solvent), for example but notlimited to, one can use the following conservative substitutions: whereY is substituted with F, T with A or S, I with L or V, W with Y, M withL, N with D, G with A, T with A or S, D with N, I with L or V, F with Yor L, S with A or T and A with S, G, T or V. In some embodiments,non-conservative amino acid substitutions are also encompassed withinthe term of variants. A variant of a recombinant human MIS protein, forexample a variant of SEQ ID NO: 2 or 3 is meant to refer to any moleculesubstantially similar in structure and function to either the entiremolecule of SEQ ID NO:2 or 3, or to a fragment thereof.

The terms “homology”, “identity” and “similarity” refer to the degree ofsequence similarity between two peptides or between two optimallyaligned nucleic acid molecules. Homology and identity can each bedetermined by comparing a position in each sequence which can be alignedfor purposes of comparison. For example, it is based upon using astandard homology software in the default position, such as BLAST,version 2.2.14. When an equivalent position in the compared sequences isoccupied by the same base or amino acid, then the molecules areidentical at that position; when the equivalent site occupied by similaramino acid residues (e.g., similar in steric and/or electronic naturesuch as, for example conservative amino acid substitutions), then themolecules can be referred to as homologous (similar) at that position.Expression as a percentage of homology/similarity or identity refers toa function of the number of similar or identical amino acids atpositions shared by the compared sequences, respectfully. A sequencewhich is “unrelated” or “non-homologous” shares less than 40% identity,though preferably less than 25% identity with the sequences as disclosedherein.

As used herein, the term “sequence identity” means that twopolynucleotide or amino acid sequences are identical (i.e., on anucleotide-by-nucleotide or residue-by-residue basis) over thecomparison window. The term “percentage of sequence identity” iscalculated by comparing two optimally aligned sequences over the windowof comparison, determining the number of positions at which theidentical nucleic acid base (e.g., A, T. C, G. U. or I) or residueoccurs in both sequences to yield the number of matched positions,dividing the number of matched positions by the total number ofpositions in the comparison window (i.e., the window size), andmultiplying the result by 100 to yield the percentage of sequenceidentity.

The terms “substantial identity” as used herein denotes a characteristicof a polynucleotide or amino acid sequence, wherein the polynucleotideor amino acid comprises a sequence that has at least 85% sequenceidentity, preferably at least 90% to 95% sequence identity, more usuallyat least 99% sequence identity as compared to a reference sequence overa comparison window of at least 18 nucleotide (6 amino acid) positions,frequently over a window of at least 24-48 nucleotide (8-16 amino acid)positions, wherein the percentage of sequence identity is calculated bycomparing the reference sequence to the sequence which can includedeletions or additions which total 20 percent or less of the referencesequence over the comparison window. The reference sequence can be asubset of a larger sequence. The term “similarity”, when used todescribe a polypeptide, is determined by comparing the amino acidsequence and the conserved amino acid substitutes of one polypeptide tothe sequence of a second polypeptide.

As used herein, the terms “homologous” or “homologues” are usedinterchangeably, and when used to describe a polynucleotide orpolypeptide, indicates that two polynucleotides or polypeptides, ordesignated sequences thereof, when optimally aligned and compared, forexample using BLAST, version 2.2.14 with default parameters for analignment (see herein) are identical, with appropriate nucleotideinsertions or deletions or amino-acid insertions or deletions, in atleast 70% of the nucleotides, usually from about 75% to 99%, and morepreferably at least about 98 to 99% of the nucleotides. The term“homolog” or “homologous” as used herein also refers to homology withrespect to structure and/or function. With respect to sequence homology,sequences are homologs if they are at least 50%, at least 60 at least70%, at least 80%, at least 90%, at least 95% identical, at least 97%identical, or at least 99% identical. Determination of homologs of thegenes or peptides of the present invention can be easily ascertained bythe skilled artisan.

The term “substantially homologous” refers to sequences that are atleast 90%, at least 95% identical, at least 96%, identical at least 97%identical, at least 98% identical or at least 99% identical. Homologoussequences can be the same functional gene in different species.Determination of homologs of the genes or peptides of the presentinvention can be easily ascertained by the skilled artisan.

A molecule is said to be “substantially similar” to another molecule ifboth molecules have substantially similar structures or if bothmolecules possess a similar biological activity, for example if bothmolecules are able to activate MISRII. Thus, provided that two moleculespossess a similar activity, (i.e. a variant of a recombinant human MISprotein which can activate MISRII similar to that of the MIS proteinwhich corresponds to SEQ ID NO: 1, or recombinant human MIS proteinwhich corresponds to SEQ ID NO: 2 or 3) are considered variants and areencompassed for use as disclosed herein, even if the structure of one ofthe molecules not found in the other, or if the sequence of amino acidresidues is not identical. Thus, provided that two molecules possess asimilar biological activity, they are considered variants as that termis used herein even if the structure of one of the molecules not foundin the other, or if the sequence of amino acid residues is notidentical. In particular, the term “substantially similar”, when used todefine a recombinant human MIS protein comprising a functional variantof recombinant human MIS protein as compared to the recombinant humanMIS protein encoded by SEQ ID NO:2 or 3, means that a particular subjectsequence, for example, a recombinant human MIS protein variant orderivative sequence, varies from the sequence of the natural (orwild-type) MIS of SEQ ID NO: 1 or recombinant human MIS protein (i.e.encoded by SEQ ID NO: 2 or 3), by one or more substitutions, deletions,or additions, although the net effect of which is to retain at leastsome of the biological activity found in the recombinant human MISprotein as disclosed herein. As such, nucleic acid and amino acidsequences having lesser degrees of similarity but comparable biologicalactivity to recombinant human MIS protein are considered to beequivalents. In determining polynucleotide sequences, all subjectpolynucleotide sequences capable of encoding substantially similar aminoacid sequences are considered to be substantially similar to a referencepolynucleotide sequence, regardless of differences in codon sequence. Anucleotide sequence is “substantially similar” to a specific nucleicacid sequence of SEQ ID NO:4 or 5 as disclosed herein if: (a) thenucleotide sequence is hybridizes to the coding regions of the naturalMIS nucleic acid, or (b) the nucleotide sequence is capable ofhybridization to nucleotide sequence of a recombinant human MIS proteinencoded by SEQ ID NO: 4 or 5 under moderately stringent conditions andhas biological activity similar to the recombinant human MIS protein; or(c) the nucleotide sequences which are degenerative as a result of thegenetic code to the nucleotide sequences defined in (a) or (b).Substantially similar proteins will typically be greater than about 80%similar to the corresponding sequence of the native protein.

The term “substantial similarity” in the context of polypeptidesequences, indicates that the polypeptide comprises a sequence with atleast 60% sequence identity to a reference sequence, or 70%, or 80%, or85% sequence identity to the reference sequence, or most preferably 90%identity over a comparison window of about 10-20 amino acid residues. Inthe context of amino acid sequences, “substantial similarity” furtherincludes conservative substitutions of amino acids. Thus, a polypeptideis substantially similar to a second polypeptide, for example, where thetwo peptides differ by one or more conservative substitutions.

In one embodiment, the term “human homolog” to a gene transcript refersto a DNA sequence that has at least about 55% homology to the fulllength nucleotide sequence of the sequence of a recombinant human MISprotein gene as encoded by the genome of humans or an animal, forexample mouse or transgenic animal. In one embodiment, the term “humanhomolog” to a protein identified as associated with a recombinant humanMIS protein refers to an amino acid sequence that has 40% homology tothe full length amino acid sequence of the protein identified asassociated with a recombinant human MIS protein as encoded by the genomeof the transgenic animal of the present invention, more preferably atleast about 50%, still more preferably, at least about 60% homology,still more preferably, at least about 70% homology, even morepreferably, at least about 75% homology, yet more preferably, at leastabout 80% homology, even more preferably at least about 85% homology,still more preferably, at least about 90% homology, and more preferably,at least about 95% homology. As discussed above, the homology is atleast about 50% to 100% and all intervals in between (i.e., 55%, 60%,70%, 75%, 80%, 85%, 90%, 95%, 98%, etc.). Determination of the humanhomologs of the genes of the present invention may be easily ascertainedby the skilled artisan.

The term “conservative substitution,” when describing a polypeptide,refers to a change in the amino acid composition of the polypeptide thatdoes not substantially alter the polypeptide's activity. Thus, a“conservative substitution” of a particular amino acid sequence refersto substitution of those amino acids that are not critical forpolypeptide activity or substitution of amino acids with other aminoacids having similar properties (e.g., acidic, basic, positively ornegatively charged, polar or non-polar, etc.) such that the substitutionof even critical amino acids does not substantially alter activity.Conservative substitution tables providing functionally similar aminoacids are well known in the art. For example, the following six groupseach contain amino acids that are conservative substitutions for oneanother: 1) Alanine (A), Serine (S), Threonine (T); 2) Aspartic acid(D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine(R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine(V); and 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W). (See alsoCreighton, Proteins, W. H. Freeman and Company (1984).) In addition,individual substitutions, deletions or additions that alter, add ordelete a single amino acid or a small percentage of amino acids in anencoded sequence are also “conservative substitutions.”

As used herein, the term “nonconservative” refers to substituting anamino acid residue for a different amino acid residue that has differentchemical properties. The nonconservative substitutions include, but arenot limited to aspartic acid (D) being replaced with glycine (G);asparagine (N) being replaced with lysine (K); or alanine (A) beingreplaced with arginine (R).

For sequence comparison, typically one sequence acts as a referencesequence, to which test sequences are compared. When using a sequencecomparison algorithm, test and reference sequences are input into acomputer, subsequence coordinates are designated, if necessary, andsequence algorithm program parameters are designated. The sequencecomparison algorithm then calculates the percent sequence identity forthe test sequence(s) relative to the reference sequence, based on thedesignated program parameters.

Optimal alignment of sequences for comparison can be conducted, forexample, by the local homology algorithm of Smith and Waterman (Adv.Appl. Math. 2:482 (1981), which is incorporated by reference herein), bythe homology alignment algorithm of Needleman and Wunsch (J. Mol. Biol.48:443-53 (1970), which is incorporated by reference herein), by thesearch for similarity method of Pearson and Lipman (Proc. Natl. Acad.Sci. USA 85:2444-48 (1988), which is incorporated by reference herein),by computerized implementations of these algorithms (e.g., GAP, BESTFIT,FASTA, and TFASTA in the Wisconsin Genetics Software Package, GeneticsComputer Group, 575 Science Dr., Madison, Wis.), or by visualinspection. (See generally Ausubel et al. (eds.), Current Protocols inMolecular Biology, 4th ed., John Wiley and Sons, New York (1999)).

One example of a useful algorithm is PILEUP. PILEUP creates a multiplesequence alignment from a group of related sequences using progressive,pairwise alignments to show the percent sequence identity. It also plotsa tree or dendogram showing the clustering relationships used to createthe alignment. PILEUP uses a simplification of the progressive alignmentmethod of Feng and Doolittle (J. Mol. Evol. 25:351-60 (1987), which isincorporated by reference herein). The method used is similar to themethod described by Higgins and Sharp (Comput. Appl. Biosci. 5:151-53(1989), which is incorporated by reference herein). The program canalign up to 300 sequences, each of a maximum length of 5,000 nucleotidesor amino acids. The multiple alignment procedure begins with thepairwise alignment of the two most similar sequences, producing acluster of two aligned sequences. This cluster is then aligned to thenext most related sequence or cluster of aligned sequences. Two clustersof sequences are aligned by a simple extension of the pairwise alignmentof two individual sequences. The final alignment is achieved by a seriesof progressive, pairwise alignments. The program is run by designatingspecific sequences and their amino acid or nucleotide coordinates forregions of sequence comparison and by designating the programparameters. For example, a reference sequence can be compared to othertest sequences to determine the percent sequence identity relationshipusing the following parameters: default gap weight (3.00), default gaplength weight (0.10), and weighted end gaps.

Another example of an algorithm that is suitable for determining percentsequence identity and sequence similarity is the BLAST algorithm, whichis described by Altschul et al. (J. Mol. Biol. 215:403-410 (1990), whichis incorporated by reference herein). (See also Zhang et al., NucleicAcid Res. 26:3986-90 (1998); Altschul et al., Nucleic Acid Res.25:3389-402 (1997), which are incorporated by reference herein).Software for performing BLAST analyses is publicly available through theNational Center for Biotechnology Information internet web site. Thisalgorithm involves first identifying high scoring sequence pairs (HSPs)by identifying short words of length W in the query sequence, whicheither match or satisfy some positive-valued threshold score T whenaligned with a word of the same length in a database sequence. T isreferred to as the neighborhood word score threshold (Altschul et al.(1990), supra). These initial neighborhood word hits act as seeds forinitiating searches to find longer HSPs containing them. The word hitsare then extended in both directions along each sequence for as far asthe cumulative alignment score can be increased. Extension of the wordhits in each direction is halted when: the cumulative alignment scorefalls off by the quantity X from its maximum achieved value; thecumulative score goes to zero or below, due to the accumulation of oneor more negative-scoring residue alignments; or the end of eithersequence is reached. The BLAST algorithm parameters W, T, and Xdetermine the sensitivity and speed of the alignment. The BLAST programuses as defaults a wordlength (W) of 11, the BLOSUM62 scoring matrix(see Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915-9(1992), which is incorporated by reference herein) alignments (B) of 50,expectation (E) of 10, M=5, N=−4, and a comparison of both strands.

In addition to calculating percent sequence identity, the BLASTalgorithm also performs a statistical analysis of the similarity betweentwo sequences (see, e.g., Karlin and Altschul, Proc. Natl. Acad. Sci.USA 90:5873-77 (1993), which is incorporated by reference herein). Onemeasure of similarity provided by the BLAST algorithm is the smallestsum probability (P(N)), which provides an indication of the probabilityby which a match between two nucleotide or amino acid sequences wouldoccur by chance. For example, a nucleic acid is considered similar to areference sequence if the smallest sum probability in a comparison ofthe test nucleic acid to the reference nucleic acid is less than about0.1, more typically less than about 0.01, and most typically less thanabout 0.001.

The term “insertions” or “deletions” are typically in the range of about1 to 5 amino acids. The variation allowed can be experimentallydetermined by producing the peptide synthetically while systematicallymaking insertions, deletions, or substitutions of nucleotides in thesequence using recombinant DNA techniques.

The term “substitution” when referring to a peptide, refers to a changein an amino acid for a different entity, for example another amino acidor amino-acid moiety. Substitutions can be conservative ornon-conservative substitutions.

An “analog” of a molecule such as a recombinant human MIS protein, forexample SEQ ID NO: 2 or 3 refers to a molecule similar in function toeither the entire molecule or to a fragment thereof. The term “analog”is also intended to include allelic, species and induced variants.Analogs typically differ from naturally occurring peptides at one or afew positions, often by virtue of conservative substitutions. Analogstypically exhibit at least 80 or 90% sequence identity with naturalpeptides. Some analogs also include unnatural amino acids ormodifications of N or C terminal amino acids. Examples of unnaturalamino acids are, for example but not limited to; acedisubstituted aminoacids, N-alkyl amino acids, lactic acid, 4-hydroxyproline,γ-carboxyglutamate, ε-N,N,N-trimethyllysine, ε-N-acetyllysine,O-phosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine,5-hydroxylysine, σ-N-methylarginine. Fragments and analogs can bescreened for prophylactic or therapeutic efficacy in transgenic animalmodels as described below.

By “covalently bonded” is meant joined either directly or indirectly(e.g., through a linker) by a covalent chemical bond.

The term “fusion protein” as used herein refers to a recombinant proteinof two or more proteins. Fusion proteins can be produced, for example,by a nucleic acid sequence encoding one protein is joined to the nucleicacid encoding another protein such that they constitute a singleopen-reading frame that can be translated in the cells into a singlepolypeptide harboring all the intended proteins. The order ofarrangement of the proteins can vary. As a non-limiting example, thenucleic acid sequence encoding the recombinant human MIS-fusion proteinis derived from the nucleotide sequence of encoding a recombinant humanMIS protein or a functional derivative fragment or variant thereof,fused in frame to an end, either the 5′ or the 3′ end, of a geneencoding a first fusion partner, such as a IgG1 Fc fragment. In thismanner, on expression of the gene, the recombinant human MIS protein orfunctional derivative fragment or variant thereof is functionallyexpressed and fused to the N-terminal or C-terminal end of the IgG1 Fc.In certain embodiments, modification of the polypeptide probe is suchthat the functionality of the recombinant human MIS protein or afunctional derivative fragment or variant thereof remains substantiallyunaffected in terms of its biological activity by fusion to the firstfusion partner, such as IgG1 Fc.

By “specifically binds” or “specific binding” is meant a compound orantibody that recognizes and binds a desired polypeptide but that doesnot substantially recognize and bind other molecules in a sample, forexample, a biological sample, which naturally includes a polypeptide ofthe invention.

By “substantially pure” or is meant a nucleic acid, polypeptide, orother molecule that has been separated from the components thatnaturally accompany it. Typically, a polypeptide is substantially purewhen it is at least about 60%, or at least about 70%, at least about80%, at least about 90%, at least about 95%, or even at least about 99%,by weight, free from the proteins and naturally-occurring organicmolecules with which it is naturally associated. For example, asubstantially pure polypeptide may be obtained by extraction from anatural source, by expression of a recombinant nucleic acid in a cellthat does not normally express that protein, or by chemical synthesis.

By “enhanced proteolytic stability” is meant a reduction of in the rateor extent of proteolysis of a peptide sequence by at least about 2%, atleast about 5%, at least about 10%, at least about 20%, at least about30%, at least about 40%, at least about 50%, at least about 60%, atleast about 70%, at least about 80%, at least about 85%, at least about90%, at least about 95%, at least about 98%, or at least about 99% ascompared to a control sequence under the same conditions (e.g., in vivoor in an in vitro system such as in a cell or cell lysate). A peptidewith enhanced proteolytic stability may contain any modification, forexample, insertions, deletions, or point mutations which reduce oreliminate a site subject to proteolytic cleavage at a particular site.Sites of proteolytic cleavage may be identified based on known targetsequences or using computer software (e.g., software described byGasteiger et al., Protein Identification and Analysis Tools on theExPASy Server. In John M. Walker, ed. The Proteomics Protocols Handbook,Humana Press (2005)). Alternatively, proteolytic sites can be determinedexperimentally, for example, by Western blot for the protein followingexpression or incubation in a cellular system or cellular lysate,followed by sequencing of the identified fragments to determine cleavagesites.

The term “recombinant” as used herein to describe a nucleic acidmolecule, means a polynucleotide of genomic, cDNA, viral, semisynthetic,and/or synthetic origin, which, by virtue of its origin or manipulation,is not associated with all or a portion of the polynucleotide with whichit is associated in nature. The term recombinant as used with respect toa protein or polypeptide, means a polypeptide produced by expression ofa recombinant polynucleotide. The term recombinant as used with respectto a host cell means a host cell into which a recombinant polynucleotidehas been introduced. Recombinant is also used herein to refer to, withreference to material (e.g., a cell, a nucleic acid, a protein, or avector) that the material has been modified by the introduction of aheterologous material (e.g., a cell, a nucleic acid, a protein, or avector).

The terms “subject” and “individual” are used interchangeably herein,and refer to an animal, for example a human, to whom treatment,including prophylactic treatment, with the pharmaceutical compositionaccording to the present invention, is provided. The term “subject” asused herein refers to human and non-human animals. The term “non-humananimals” and “non-human mammals” are used interchangeably hereinincludes all vertebrates, e.g., mammals, such as non-human primates,(particularly higher primates), sheep, dog, rodent (e.g. mouse or rat),guinea pig, goat, pig, cat, rabbits, cows, and non-mammals such aschickens, amphibians, reptiles etc. In one embodiment, the subject ishuman. In another embodiment, the subject is an experimental animal oranimal substitute as a disease model. The term does not denote aparticular age or sex. Thus, adult and newborn subjects, as well asfetuses, whether male or female, are intended to be covered. Examples ofsubjects include humans, dogs, cats, cows, goats, and mice. The termsubject is further intended to include transgenic species. The termsubject also encompasses a mammal, for example, a human, to whomtreatment, such as therapeutic treatment and/or prophylactic treatmentwith a composition comprising a recombinant human MIS protein asdisclosed herein is provided.

The term “tissue” is intended to include intact cells, blood, bloodpreparations such as plasma and serum, bones, joints, muscles, smoothmuscles, and organs.

The term “disease” or “disorder” is used interchangeably herein, refersto any alternation in state of the body or of some of the organs,interrupting or disturbing the performance of the functions and/orcausing symptoms such as discomfort, dysfunction, distress, or evendeath to the person afflicted or those in contact with a person. Adisease or disorder can also related to a distemper, ailing, ailment,amlady, disorder, sickness, illness, complaint, inderdisposion,affection.

The term “malignancy” and “cancer” are used interchangeably herein,refers to diseases that are characterized by uncontrolled, abnormalgrowth of cells. Cancer cells can spread locally or through thebloodstream and lymphatic system to other parts of the body. The term isalso intended to include any disease of an organ or tissue in mammalscharacterized by poorly controlled or uncontrolled multiplication ofnormal or abnormal cells in that tissue and its effect on the body as awhole. Cancer diseases within the scope of the definition comprisebenign neoplasms, dysplasias, hyperplasias as well as neoplasms showingmetastatic growth or any other transformations like e.g. leukoplakiaswhich often precede a breakout of cancer.

As used herein, the term “tumor” refers to a mass of transformed cellsthat are characterized, at least in part, by containing angiogenicvasculature. The transformed cells are characterized by neoplasticuncontrolled cell multiplication which is rapid and continues even afterthe stimuli that initiated the new growth has ceased. The term “tumor”is used broadly to include the tumor parenchymal cells as well as thesupporting stroma, including the angiogenic blood vessels thatinfiltrate the tumor parenchymal cell mass. Although a tumor generallyis a malignant tumor, i.e., a cancer having the ability to metastasize(i.e. a metastatic tumor), a tumor also can be nonmalignant (i.e.non-metastatic tumor). Tumors are hallmarks of cancer, a neoplasticdisease the natural course of which is fatal. Cancer cells exhibit theproperties of invasion and metastasis and are highly anaplastic.

As used herein, the term “metastases” or “metastatic tumor” refers to asecondary tumor that grows separately elsewhere in the body from theprimary tumor and has arisen from detached, transported cells, whereinthe primary tumor is a solid tumor. The primary tumor, as used herein,refers to a tumor that originated in the location or organ in which itis present and did not metastasize to that location from anotherlocation. As used herein, a “malignant tumor” is one having theproperties of invasion and metastasis and showing a high degree ofanaplasia. Anaplasia is the reversion of cells to an immature or a lessdifferentiated form, and it occurs in most malignant tumors.

The term “therapy resistant cancer” as used herein refers to a cancerpresent in a subject which is resistant to, or refractory to at leasttwo different anti-cancer agents such as chemotherapy agents, whichmeans, typically a subject has been treated with at least two differentanti-cancer agents that did not provide effective treatment as that termis defined herein.

The term ‘sensitize’ or ‘sensitizes’ used interchangeably herein, refersto making the cell sensitive, or susceptible to other secondary agents,for example other pro-drugs or other environmental effects such asradiation etc.

The term “disease” or “disorder” is used interchangeably herein, andrefers to any alteration in state of the body or of some of the organs,interrupting or disturbing the performance of the functions and/orcausing symptoms such as discomfort, dysfunction, distress, or evendeath to the person afflicted or those in contact with a person. Adisease or disorder can also relate to a distemper, ailing, ailment,malady, disorder, sickness, illness, complaint, inderdisposion oraffectation.

The term “neurodegenerative disease” as used herein refers to a variedassortment of central and peripheral nervous system disorderscharacterized by gradual and progressive loss of neural tissue and/orneural tissue function. A neurodegenerative disease is characterized byan increase in neuronal cell death or impairment, or an increase indysfunctional or degenerating neurons. A neurodegenerative disease is aclass of neurological disorders or diseases, and where the neurologicaldisease is characterized by a gradual and progressive loss of neuraltissue, and/or altered neurological function, typically reducedneurological function as a result of a gradual and progressive loss ofneural tissue. Examples of neurodegenerative diseases include forexample, but are not limited to, conditions where neurons aredysfunctional and/or degenerating, including but not limited to,neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS),primary lateral sclerosis (PLS), spinal muscular atrophy (SMA),Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease(HD), Friedreich's ataxia, cerebellar ataxia, other brain disorders suchas bipolar disorder, epilepsy, schizophrenia, depression, mania, autism,ADHD, brain trauma injuries and stroke.

In some embodiments, the present invention provides a method oftreatment, or prevention or diagnosis of conditions where neurons aredysfunctional and/or degenerating, including but not limited to,neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS),primary lateral sclerosis (PLS), spinal muscular atrophy (SMA),Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease(HD), Friedreich's ataxia, cerebellar ataxia, other brain disorders suchas bipolar disorder, epilepsy, schizophrenia, depression, mania, autism,ADHD, brain trauma injuries and stroke

The phrase “motor neuron disease” or “MND” as used herein, refers to aneurological disorder that selectively destroys motor neurons, the cellsthat control voluntary muscle activity including speaking, walking,swallowing, and general movement of the body. MNDs are generallyprogressive in nature, and cause increasingly debilitating disabilityand, eventually, death. Encompassed in the term motor neuron diseasesinclude, but are not limited to, Amyotrophic Lateral Sclerosis (ALS),also known as Lou Gehrig's Disease, primary lateral sclerosis (PLS),progressive muscular atrophy (PMA), pseudobulbar palsy, progressivebulbar palsy, lower motor neuron disease and spinal muscular atrophy 1The term “motor neuron disease” applies to disorders which affect eitherupper motor neurons (UMN) in the brain or lower motor neurons (LMN) inthe spinal cord, or both. Motor neuron diseases which affect the UMNonly, include, for example, Primary lateral sclerosis (PLS),Pseudobulbar palsy and Hereditary spastic paraplegia. Motor neuronswhich affect the LMN only include, for example, Distal hereditary motorneuropathies, Spinal muscular atrophies (SMA ⋅SMAX1 ⋅SMAX2 ⋅DSMA1⋅Congenital DSMA ⋅SMA-PCH ⋅SMA-LED ⋅SMA-PME), Progressive muscularatrophy, progressive bulbar palsy (Fazio-Londe and Infantile progressivebulbar palsy). Motor neurons which affect both the UMN and LMN include,but is not limited to Amyotrophic lateral sclerosis (ALS). The termmotor neuron disease also encompasses spinal muscular atrophies, such asspinal muscular atrophy (SMA) (SMA1, Werdnig-Hoffmann Disease), SpinalMuscular Atrophy Type 2 (SMA2) and Spinal Muscular Atrophy Type 3 (SMA3,Kugelberg-Welander Disease), spinobulbar muscular atrophy andCharcot-Marie-Tooth Disorders.

The term “motor neuron” also referred to as a “motoneuron” refers to aneuron that sends electrical output signals to a muscle, gland, or othereffector tissues.

As used herein, the term “diagnosing” refers to classifying a pathology(e.g., a disease, disorder, syndrome, medical condition and/or a symptomthereof), determining a severity of the pathology, monitoring theprogression of a pathology, forecasting an outcome of the pathologyand/or prospects of recovery (e.g., prognosis).

As used herein, the terms “treat” or “treatment” or “treating” refers toboth therapeutic treatment and prophylactic or preventative measures,wherein the object is to prevent or slow the development of the disease,such as, in cancer, slow down the development of a tumor, prevent ordecrease the spread of cancer, or reducing at least one effect orsymptom of a condition, disease or disorder associated withinappropriate proliferation or a cell mass, for example cancer, or inneurodegenerative diseases, slow or decrease the rate of degeneration,impairment or cell death of neurons, or prevent neuronal cell death fromoccurring. Treatment is generally “effective” if one or more symptoms orclinical markers are reduced as that term is defined herein.Alternatively, treatment is “effective” if the progression of a diseaseis reduced or halted. That is, “treatment” includes not just theimprovement of symptoms or markers, but also a measurable lessening ofone or more symptoms or measurable markers of a disease or disorder(e.g., cancer) and/or a cessation of at least slowing of progress orworsening of symptoms that would be expected in absence of treatment.Measurable lessening includes any statistically significant decline in ameasurable marker or symptom. Beneficial or desired clinical resultsinclude, but are not limited to, alleviation of one or more symptom(s),diminishment of extent of disease, stabilized (i.e., not worsening)state of disease, delay or slowing of disease progression, ameliorationor palliation of the disease state, and remission (whether partial ortotal), whether detectable or undetectable. “Treatment” can also meanprolonging survival as compared to expected survival if not receivingtreatment. Those in need of treatment include those already diagnosedwith cancer, as well as those likely to develop secondary tumors due tometastasis. Those in need of treatment include those already diagnosedwith a neurodegenerative disease or disorder, as well as those likely todevelop a neurodegenerative disease or disorder, e.g., a subject havinga genetic mutation which increases the predisposition to aneurodegenerative disease or disorder. For example, a subject with amutation in the SOD1 gene. Accordingly, in some embodiments, where thesubject is likely to get cancer, or has a mutation in a gene thatincreases their likelihood to develop a neurodegenerative disease ordisorder such as ALS, HD or AD, the treatment can be prophylactictreatment.

Thus, one of skill in the art realizes that a treatment with arecombinant MIS as disclosed herein may improve the disease condition,but may not be a complete cure for the disease. In some embodiments,treatment can be “prophylaxic treatment, where the subject isadministered a composition as disclosed herein (e.g., a recombinant MISprotein or viral vector encoding the same) to a subject at risk ofdeveloping a motor neuron disease as disclosed herein. In someembodiments, treatment is “effective” if the progression of a disease isreduced or halted. Those in need of treatment include those alreadydiagnosed with a neurodegenerative disease or disorder, or motor neurondisease or disorder, e.g., ALS or SMA, as well as those likely todevelop a neurodegenerative disease or motor neuron disease or disorderdue to genetic susceptibility or other factors such as family history ofmotor neuron disease, exposure to susceptibility factors, weight, dietand health.

As used herein, the term “treating” when used in reference to a cancertreatment is used to refer to the reduction of a symptom and/or abiochemical marker of cancer, for example a significant reduction in atleast one biochemical marker of cancer would be considered an effectivetreatment. Examples of such biochemical markers of cancer include CD44,telomerase, TGF-α, TGF-β, erbB-2, erbB-3, MUC1, MUC2, CK20, PSA, CA125and FOBT. A reduction in the rate of proliferation of the cancer cellsby at least about 10% would also be considered effective treatment bythe methods as disclosed herein. As alternative examples, a reduction ina symptom of cancer, for example, a slowing of the rate of growth of thecancer by at least about 10% or a cessation of the increase in tumorsize, or a reduction in the size of a tumor by at least about 10% or areduction in the tumor spread (i.e. tumor metastasis) by at least about10% would also be considered as affective treatments by the methods asdisclosed herein. In some embodiments, it is preferred, but not requiredthat the therapeutic agent actually kill the tumor.

With reference to the treatment of a subject with a cancer with apharmaceutical composition comprising at least one recombinant human MISprotein as disclosed herein, the term “therapeutically effective amount”refers to the amount that is safe and sufficient to prevent or delay thedevelopment and further growth of a tumor or the spread of metastases incancer patients. The amount can thus cure or cause the cancer to go intoremission, slow the course of cancer progression, slow or inhibit tumorgrowth, slow or inhibit tumor metastasis, slow or inhibit theestablishment of secondary tumors at metastatic sites, or inhibit theformation of new tumor metastases. The effective amount for thetreatment of cancer depends on the tumor to be treated, the severity ofthe tumor, the drug resistance level of the tumor, the species beingtreated, the age and general condition of the subject, the mode ofadministration and so forth. Thus, it is not possible to specify theexact “effective amount”. However, for any given case, an appropriate“effective amount” can be determined by one of ordinary skill in the artusing only routine experimentation. The efficacy of treatment can bejudged by an ordinarily skilled practitioner, for example, efficacy canbe assessed in animal models of cancer and tumor, for example treatmentof a rodent with a cancer, and any treatment or administration of thecompositions or formulations that leads to a decrease of at least onesymptom of the cancer, for example a reduction in the size of the tumoror a slowing or cessation of the rate of growth of the tumor indicateseffective treatment. In embodiments where the compositions are used forthe treatment of cancer, the efficacy of the composition can be judgedusing an experimental animal model of cancer, e.g., wild-type mice orrats, or preferably, transplantation of tumor cells. When using anexperimental animal model, efficacy of treatment is evidenced when areduction in a symptom of the cancer, for example a reduction in thesize of the tumor or a slowing or cessation of the rate of growth of thetumor occurs earlier in treated, versus untreated animals. By “earlier”is meant that a decrease, for example in the size of the tumor occurs atleast 5% earlier, but preferably more, e.g., one day earlier, two daysearlier, 3 days earlier, or more.

The term “effective amount” as used herein refers to the amount of arecombinant human MIS protein as disclosed herein, to alleviate at leastone or more symptom of the disease or disorder, and relates to asufficient amount of pharmacological composition to provide the desiredeffect. The phrase “therapeutically effective amount” as used herein,e.g., a pharmaceutical composition comprising at least one recombinanthuman MIS protein as disclosed herein means a sufficient amount of thecomposition to treat a disorder, at a reasonable benefit/risk ratioapplicable to any medical treatment. The term “therapeutically effectiveamount” therefore refers to an amount of the composition as disclosedherein that is sufficient to effect a therapeutically orprophylactically significant reduction in a symptom or clinical markerassociated with a cancer or a cancer-mediated condition.

A therapeutically or prophylactically significant reduction in a symptomis, e.g. at least about 10%, at least about 20%, at least about 30%, atleast about 40%, at least about 50%, at least about 60%, at least about70%, at least about 80%, at least about 90%, at least about 100%, atleast about 125%, at least about 150% or more in a measured parameter ascompared to a control or non-treated subject. Measured or measurableparameters include clinically detectable markers of disease, forexample, elevated or depressed levels of a biological marker, as well asparameters related to a clinically accepted scale of symptoms or markersfor a disease or disorder. It will be understood, however, that thetotal daily usage of the compositions and formulations as disclosedherein will be decided by the attending physician within the scope ofsound medical judgment. The exact amount required will vary depending onfactors such as the type of disease being treated.

The term “prophylactically effective amount” refers to an amount of arecombinant human MIS protein or functional fragment or variant thereofwhich is effective, at dosages and for periods of time necessary, toachieve the desired prophylactic result, e.g., to prevent the onset ofcancer in a subject who is at risk of developing cancer, or to preventthe onset of muscle weakness and/or decrease in neurological function ina subject who is at risk of developing a neurodegenerative disease ormotor neuron disease. Typically, since a prophylactic dose of arecombinant human MIS protein or functional fragment or variant thereofis administered to a subject prior to, or at an earlier stage of acancer or neurodegenerative disease, or to a subject who has a geneticpredisposition to get cancer or a neurodegenerative disease, forexample, but by no way a limitation, to a subject that has a mutation ina gene which increases the likelihood of the subject getting cancer,e.g., ovarian cancer such as a mutation in the BRCA1 and/or BRAC2 gene,or a subject who has a mutation in a gene which increases the likelihoodof the subject getting a neurodegenerative disease, such as ALS, e.g., asubject who has mutation in the SOD1 gene. In some embodiments, aprophylactically effective amount is less than the therapeuticallyeffective amount. A prophylactically effective amount of a recombinanthuman MIS protein or functional fragment or variant thereof is also onein which any toxic or detrimental effects of the compound are outweighedby the beneficial effects.

As used herein, the terms “prevent,” “preventing” and “prevention” referto the avoidance or delay in manifestation of one or more symptoms ormeasurable markers of a disease or disorder, e.g., of an autoimmunedisease. A delay in the manifestation of a symptom or marker is a delayrelative to the time at which such symptom or marker manifests in acontrol or untreated subject with a similar likelihood or susceptibilityof developing the disease or disorder. The terms “prevent,” “preventing”and “prevention” include not only the avoidance or prevention of asymptom or marker of the disease, but also a reduced severity or degreeof any one of the symptoms or markers of the disease, relative to thosesymptoms or markers in a control or non-treated individual with asimilar likelihood or susceptibility of developing the disease ordisorder, or relative to symptoms or markers likely to arise based onhistorical or statistical measures of populations affected by thedisease or disorder. By “reduced severity” is meant at least a 10%reduction in the severity or degree of a symptom or measurable diseasemarker, relative to a control or reference, e.g., at least 15%, 20%,30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99% or even 100% (i.e., nosymptoms or measurable markers).

As used herein, the terms “administering,” and “introducing” are usedinterchangeably herein and refer to the placement of the recombinant MISprotein, or an agent or vector expressing the recombinant MIS protein asdisclosed herein into a subject by a method or route which results in atleast partial localization of a recombinant human MIS protein at adesired site. The compounds of the present invention can be administeredby any appropriate route which results in an effective treatment in thesubject. In some embodiments, for the treatment of a cancer, therecombinant human MIS protein can be placed directly at, or near thesite of the tumor or alternatively administered systemically. In someembodiments, for the treatment of a neurodegenerative disease ordisorder, the recombinant human MIS protein can be placed directly inthe spinal cord or in the cerebellum, or alternatively be administeredby any appropriate route which results in delivery to a desired locationin the subject where the recombinant human MIS protein can be deliveredto the degenerating neurons, e.g., such as intravenous administration ofAAV expressing the recombinant human MIS protein enables recombinanthuman MIS protein expression in the brain and spinal cord.

A “composition” or “pharmaceutical composition” are used interchangeablyherein refers to a composition that usually contains an excipient, suchas a pharmaceutically acceptable carrier that is conventional in the artand that is suitable for administration to cells. The cells may be partof a subject, for example for therapeutic, diagnostic, or prophylacticpurposes. The cells may also be cultured, for example cells as part ofan assay for screening potential pharmaceutical compositions, and thecells may be part of a transgenic animal for research purposes. Thecomposition can also be a cell culture, in which a polypeptide orpolynucleotide encoding a metabolic regulator of the present inventionis present in the cells and/or in the culture medium. In addition,compositions for topical (e.g., oral mucosa, respiratory mucosa) and/ororal administration can form solutions, suspensions, tablets, pills,capsules, sustained-release formulations, oral rinses, or powders, asknown in the art and described herein. The compositions also can includestabilizers and preservatives. For examples of carriers, stabilizers andadjuvants, University of the Sciences in Philadelphia (2005) Remington:The Science and Practice of Pharmacy with Facts and Comparisons, 21stEd.

The phrases “parenteral administration” and “administered parenterally”as used herein means modes of administration other than enteral andtopical administration, usually by injection, and includes, withoutlimitation, intravenous, intramuscular, intraarterial, intrathecal,intraventricular, intracapsular, intraorbital, intracardiac,intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular,intraarticular, sub capsular, subarachnoid, intraspinal, intracerebrospinal, and intrasternal injection and infusion. The phrases “systemicadministration,” “administered systemically”, “peripheraladministration” and “administered peripherally” as used herein mean theadministration of a recombinant human MIS protein such that it entersthe animal's system and, thus, is subject to metabolism and other likeprocesses, for example, subcutaneous administration.

The phrase “pharmaceutically acceptable” is employed herein to refer tothose compounds, materials, compositions, and/or dosage forms which are,within the scope of sound medical judgment, suitable for use in contactwith the tissues of human beings and animals without excessive toxicity,irritation, allergic response, or other problem or complication,commensurate with a reasonable benefit/risk ratio.

The phrase “pharmaceutically acceptable carrier” as used herein means apharmaceutically acceptable material, composition or vehicle, such as aliquid or solid filler, diluent, excipient, solvent or encapsulatingmaterial, involved in maintaining the activity of or carrying ortransporting the subject agents from one organ, or portion of the body,to another organ, or portion of the body. In addition to being“pharmaceutically acceptable” as that term is defined herein, eachcarrier must also be “acceptable” in the sense of being compatible withthe other ingredients of the formulation. The pharmaceutical formulationcontains a compound of the invention in combination with one or morepharmaceutically acceptable ingredients. The carrier can be in the formof a solid, semi-solid or liquid diluent, cream or a capsule. Thesepharmaceutical preparations are a further object of the invention.Usually the amount of active compounds is between 0.1-95% by weight ofthe preparation, preferably between 0.2-20% by weight in preparationsfor parenteral use and preferably between 1 and 50% by weight inpreparations for oral administration. For the clinical use of themethods of the present invention, targeted delivery composition of theinvention is formulated into pharmaceutical compositions orpharmaceutical formulations for parenteral administration, e.g.,intravenous; mucosal, e.g., intranasal; enteral, e.g., oral; topical,e.g., transdermal; ocular, e.g., via corneal scarification or other modeof administration. The pharmaceutical composition contains a compound ofthe invention in combination with one or more pharmaceuticallyacceptable ingredients. The carrier can be in the form of a solid,semi-solid or liquid diluent, cream or a capsule.

The term “oncogene” as used herein refers to a nucleic acid sequenceencoding, or polypeptide, of a mutated and/or overexpressed version of anormal gene that in a dominant fashion can release the cell from normalrestraints on growth and thus alone or in concert with other changes,contribute to a cells tumorigenicity. Examples of oncogenes include;gp40 (v-fms); p21 (ras); p55 (v-myc); p65 (gag-jun); pp60 (v-src);v-abl; v-erb; v-erba; v-fos etc. A proto-oncogene refers to the normalexpression of a nucleic acid expressing the normal, cellular equivalentof an oncogene, typically these genes are usually a gene involved in thesignaling or regulation of cell growth.

The term “regeneration” means regrowth of a cell population, organ ortissue, and in some embodiments after disease or trauma.

The term “vectors” refers to a nucleic acid molecule capable oftransporting another nucleic acid to which it has been linked; a plasmidis a species of the genus encompassed by “vector”. The term “vector”typically refers to a nucleic acid sequence containing an origin ofreplication and other entities necessary for replication and/ormaintenance in a host cell. Vectors capable of directing the expressionof genes and/or nucleic acid sequence to which they are operativelylinked are referred to herein as “expression vectors”. In general,expression vectors of utility are often in the form of “plasmids” whichrefer to circular double stranded DNA loops which, in their vector formare not bound to the chromosome, and typically comprise entities forstable or transient expression or the encoded DNA. Other expressionvectors can be used in the methods as disclosed herein for example, butare not limited to, plasmids, episomes, bacterial artificialchromosomes, yeast artificial chromosomes, bacteriophages or viralvectors, and such vectors can integrate into the host's genome orreplicate autonomously in the particular cell. A vector can be a DNA orRNA vector. Other forms of expression vectors known by those skilled inthe art which serve the equivalent functions can also be used, forexample self replicating extrachromosomal vectors or vectors whichintegrates into a host genome. Preferred vectors are those capable ofautonomous replication and/or expression of nucleic acids to which theyare linked. Vectors capable of directing the expression of genes towhich they are operatively linked are referred to herein as “expressionvectors”. Expression vectors can result in stable or transientexpression of the DNA. An exemplary expression vector for use in thepresent invention is pcDNA3.1.

The term “viral vectors” refers to the use as viruses, orvirus-associated vectors as carriers of the nucleic acid construct intothe cell. Constructs may be integrated and packaged intonon-replicating, defective viral genomes like Adenovirus,Adeno-associated virus (AAV), or Herpes simplex virus (HSV) or others,including reteroviral and lentiviral vectors, for infection ortransduction into cells. The vector may or may not be incorporated intothe cells genome. The constructs may include viral sequences fortransfection, if desired. Alternatively, the construct may beincorporated into vectors capable of episomal replication, e.g., EPV andEBV vectors.

As used herein, a “promoter” or “promoter region” or “promoter element”used interchangeably herein, refers to a segment of a nucleic acidsequence, typically but not limited to DNA or RNA or analogues thereof,that controls the transcription of the nucleic acid sequence to which itis operatively linked. The promoter region includes specific sequencesthat are sufficient for RNA polymerase recognition, binding andtranscription initiation. This portion of the promoter region isreferred to as the promoter. In addition, the promoter region includessequences which modulate this recognition, binding and transcriptioninitiation activity of RNA polymerase. These sequences may be cis-actingor may be responsive to trans-acting factors. Promoters, depending uponthe nature of the regulation may be constitutive or regulated.

The term “regulatory sequences” is used interchangeably with “regulatoryelements” herein refers element to a segment of nucleic acid, typicallybut not limited to DNA or RNA or analogues thereof, that modulates thetranscription of the nucleic acid sequence to which it is operativelylinked, and thus act as transcriptional modulators. Regulatory sequencesmodulate the expression of gene and/or nucleic acid sequence to whichthey are operatively linked. Regulatory sequence often comprise“regulatory elements” which are nucleic acid sequences that aretranscription binding domains and are recognized by the nucleicacid-binding domains of transcriptional proteins and/or transcriptionfactors, repressors or enhancers etc. Typical regulatory sequencesinclude, but are not limited to, transcriptional promoters, induciblepromoters and transcriptional elements, an optional operate sequence tocontrol transcription, a sequence encoding suitable mRNA ribosomalbinding sites, and sequences to control the termination of transcriptionand/or translation. Regulatory sequences can be a single regulatorysequence or multiple regulatory sequences, or modified regulatorysequences or fragments thereof. Modified regulatory sequences areregulatory sequences where the nucleic acid sequence has been changed ormodified by some means, for example, but not limited to, mutation,methylation etc.

The term “operatively linked” as used herein refers to the functionalrelationship of the nucleic acid sequences with regulatory sequences ofnucleotides, such as promoters, enhancers, transcriptional andtranslational stop sites, and other signal sequences. For example,operative linkage of nucleic acid sequences, typically DNA, to aregulatory sequence or promoter region refers to the physical andfunctional relationship between the DNA and the regulatory sequence orpromoter such that the transcription of such DNA is initiated from theregulatory sequence or promoter, by an RNA polymerase that specificallyrecognizes, binds and transcribes the DNA. In order to optimizeexpression and/or in vitro transcription, it may be necessary to modifythe regulatory sequence for the expression of the nucleic acid or DNA inthe cell type for which it is expressed. The desirability of, or needof, such modification may be empirically determined Enhancers need notbe located in close proximity to the coding sequences whosetranscription they enhance. Furthermore, a gene transcribed from apromoter regulated in trans by a factor transcribed by a second promotermay be said to be operatively linked to the second promoter. In such acase, transcription of the first gene is said to be operatively linkedto the first promoter and is also said to be operatively linked to thesecond promoter.

As used herein, the term “biological sample” also refers to a cell orpopulation of cells or a quantity of tissue or fluid from a subject.Most often, the sample has been removed from a subject, but the term“biological sample” can also refer to cells or tissue analyzed in vivo,i.e. without removal from the subject. Often, a “biological sample” willcontain cells from a subject, but the term can also refer tonon-cellular biological material, such as non-cellular fractions ofblood, saliva, or urine, that can be used to measure proteinphosphorylation levels. In some embodiments, a “biological sample” or“tissue sample” refers to a sample of tissue or fluid isolated from anindividual, including but not limited to, for example, blood, plasma,serum, tumor biopsy, urine, stool, sputum, spinal fluid, pleural fluid,nipple aspirates, lymph fluid, the external sections of the skin,respiratory, intestinal, and genitourinary tracts, tears, saliva, milk,cells (including but not limited to blood cells), tumors, organs, andalso samples of in vitro cell culture constituent. In some embodiments,a biological sample is from a resection, bronchoscopic biopsy, or coreneedle biopsy of a primary, secondary or metastatic tumor, or acellblock from pleural fluid. In addition, fine needle aspiratebiological samples are also useful. In some embodiments, a biologicalsample is primary ascite cells. Samples can be fresh, frozen, fixed oroptionally paraffin-embedded, frozen or subjected to other tissuepreservation methods, including for example methods to preserve thephosphorylation status of polypeptides in the biological sample. Abiological sample can also mean a sample of biological tissue or fluidthat comprises protein or cells. Such samples include, but are notlimited to, tissue isolated from subjects or animals. Biological samplesmay also include sections of tissues such as biopsy and autopsy samples,frozen sections taken for histological purposes, blood, plasma, serum,sputum, stool, tears, mucus, hair, and skin. Biological samples alsoinclude explants and primary and/or transformed cell cultures derivedfrom patient tissues. A biological sample may be provided by removing asample of cells from subject, but can also be accomplished by usingpreviously isolated cells (e.g., isolated by another person, at anothertime, and/or for another purpose), or by performing the methods of theinvention in vivo. Archival tissues, such as those having treatment oroutcome history may also be used. Biological samples include, but arenot limited to, tissue biopsies, scrapes (e.g. buccal scrapes), wholeblood, plasma, serum, urine, saliva, cell culture, or cerebrospinalfluid. Biological samples also include tissue biopsies, cell culture.The biological sample can be obtained by removing a sample of cells froma subject, but can also be accomplished by using previously isolatedcells (e.g. isolated by another person), or by performing the methods ofthe invention in vivo. Such samples include, but are not limited to,whole blood, cultured cells, primary cell preparations, sputum, amnioticfluid, tissue or fine needle biopsy samples, peritoneal fluid, andpleural fluid, among others. In some embodiments a biological sample istaken from a human patient, and in alternative embodiments thebiological sample is taken from any mammal, such as rodents, animalmodels of diseases, commercial animals, companion animals, dogs, cats,sheep, cattle, and pigs, etc. The biological sample can be pretreated asnecessary for storage or preservation, by dilution in an appropriatebuffer solution or concentrated, if desired. Any of a number of standardaqueous buffer solutions, employing one of a variety of buffers, such asphosphate, Tris, or the like, at physiological pH can be used. Thebiological sample can in certain circumstances be stored for use priorto use in the assay as disclosed herein. Such storage can be at +4 C orfrozen, for example at −20 C or −80 C, provided suitablecryopreservation agents are used to maintain cell viability once thecells are thawed.

The term “reduced” or “reduce” or “decrease” or “lower” as used hereingenerally means a decrease by a statistically significant amountrelative to a reference. However, for avoidance of doubt, “reduced”means statistically significant decrease of at least 10% as compared toa reference level, for example a decrease by at least 20%, at least 30%,at least 40%, at least t 50%, or least 60%, or least 70%, or least 80%,at least 90% or more, up to and including a 100% decrease (i.e. absentlevel as compared to a reference sample), or any decrease between10-100% as compared to a reference level, as that term is definedherein. The term “decrease” or “inhibition” used in the context of thelevel of expression or activity of a gene refers to a reduction inprotein or nucleic acid level or activity in a cell, a cell extract, ora cell supernatant. For example, such a decrease may be due to reducedRNA stability, transcription, or translation, increased proteindegradation, or RNA interference. Preferably, this decrease is at leastabout 5%, at least about 10%, at least about 25%, at least about 50%, atleast about 75%, at least about 80%, or even at least about 90% of thelevel of expression or activity under control conditions.

The term “low” as used herein generally means lower by a staticallysignificant amount; for the avoidance of doubt, “low” means astatistically significant value at least 10% lower than a referencelevel, for example a value at least 20% lower than a reference level, atleast 30% lower than a reference level, at least 40% lower than areference level, at least 50% lower than a reference level, at least 60%lower than a reference level, at least 70% lower than a reference level,at least 80% lower than a reference level, at least 90% lower than areference level, up to and including 100% lower than a reference level(i.e. absent level as compared to a reference sample).

The terms “increased” or “increase” as used herein generally mean anincrease by a statically significant amount; for the avoidance of doubt,“increased” means a statistically significant increase of at least 10%as compared to a reference level, including an increase of at least 20%,at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, at least 100% or more, including, for exampleat least 2-fold, at least 3-fold, at least 4-fold, at least 5-fold, atleast 10-fold increase or greater as compared to a reference level, asthat term is defined herein. The term an “increase” as used in thecontext of the expression or activity of a gene or protein is meant apositive change in protein or nucleic acid level or activity in a cell,a cell extract, or a cell supernatant. For example, such a increase maybe due to increased RNA stability, transcription, or translation, ordecreased protein degradation. Preferably, this increase is at least 5%,at least about 10%, at least about 25%, at least about 50%, at leastabout 75%, at least about 80%, at least about 100%, at least about 200%,or even about 500% or more over the level of expression or activityunder control conditions.

The term “high” as used herein generally means a higher by a staticallysignificant amount relative to a reference; for the avoidance of doubt,“high” means a statistically significant value at least 10% higher thana reference level, for example at least 20% higher, at least 30% higher,at least 40% higher, at least 50% higher, at least 60% higher, at least70% higher, at least 80% higher, at least 90% higher, at least 100%higher, at least 2-fold higher, at least 3-fold higher, at least 4-foldhigher, at least 5-fold higher, at least 10-fold higher or more, ascompared to a reference level.

The articles “a” and “an” are used herein to refer to one or to morethan one (i.e., to at least one) of the grammatical object of thearticle. By way of example, “an element” means one element or more thanone element.

Other than in the operating examples, or where otherwise indicated, allnumbers expressing quantities of ingredients or reaction conditions usedherein should be understood as modified in all instances by the term“about.” The term “about” when used in connection with percentages canmean±1%. The present invention is further explained in detail by thefollowing examples, but the scope of the invention should not be limitedthereto.

It should be understood that this invention is not limited to theparticular methodology, protocols, and reagents, etc., described hereinand as such can vary. The terminology used herein is for the purpose ofdescribing particular embodiments only, and is not intended to limit thescope of the present invention, which is defined solely by the claims.Other features and advantages of the invention will be apparent from thefollowing Detailed Description, the drawings, and the claims.

Mullerian Inhibiting Substance (MIS)

Without wishing to be bound by theory, the Mullerian InhibitingSubstance (MIS) is a member of the TGFβ multigene family ofglycoproteins. The proteins in this gene family are all produced asdimeric precursors and undergo posttranslational processing foractivation, requiring cleavage and dissociation to release bioactiveC-terminal fragments. MIS is a 140-kDa dimer which consists of identical70 kDa disulfide-linked monomers, each composed of a 57 kDa N-terminaldomain and a 12.5 kDa carboxyl-terminal (C-terminal). Thus, MIScomprises 2 identical monomers (and thus is termed a “homodimer”), eachmonomer comprising two domains, the N-terminal and C-terminal domain,which are held in non-covalent association. The purified C-terminaldomain is the biologically active moiety and cleavage is required foractivity. The N-terminal domain may assist with protein folding in vivoand facilitate delivery of the C-terminal peptide to its receptor, e.g.,MISRI and MISRII. A non-cleavable mutant of MIS is biologicallyinactive.

The carboxy-terminal active domain shares amino acid homology with otherTGFb family members, such as TGF-B 1, 2, and 3, inhibin, activin, andbone morphogenetic proteins, as well as a member of Growth andDifferentiation Factors (GDFs). The structure of the MIScarboxy-terminal domain is supported by seven cysteines involved both inintra- and intermolecular disulfides bridges that lead to its structuralstability, as revealed by homology to the three dimensional structure ofTGFb using molecular modeling (Lorenzo, Donahoe, et al., unpublisheddata).

Like other TGFb family members, MIS can be cleaved by plasmin whichgenerates its amino- and carboxy-terminal domains. This proteolyticprocess is required for its physiological activity and occurs at a sitein a position similar to the dibasic cleavage site found in the sequenceof TGFb. The resultant products are tightly associated in a non-covalentcomplex that dissociates at low pH; therefore, technically complex andtime-demanding protocols with plasmin treatment and molecular sizeexclusion chromatography are required to enhance or complete theseparation of the carboxy terminus from the amino terminus.

MIS contains two major cleavage sites that are sensitive to plasmin; theprimary monobasic site which is located at amino acid position 426-427of human wild-type MIS (corresponding to amino acid 451-452 of SEQ IDNO:1 herein). Cleavage at this site, which releases the activecarboxy-terminal domain of MIS, resembles a consensus furin cleavagesite. A secondary cleavage site (referred to as “12/S”), identified byamino-terminal sequencing of MIS fragments is located at residues229-230 in the amino-terminal domain of wild-type MIS (corresponding toamino acids 254-255 of SEQ ID NO: 1). This site contains an R/S, butotherwise does not follow the consensus Arg-X-(Arg/Lys)-Arg for furincleavage. Separation of purified carboxy-terminal from amino-terminalMIS after digestion with exogenous plasmin previously used molecularsize-exclusion chromatography under acidic conditions. This techniquerequires extreme care to control MIS digestion, since long incubationsof MIS in plasmin produced the carboxy-terminal MIS domain plus otherfragments of 22 and 34 kDa, due to cleavage both at the primary andsecondary sites, are extremely difficult to separate from one another bysize exclusion. Since all fragments generated after plasmin digestionare glycosylated, except the carboxy-terminal domain, wheat-germ lectinaffinity can be used as an alternative to size chromatography separationto purify the carboxy-terminal domain of MIS. After plasmin cleavage,the resulting fragments can be loaded onto a wheat germ lectin column atpH 3.5 in order to dissociate the amino- and carboxy-terminal domains,as disclosed in Lorenzo et al., J. Chromatography, (2001), 776; 89-98,which is incorporated herein its entirety by reference.

Accordingly, to overcome several issues with respect to avoiding theproduction of fragments of MIS, e.g., both the carboxy-terminal MISdomain plus a 22 and 34 kDa fragments due to cleavage both at theprimary and secondary sites, the inventors have modified the primarycleavage site at amino acid position 426-427 of human wild-type MIS(corresponding to amino acid 451-452 of SEQ ID NO:1 herein). To aid thepurification of the C-terminal domain without the need for complicatedmethods using wheat-germ lectin affinity or size chromatography columns,the most flexible domain of the C-terminus, the inventors have includeda tag at the N-terminus of the C-terminal domain.

Furthermore, the wild-type MIS protein is produced as a prohormonecomprising a N-terminal leader sequence, which corresponds to amino acidresidues 1-25 of SEQ ID NO: 1. Processing of the mature hormone MISprotein can involve the proteolytic cleavage and removal of the leadersequence (e.g., amino acids 1-25 of SEQ ID NO: 1), the cleavage of theMIS protein at the primary site to generate the N-terminal andC-terminal domains, and the formation of these domains into a monomer,which is disulfide linked by inter- and intrachain disulfide bonds to anidentical monomer to form the bioactive homodimer MIS protein.

Leader Sequences

Without wishing to be bound by theory, leader sequences improveexpression and/or secretion of a polypeptide of interest in a host cell,and are useful for the recombinant production of proteins. Generally, asan efficient method for secreting a desired protein by a geneticengineering procedure, a method is known wherein a fused proteincomprising the desired protein (e.g., MIS) and a prepropeptide (signalpeptide+propeptide) is expressed in a host cell and then intracellularlycleaved (processed) by enzymes of the host, and then, extracellularlysecreted. According to this process, however, the fused protein must becleaved twice by enzymes of the host to be a mature protein, resultingin lower yield of the mature protein and contamination of the matureprotein with residual fused protein.

Accordingly, secreted proteins are expressed initially inside the cellin a precursor form containing a leader sequence ensuring entry into thesecretory pathway. Such leader sequences, also referred to as signalpeptides, direct the expressed product across the membrane of theendoplasmic reticulum (ER). Signal peptides are generally cleaved off bysignal peptidases during translocation to the ER. Once entered in thesecretory pathway, the protein is transported to the Golgi apparatus.From the Golgi the protein can follow different routes that lead tocompartments such as the cell vacuole or the cell membrane, or it can berouted out of the cell to be secreted to the external medium (Pfefferand Rothman (1987) Ann. Rev. Biochem. 56:829-852).

For Industrial production of a secreted protein, the protein to beproduced needs to be secreted efficiently from the host cell or the hostorganism. The signal peptide may be, e.g., the native signal peptide ofthe protein to be produced, a heterologous signal peptide, or a hybridof native and heterologous signal peptide. However, several problems areencountered with the use of currently known signal peptides. One problemoften encountered when producing a human protein from a non-human hostcell or organism is that the native signal peptide does not ensureefficient translocation and/or cleavage of the signal peptide. Thisleads to low rates of protein secretion and/or to secretion of matureproteins that display N-terminal extensions due to an incorrect cleavageof the signal peptide. Thus the choice of the signal peptide is of greatimportance for industrial production of a protein.

In addition of leader sequences directing the secretion of the protein,a precursor form can comprise supplemental leader sequences that arecleaved during maturation. These supplemental leader peptides, namedpropeptides, usually follow the signal peptide. Virtually all peptidehormones, numerous bioactive protein (for example, growth factors,receptors and cell-adhesion molecules, and including MIS), and manybacterial toxins and viral envelope glycoproteins comprise a propeptidethat is post-translationally excised to generate the mature andbiologically active protein (Seidah and Chretien (1999) Brain Res.848:45-62).

Peptides are further cleaved by enzymes named proprotein convertases.Mammalian proprotein convertases include, e.g., the subtilisinconvertases PCSK1, PCSK2 and furin. Furin is ubiquitously expressed andlocated in the trans-Golgi network. Furin proteolytically activateslarge numbers of proproteins substrates in secretory pathwaycompartments. (Thomas (2002) Nat Rev Mol Cell Biol. 3:753-766). Morespecifically, furin localizes to the Trans Golgi Network, a late Golgistructure that is responsible for sorting secretory pathway proteins totheir final destinations, including the cell surface, endosomes,lysosomes and secretory granules. The site that furin cleaves has beenextensively studied. The cleavage site is positioned after thecarboxyl-terminal arginine of the consensus sequence R-X-L/R-R, whereinX may represent any amino acid (Nakayama (1997) Biochem. J 327:625-635).The cleavage efficiency is increased when X is a lysine, a valine, anisoleucine or an alanine (Watanabe et al (1992) J Biol. Chem.267:8270-8274).

In some embodiments, the recombinant human MIS protein comprises amodified leader sequence in place of the wild-type leader sequence ofthe MIS protein of SEQ ID NO:1. In some embodiments, the native leadersequence of amino acid residues 1-25 of SEQ ID NO: 1 is replaced with anon-MIS leader sequence, for example, but not limited to an albuminleader sequence, or functional fragment thereof. In some embodiments,the non-MIS leader sequence is a human serum albumin sequence (HSA), forexample, a leader sequence corresponding to SEQ ID NO:6, which isencoded by nucleic acids corresponding to SEQ ID NO: 7.

In some embodiments, a HSA sequence is a functional fragment of SEQ IDNO: 6, for example, or at least 23, or at least 22, or at least 21, orat least 20, or at least 19, or at least 18, or at least 17, or at least16, or at least 15, or at least 14, or at least 13, or at least 12, orat least 11, or at least 10, or less than 10 consecutive ornon-consecutive amino acids of SEQ ID NO:6. Modified versions of HSAleader sequence are also encompassed for use in the present inventionand are disclosed in U.S. Pat. No. 5,759,802 which is incorporatedherein in its entirety by reference. In some embodiments, a functionalfragment of HSA leader sequence is MKWVTFISLLFLFSSAYS (SEQ ID NO: 13) orvariations therefor, which are disclosed in EP patent EP2277889 which isincorporated herein in its entirety. Variants of the pre-pro region ofthe HSA signal sequence (e.g., MKWVTFISLLFLFSSAYSRGVFRR, SEQ ID NO: 6)include fragments, such as the pre region of the HSA signal sequence(e.g., MKWVTFISLLFLFSSAYS, SEQ ID NO:13) or variants thereof, such as,for example, MKWVSFISLLFLFSSAYS, (SEQ ID NO:14).

In some embodiments, the leader sequence is a leader sequence is atleast about 60%, or at least about 70%, or at least about 80%, or atleast about 90%, or at least about 95%, or at least about 96%, or atleast about 97%, or at least about 98%, or at least about 99% identicalto amino acid residues of SEQ ID NO: 6.

The HSA leader sequence as used herein resulted in an expected increasedyield (both higher concentration and higher production) of therecombinant human MIS protein (see FIGS. 2 and 3). However, the presenceof the HSA leader sequence also resulted in a surprising and unexpectedincrease in cleavage from the primary cleavage site (corresponding tocleavage at 451/452 of SEQ ID NO: 1 (or 426/427 of conventional aminoacid nomenclature of wild-type human MIS protein) (see FIGS. 2 and 3).This increased yield and increased cleavage was surprising because withan increased yield (and therefore more protein produced by the cell),one would expect a decreased cleavage as the activity of the availablecleavage enzymes becomes saturated and overextended. However, this wasnot the case—in fact the exact opposite occurred where with increasedprotein production there was increased cleavage from the primarycleavage site.

Other leader sequences are encompassed for use in a recombinant humanMIS protein as disclosed herein, e.g., to replace amino acids 1-25 ofSEQ ID NO: 1. Such leader sequences are well known in the art, andinclude the leader sequences comprising an immunoglobulin signal peptidefused to a tissue-type plasminogen activator propeptide (IgSP-tPA), asdisclosed in US 2007/0141666, which is incorporated herein in itsentirety by reference. Numerous other signal peptides are used forproduction of secreted proteins. One of them is a murine immunoglobulinsignal peptide (IgSP, EMBL Accession No. M13331). IgSP was firstidentified in 1983 by Loh et al. (Cell. 33:85-93). IgSP is known to givea good expression in mammalian cells. For example. EP patent No. 0382762discloses a method of producing horseradish peroxidase by constructing afusion polypeptide between IgSP and horseradish peroxidase.

Other leader sequences include, for example, but not limited to, theMPIF-1 signal sequence (e.g., amino acids 1-21 of GenBank Accessionnumber AAB51134) MKVSVAALSCLMLVTALGSQA (SEQ ID NO: 15); thestanniocalcin signal sequence (MLQNSAVLLLLVISASA, SEQ ID NO:16); theinvertase signal sequence (e.g., MLLQAFLFLLAGFAAKISA, SEQ ID NO:17); theyeast mating factor alpha signal sequence (e.g., K. lactis killer toxinleader sequence); a hybrid signal sequence (e.g.,MKWVSFISLLFLFSSAYSRSLEKR, SEQ ID NO:18); an HSA/MFα-1 hybrid signalsequence (also known as HSA/kex2) (e.g., MKWVSFISLLFLFSSAYSRSLDKR, SEQID NO:19); a K. lactis killer/MFα-1 fusion leader sequence (e.g.,MNIFYIFLFLLSFVQGSLDKR, SEQ ID NO:20); the Immunoglobulin Ig signalsequence (e.g., MGWSCIILFLVATATGVHS, SEQ ID NO:21); the Fibulin Bprecursor signal sequence (e.g., MERAAPSRRVPLPLLLLGGLALLAAGVDA, SEQ IDNO:22); the clusterin precursor signal sequence (e.g.,MMKTLLLFVGLLLTWESGQVLG, SEQ ID NO: 23); and the insulin-like growthfactor-binding protein 4 signal sequence (e.g., MLPLCLVAALLLAAGPGPSLG,SEQ ID NO:24).

Where it is desirable to produce recombinant MIS in a bacterial system,leader sequences can include bacterial leader sequences as disclosed inUS Application 2011/0020868. A number of other secretion signals havebeen described for use in expressing recombinant polypeptides orproteins. See, for example, U.S. Pat. Nos. 5,914,254; 4,963,495;European Patent No. 0 177 343; U.S. Pat. No. 5,082,783; PCT PublicationNo. WO 89/10971; U.S. Pat. Nos. 6,156,552; 6,495,357; 6,509,181;6,524,827; 6,528,298; 6,558,939; 6,608,018; 6,617,143; 5,595,898;5,698,435; and 6,204,023; 6,258,560; PCT Publication Nos. WO 01/21662,WO 02/068660 and U.S. Application Publication 2003/0044906; U.S. Pat.No. 5,641,671; and European Patent No. EP 0 121 352, which areincorporated herein in their entirety by reference.

Modified Cleavage Sites

As discussed herein, the preparation of a MIS protein for preclinicaluse is complex and inefficient. Human MIS protein is produced from apre-proprotein, which comprises a leader sequence. The leader sequence(amino acids 1-25 of SEQ ID NO: 1) is cleaved off and the remainingprotein (often called “holo-human MIS”), and corresponding to amino acidresidues 26-560 of SEQ ID NO:1, must be additionallypost-translationally cleaved to result in an N-terminal and anC-terminal domain. These N-terminal and C-terminal domains form amonomer, and two identical monomers (comprising the N- and C-terminaldomains) form together to generate a homodimer. Holo-human MIS iscleaved into its N- and C-terminal domains most likely by means of furinor a related prohormone convertase PCS, expressed in the gonads.Cleavage occurs primarily at a kex-like site characterized by R⁻⁴ XXR⁻¹with a serine in the +1 site, which makes the MIS cleavage sitemonobasic, but more furin/hex consensus. The purified C-terminal domainis the biologically active moiety and cleavage is required forbiological activity. A secondary cleavage site, whose significance isunknown, is observed less frequently at residues 229-230 (whichcorrespond to amino acids 254-255 of SEQ ID NO: 1). Non-cleavablemutants of MIS are not biologically active and mutations in the humangene that truncate the carboxy-terminal domain lead to persistentMullerian duct syndrome. The cleavage of recombinantly expressed MISprotein by CHO cells is incomplete and inefficient, thus cleavage withan exogenous serine protease such as plasmin is required to enhancebioactivity.

Herein, the inventors have modified the kex-like site characterized byR⁻⁴ XXR⁻¹ with an R in the −2 site, which makes the monobasic MIScleavage site more like a consensus Kex/Furin recognition site. Inparticular, in one embodiment, the recombinant human MIS is producedfrom a proprotein where the amino acid residue at position 450 of SEQ IDNO: 1 has been changed from a Q (glutamine or Gln) to a R (arginine, orArg). This mutation is can be referred to as Q450R of SEQ ID NO:1. Thiscorresponds to a change in amino acid residue 425 (Q425R) of MIS whichis numbered with conventional protein numbering, where the firstnumbered amino acid begins after the leader sequence.

This change in amino acid sequence of Q450R of SEQ ID NO:1 allows forproduction of a highly purified cleaved preparation of human MIS proteinwhich has full bioactivity.

In alternative embodiments, the primary cleavage site in the MISprotein, e.g., the monobasic site which is located at amino acidposition 426-427 of human wild-type MIS (corresponding to amino acid451-452 of SEQ ID NO:1 herein) can be modified to an amino acidrecognition site which is recognized by a different cleavage enzyme. Forexample, the primary cleavage site in the MIS protein, e.g., themonobasic site which is located at amino acid position 426-427 can bemodified to an amino acid sequence which is recognized by a protease orpeptidase, such as pro-hormone convertases (PC's), or other cleavingagents expressed by a cell and found in surrounding tissue, or producedby a microbe capable of establishing an infection in a mammal.Enzyme-cleavable peptides can, but are not required to, contain one ormore amino acids in addition to the amino acid recognition sequence;additional amino acids can be added to the amino terminal, carboxyterminal, or both the amino and carboxy terminal ends of the recognitionsequence. Means of adding amino, acids to an amino acid sequence, e.g.,in an automated peptide synthesizer, as well as means of detectingcleavage of a peptide, e.g., by chromatographic analysis for the aminoacid products of such cleavage, are well known to ordinarily skilledartisans given the teachings of this invention.

Prohormone protein convertases constitute a family of serine proteasesstructurally related to bacterial subtilisins and to yeast kexin.Several eukaryotic members of this family are currently known.Pro-hormone Convertases (PC's) cleave precursor polypeptides at specificbasic residues, most often after selected paired basic residues, togenerate bioactive peptide and proteins. Many members of the insulinfamily of proteins (e.g. Insulin, Igf-1) are substrates for PC's.

Tags to Enhance Purification

In some embodiments, a recombinant MIS protein comprises at least oneinternal label or “tag”. In some embodiments the tag can be, forexample, a c-myc, poly histidine, or FLAG tag. In some embodiments, thetag is a FLAG tag, for example, a FLAG tag of SEQ ID NO:8. A FLAG tagcan be encoded by the nucleic acid of SEQ ID NO 9.

In some embodiments, the tag on the recombinant human MIS protein isinternal at the carboxy terminus immediately downstream from thecleavage site. As it is the most flexible part of the C-terminus and notinvolved in binding to receptor and rendering specificity, as are the“fingertips” of the C-terminus (Papakostas et al, 2010, Lorenzo et al,2002). In some embodiments, the labeling at this site is most likely topreserve biologic activity. In some embodiments, a tag, e.g., a FLAG tagis located after the primary cleavage site, e.g., after amino acid 452of SEQ ID NO: 1 (corresponding to amino acid residue 427 of conventionalprotein nomenclature). In some embodiments, a tag is located betweenamino acid residues 452 and 453 of SEQ ID NO: 1 (which corresponds withamino acid residues 427 and 428 under normal amino acid nomenclature ofMIS protein).

In alternative embodiments, the tag or label is located at any positionbetween sequence 450 and 560 of SEQ ID NO: 1. In some embodiments, thetag is inserted 2 amino acid residues after the modified amino acid atposition 450 of SEQ ID NO: 1. However, a position of the tag at theN-terminus of the C-terminal domain of MIS is preferred, as it locationat the C-terminus of the C-terminal domain renders the C-terminal domaintotally inactive, significantly reducing the bioactivity of the MISprotein.

In some embodiments, a recombinant MIS protein comprises more than onetag, e.g., for example, at least 2 or at least 3, or at least 4 or morethan 4 tags. In some embodiments, the tags are sequential (e.g., oneafter another) and in some embodiments, they are dispersed (e.g.,intermittent) in the recombinant human MIS protein. Preferably, the tagsdo not interfere or substantially affect the bioactivity of therecombinant MIS protein function at binding and activating MISRII. Insome embodiments, where the recombinant MIS protein comprises more thanone tag, the tags are the same tag. In alternative embodiments, wherethe recombinant MIS protein comprises more than one tag, the tags aredifferent tags, for example, a recombinant MIS protein can comprise aFLAG tag and a histidine tag. The small size of the Flag tag allows itto be contained in the flexible, non binding N-terminal domain of theC-terminus. Accordingly, in some embodiments, any tag known to a personof ordinary skill in the art can be used in place of the Flag Tag, forexample a tag of between about 5-10 amino acids, or between about 10-15amino acids, or a tag between about 15-20 amino acids, or a tag between20-30 amino acids, or a tag between about 30-50 amino acids. In someembodiments, a tag greater than 50 amino acids in length is notrecommended, as the tag may sterically hinder the flexible N-terminus ofthe C-terminal domain, and thus inhibit the bioactivity of therecombinant MIS protein.

In some embodiments, a tag-labeled, e.g., FLAG tagged recombinant humanMIS protein, such as the LRF recombinant human MIS protein as disclosedherein (see FIG. 1) can be eluted by a single step to produce highlypurified efficiently cleaved preparation with full bioactivity. Whenscaled-up, this purification of recombinant human MIS protein will besuitable for clinical applications; furthermore it will be useful forvarious binding assays in both clinical and experimental settings.Internal labeling of MIS during translation has proved to be moreeffective than labeling after purification of the protein as iodinationor biotinylation greatly reduced MIS bioactivity. Surprisingly, theinventors have discovered that the LRF recombinant human MIS proteinconstruct is more bioactive than the wild-type MIS. Inserting the FLAGtag sequence has several other distinct advantages. First, its uniqueamino acid domain is not present in any other gene (except for mousebrain phosphatase), thus making the anti-FLAG antibody very specific.Second, the elution of the protein with the 3× FLAG peptide is specificfor the FLAG MIS and not other proteins that bind non-specifically tothe agarose beads.

Surprisingly, a FLAG-tagged, cleavage optimized recombinant human MIS(e.g., a RF recombinant human MIS or RARR/S (SEQ ID NO: 27) FLAG MIS)was bioactive whereas a FLAG-tagged, non-cleavage optimized recombinanthuman MIS (e.g., RAQR/R (SEQ ID NO: 28) FLAG MIS) was not when comparedto native human MIS or to the previously prepared untagged RAQR/R (SEQID NO: 28) MIS. As it is likely that the presence of the acidic FLAG tagso close to the cleavage site may impair the degree of cleavage, thuscausing loss of activity. Thus, the inventors did not anticipateenhanced cleavage with the addition of the Flag tag. Moreover, the holoRAQR/RFLAG MIS (“RAQR/R” disclosed as SEQ ID NO: 28) preparation in CHO(or HEK) cells is not bioactive, as no endogenous processing occurs withthe RAQR/R (SEQ ID NO: 28) cleavage site in contrast to what wasreported by Kurian (Cancer Res., 1995. 1; 343-349) when the constructlacked the FLAG tag. On the other hand, the retention of the serine atposition 428 and the conversion of the monobasic site to dibasic(corresponding to Q>R at amino acid position 425 using conventionalprotein nomenclature), or Q>R at position 450 of SEQ ID NO: 1) makes theendogenous cleavage more efficient and very specific. Furthermore, a tagsuch as a FLAG MIS is a powerful tool for binding studies, and can beused to immunoprecipitate the endogenous MISRII without crosslinking.Accordingly, in some embodiments, a labeled recombinant human MISprotein, e.g., a MIS with an internal FLAG is useful in an efficientmethod for producing a highly pure and biologically active internallylabeled form of MIS, which can be used for scale-up for preclinical andclinical use, for the study of MIS binding proteins and for tracking inpharmacokinetic studies.

Variants of a Human Recombinant MIS Protein.

In some embodiments, a recombinant human MIS protein as disclosed canhave a modification in the core MIS protein sequence, e.g., amino acidsresidues 26-560 of SEQ ID NO: 1 (including a modification of amino acidresidue 450 from Q to R of SEQ ID NO: 1) and/or the insertion of a tagat the beginning of the C-terminal domain). Such variants are consideredto be homologous to wild-type MIS protein.

As used herein, the term “polypeptide” refers to a polymer of aminoacids and its equivalent and does not refer to a specific length of theproduct; thus, peptides, oligopeptides and proteins are included withinthe definition of a polypeptide. A derivative is a polypeptide havingconservative amino acid substitutions, as compared with anothersequence. Derivatives further include other modifications of proteins,including, for example, modifications such as glycosylations,acetylations, phosphorylations, and the like.

In some embodiments, a recombinant human MIS protein is at least 75%, atleast 80%, at least 85%, at least 90% or at least 95% similar to thehomologous recombinant human MIS protein. As used herein, “similarity”or “percent similarity” in the context of two or polypeptide sequences,refer to two or more sequences or subsequences that are the same or havea specified percentage of amino acid residues or conservativesubstitutions thereof, that are the same, when compared and aligned formaximum correspondence, as measured using one of the following sequencecomparison algorithms, or by visual inspection. By way of example, afirst amino acid sequence can be considered similar to a second aminoacid sequence when the first amino acid sequence is at least 50%, 60%,70%, 75%, 80%, 90%, or even 95% identical, or conservativelysubstituted, to the second amino acid sequence when compared to an equalnumber of amino acids as the number contained in the first sequence, orwhen compared to an alignment of polypeptides that has been aligned by acomputer similarity program known in the art, as discussed below.

Homologues and functional derivatives and functional fragments of MIS ofSEQ ID NO: 1 are also encompassed for use in the present invention, andcan also be identified, for example, by expression of MIS from anexpression library. (See, e.g., Sambrook et al. (2001). Molecularcloning: a laboratory manual, 3rd ed. (Cold Spring Harbor, N.Y., ColdSpring Harbor Laboratory Press); Ausubel et al., supra.) A mutatedendogenous gene sequence can be referred to as a heterologous transgene;for example, a transgene encoding a mutation in MIS which is not knownin naturally-occurring genomes is a heterologous transgene with respectto murine and non-murine, e.g., human species. A MIS protein, such as,for example, those disclosed in U.S. Pat. Nos. 5,427,780, 5,359,033 and5,661,126 (the disclosures of which are incorporated by referenceherein).

The variation in primary structure of core human MIS protein sequence(e.g., amino acids residues 26-560 of SEQ ID NO: 1 (including amodification of amino acid residue 450 from Q to R of SEQ ID NO: 1)and/or the insertion of a tag at the beginning of the N-terminal domainof the C-terminal domain), or functional fragment, or a homologue areencompassed for use in the present invention, for instance, may includedeletions, additions and substitutions. The substitutions may beconservative or non-conservative. The differences between a recombinanthuman MIS protein and a variant generally conserve desired properties,mitigate or eliminate undesired properties and add desired or newproperties. For example, variants of a recombinant human MIS protein canhave superior activity as compared to wild-type MIS protein.

It will be appreciated by those of skill that the core human MIS proteinsequence (e.g., amino acids residues 26-560 of SEQ ID NO: 1) of arecombinant human MIS protein as disclosed herein can be readilymanipulated to alter the amino acid sequence of a protein. A geneencoding the MIS protein or a functional fragment, homologue or variantthereof, can be manipulated by a variety of well known techniques for invitro mutagenesis, among others, to produce variants of the naturallyoccurring human protein or fragment thereof, herein referred to asvariants or muteins, may be used in accordance with the invention.

Other Modifications to a Recombinant Human MIS Protein

The recombinant human MIS protein useful in the present invention canalso be modified at their amino termini, for example, so as to increasetheir hydrophilicity. Increased hydrophobicity enhances exposure of thepeptides on the surfaces of lipid-based carriers into which the parentpeptide-lipid conjugates have been incorporated. Polar groups suitablefor attachment to peptides so as to increase their hydrophilicity arewell known, and include, for example and without limitation: acetyl(“Ac”), 3-cyclohexylalanyl (“Cha”), acetyl-serine (“Ac Ser”),acetyl-seryl-serine (“Ac-Ser-Ser-”), succinyl (“Suc”), succinyl-serine(“Suc-Ser”), succinyl-seryl-serine (“Suc-Ser-Ser”), methoxy succinyl(“MeO-Suc”), methoxy succinyl-serine (“MeO-Suc-Ser”), methoxysuccinyl-seryl-serine (“MeO-Suc-Ser-Ser”) and seryl-serine (“Ser-Ser-”)groups, polyethylene glycol (“PEG”), polyacrylamide,polyacrylomorpholine, polyvinylpyrrolidine, a polyhydroxyl group andcarboxy sugars, e.g., lactobionic, N-acetyl neuraminic and sialic acids,groups. The carboxy groups of these sugars would be linked to theN-terminus of the peptide via an amide linkage. Presently, the preferredN-terminal modification is a methoxy-succinyl modification.

In some embodiments, a recombinant human MIS protein can be fused to oneor more fusion partners. In certain embodiments, one of the fusionpartners is the Fc protein (e.g., mouse Fc or human Fc). The fusionprotein may further include a second fusion partner such as apurification or detection tag, for example, proteins that may bedetected directly or indirectly such as green fluorescent protein,hemagglutinin, or alkaline phosphatase), DNA binding domains (forexample, GAL4 or LexA), gene activation domains (for example, GAL4 orVP16), purification tags, or secretion signal peptides (e.g.,preprotyrypsin signal sequence).

In one embodiment, a recombinant human MIS protein fusion protein usefulin the methods and compositions as disclosed herein can comprise a humanFc protein or a functional fragment thereof. Accordingly, in oneembodiment, a recombinant human MIS protein fusion protein useful in themethods and compositions as disclosed herein can comprises a human Fcmolecule as the first fusion partner, where the Fc fragment can be SEQID NO: 10 or functional variants or functional derivatives thereof,where SEQ ID NO: 10 is as follows:

LELVPRGSGDPIEGRGGGGGDPKSCDKPHTCPLCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKATPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNH YTQKSLSLSPGK

Variations and modifications to a recombinant human MIS protein andvectors can be used to increase or decrease recombinant human MISprotein expression, and to provide means for targeting. For example, arecombinant human MIS protein can be linked with a molecular targetingmolecule for targeting cancer cells or ovarian cells, to make therecombinant human MIS protein specific for cancers or tissue specific tothe ovary, respectively.

In one embodiment, a recombinant human MIS protein is fused to a secondfusion partner, such as a carrier molecule to enhance itsbioavailability. Such carriers are known in the art and include poly(alkyl) glycol such as poly ethylene glycol (PEG). Fusion to serumalbumin can also increase the serum half-life of therapeuticpolypeptides.

In some embodiments, a recombinant human MIS protein can also be fusedto a second fusion partner, for example, to a polypeptide that targetsthe product to a desired location, or, for example, a tag thatfacilitates its purification, if so desired. In some embodiments, tagsand fusion partners can be designed to be cleavable, if so desired.Another modification specifically contemplated is attachment, e.g.,covalent attachment, to a polymer. In one aspect, polymers such aspolyethylene glycol (PEG) or methoxypolyethylene glycol (mPEG) canincrease the in vivo half-life of proteins to which they are conjugated.Methods of PEGylation of polypeptide agents are well known to thoseskilled in the art, as are considerations of, for example, how large aPEG polymer to use.

In some embodiments, a recombinant human MIS protein or functionalfragment thereof is modified to achieve adequate circulating half-lives,which impact dosing, drug administration and efficacy. Many approacheshave been undertaken with the aim to increase the half-life ofbiotherapeutics. Small proteins below 60 kD are cleared rapidly by thekidney and therefore do not reach their target. This means that highdoses are needed to reach efficacy. The modifications to a recombinanthuman MIS protein and fragments encompassed in the methods of thepresent invention to increase the half-life of proteins in circulationinclude: PEGylation; conjugation or genetic fusion with proteins, e.g.,transferrin (WO06096515A2), albumin, growth hormone (US2003104578AA);conjugation with cellulose (Levy and Shoseyov, 2002); conjugation orfusion with Fc fragments; glycosylation and mutagenesis approaches(Carter, 2006), which are incorporated herein by reference.

In the case of PEGylation, polyethylene glycol (PEG) is conjugated to arecombinant human MIS protein or fragment, which can be for example aplasma protein, antibody or antibody fragment. The first studiesregarding the effect of PEGylation of antibodies were performed in the1980s. The conjugation can be done either enzymatically or chemicallyand is well established in the art (Chapman, 2002; Veronese and Pasut,2005). With PEGylation the total size can be increased, which reducesthe chance of renal filtration. PEGylation further protects fromproteolytic degradation and slows the clearance from the blood. Further,it has been reported that PEGylation can reduce immunogenicity andincrease solubility. The improved pharmacokinetics by the addition ofPEG is due to several different mechanisms: increase in size of themolecule, protection from proteolysis, reduced antigenicity, and themasking of specific sequences from cellular receptors. In the case ofantibody fragments (Fab), a 20-fold increase in plasma half-life hasbeen achieved by PEGylation (Chapman, 2002).

To date there are several approved PEGylated drugs, e.g., PEG-interferonalpha2b (PEG-INTRON) marketed in 2000 and alpha2a (Pegasys) marketed in2002. A PEGylated antibody fragment against TNF alpha, called Cimzia orCertolizumab Pegol, was filed for FDA approval for the treatment ofCrohn's disease in 2007 and has been approved on Apr. 22, 2008. Alimitation of PEGylation is the difficulty in synthesizing longmonodisperse species, especially when PEG chains over 1000 kD areneeded. For many applications, polydisperse PEG with a chain length over10000 kD is used, resulting in a population of conjugates havingdifferent length PEG chains, which need extensive analytics to ensureequivalent batches between productions. The different length of the PEGchains may result in different biological activities and thereforedifferent pharmacokinetics. Another limitation of PEGylation is adecrease in affinity or activity as it has been observed withalpha-interferon Pegasys, which has only 7% of the antiviral activity ofthe native protein, but has improved pharmacokinetics due to theenhanced plasma half-life.

In some embodiments, a recombinant human MIS protein or fragment thereofis conjugated with a long lived protein, e.g. albumin, which is 67 kDand has plasma half-life of 19 days in human (Dennis et al., 2002).Albumin is the most abundant protein in plasma and is involved in plasmapH regulation, but also serves as a carrier of substances in plasma. Inthe case of CD4, increased plasma half-life has been achieved afterfusing it to human serum albumin (Yeh et al., 1992). Other examples forfusion proteins are insulin, human growth hormone, transferrin andcytokines (Ali et al., 1999; Duttaroy et al., 2005; Melder et al., 2005;Osborn et al., 2002a; Osborn et al., 2002b; Sung et al., 2003) and see(US2003104578A1, WO06096515A2, and WO07047504A2, herein incorporated inentirety by reference).

The effect of glycosylation on plasma half-life and protein activity hasalso been extensively studied. In the case of tissue plasminogenactivator (tPA) the addition of new glycosylation sites decreased theplasma clearance, and improved the potency (Keyt et al., 1994).Glycoengineering has been successfully applied for a number ofrecombinant proteins and immunoglobulins (Elliott et al., 2003; Raju andScallon, 2007; Sinclair and Elliott, 2005; Umana et al., 1999). Further,glycosylation influences the stability of immunoglobulins (Mimura etal., 2000; Raju and Scallon, 2006).

In some embodiments, a recombinant human MIS protein or fragmentsthereof can be fused to the Fc fragment of an IgG (Ashkenazi and Chamow,1997). The Fc fusion approach has been utilized, for example in the TrapTechnology developed by Regeneron (e.g. IL1 trap and VEGF trap). The useof albumin to extend the half-life of peptides has been described inUS2004001827A1. Positive effects of albumin have also been reported forFab fragments and scFv-HSA fusion protein (Smith et al., 2001). It hasbeen demonstrated that the prolonged serum half-life of albumin is dueto a recycling process mediated by the FcRn (Anderson et al., 2006;Chaudhury et al., 2003; Smith et al., 2001).

In some embodiments, a recombinant human MIS protein is conjugated to abiotinylated Fc protein, as disclosed in US application 2010/0209424,which is incorporated herein in its entirety by reference.

As used herein, the term “conjugate” or “conjugation” refers to theattachment of two or more entities to form one entity. For example, themethods of the present invention provide conjugation of a recombinanthuman MIS protein (i.e. SEQ ID NO: 2 or 3 or fragments or derivatives orvariants thereof) joined with another entity, for example a moiety suchas a first fusion partner that makes the recombinant human MIS proteinstable, such as Ig carrier particle, for example IgG1 Fc. The attachmentcan be by means of linkers, chemical modification, peptide linkers,chemical linkers, covalent or non-covalent bonds, or protein fusion orby any means known to one skilled in the art. The joining can bepermanent or reversible. In some embodiments, several linkers can beincluded in order to take advantage of desired properties of each linkerand each protein in the conjugate. Flexible linkers and linkers thatincrease the solubility of the conjugates are contemplated for use aloneor with other linkers as disclosed herein. Peptide linkers can be linkedby expressing DNA encoding the linker to one or more proteins in theconjugate. Linkers can be acid cleavable, photocleavable and heatsensitive linkers. Methods for conjugation are well known by personsskilled in the art and are encompassed for use in the present invention.

According to the present invention, a recombinant human MIS protein(i.e. SEQ ID NO: 2 or 3 or fragments, derivatives or variants thereof),can be linked to the first fusion partner via any suitable means, asknown in the art, see for example U.S. Pat. Nos. 4,625,014, 5,057,301and 5, 514,363, which are incorporated herein in their entirety byreference. For example, a recombinant human MIS protein can becovalently conjugated to the IgG1 Fc, either directly or through one ormore linkers. In one embodiment, a recombinant human MIS protein asdisclosed herein is conjugated directly to the first fusion partner(e.g. Fc), and in an alternative embodiment, a recombinant human MISprotein as disclosed herein can be conjugated to a first fusion partner(such as IgG1 Fc) via a linker, e.g. a transport enhancing linker.

A large variety of methods for conjugation of a recombinant human MISprotein as disclosed herein with a first fusion partner (e.g. Fc) areknown in the art. Such methods are e.g. described by Hermanson (1996,Bioconjugate Techniques, Academic Press), in U.S. Pat. Nos. 6,180,084and 6,264,914 which are incorporated herein in their entirety byreference and include e.g. methods used to link haptens to carriersproteins as routinely used in applied immunology (see Harlow and Lane,1988, “Antibodies: A laboratory manual”, Cold Spring Harbor LaboratoryPress, Cold Spring Harbor, N.Y.). It is recognized that, in some cases,a recombinant human MIS protein can lose efficacy or functionality uponconjugation depending, e.g., on the conjugation procedure or thechemical group utilized therein. However, given the large variety ofmethods for conjugation the skilled person is able to find a conjugationmethod that does not or least affects the efficacy or functionality ofthe entities, such as a recombinant human MIS protein to be conjugated.

Suitable methods for conjugation of a recombinant human MIS protein asdisclosed herein with a first fusion partner (e.g. Fc) include e.g.carbodimide conjugation (Bauminger and Wilchek, 1980, Meth. Enzymol. 70:151-159). Alternatively, a moiety can be coupled to a targeting agent asdescribed by Nagy et al., Proc. Natl. Acad. Sci. USA 93:7269-7273(1996), and Nagy et al., Proc. Natl. Acad. Sci. USA 95:1794-1799 (1998),each of which are incorporated herein by reference. Another method forconjugating one can use is, for example sodium periodate oxidationfollowed by reductive alkylation of appropriate reactants andglutaraldehyde crosslinking.

One can use a variety of different linkers to conjugate a recombinanthuman MIS protein as disclosed herein with a first fusion partner (e.g.Fc), for example but not limited to aminocaproic horse radish peroxidase(HRP) or a heterobiofunctional cross-linker, e.g. carbonyl reactive andsulfhydryl-reactive cross-linker. Heterobiofunctional cross linkingreagents usually contain two reactive groups that can be coupled to twodifferent function targets on proteins and other macromolecules in a twoor three-step process, which can limit the degree of polymerizationoften associated with using homobiofunctional cross-linkers. Suchmulti-step protocols can offer a great control of conjugate size and themolar ratio of components.

The term “linker” refers to any means to join two or more entities, forexample a recombinant human MIS protein as disclosed herein with a firstfusion partner (e.g. Fc). A linker can be a covalent linker or anon-covalent linker. Examples of covalent linkers include covalent bondsor a linker moiety covalently attached to one or more of the proteins tobe linked. The linker can also be a non-covalent bond, e.g. anorganometallic bond through a metal center such as platinum atom. Forcovalent linkages, various functionalities can be used, such as amidegroups, including carbonic acid derivatives, ethers, esters, includingorganic and inorganic esters, amino, urethane, urea and the like. Toprovide for linking, the effector molecule and/or the probe can bemodified by oxidation, hydroxylation, substitution, reduction etc. toprovide a site for coupling. It will be appreciated that modificationwhich do not significantly decrease the function of a recombinant humanMIS protein as disclosed herein or the first fusion partner (e.g. Fc)are preferred.

Targeting.

In some embodiments, a recombinant human MIS protein, or functionalfragment, or a homologue for use in the methods and compositions asdisclosed herein can be targeted to a cancer or ovarian cells via atargeting ligand. A targeting ligand is a molecule, e.g., smallmolecule, protein or fragment thereof that specifically binds with highaffinity to a target, e.g., a cell-surface marker on a pre-selectedcell, such as a surface protein such as a receptor that is present to agreater degree on the pre-selected cell target than on any other bodytissue. Accordingly, in some embodiments, a recombinant human MISprotein for use in the compositions and methods as disclosed herein canbe fused to a Fc and/or optionally also to a targeting molecule. In someembodiments, a nucleic acid encoding a targeting ligand can be fused toa nucleotide encoding a recombinant human MIS protein or fragment orhomologue or variant thereof. Another example of a targeting ligand is agroup of cadherin domains from a human cadherin. A targeting ligandcomponent attached to a recombinant human MIS protein can include anaturally occurring or recombinant or engineered ligand, or a fragmentthereof, capable of binding the pre-selected target cell.

Further examples of targeting ligands also include, but are not limitedto, antibodies and portions thereof that specifically bind apre-selected cell surface protein with high affinity. By “high affinity”is meant an equilibrium dissociation constant of at least molar, asdetermined by assay methods known in the art, for example, BiaCoreanalysis. In one embodiment, the targeting ligand may also comprise oneor more immunoglobulin binding domains isolated from antibodiesgenerated against a selected tissue-specific surface protein or targettissue-specific receptor. The term “immunoglobulin or antibody” as usedherein refers to a mammalian, including human, polypeptide comprising aframework region from an immunoglobulin gene or fragments thereof thatspecifically binds and recognizes an antigen, which, in the case of thepresent invention, is a tissue-specific surface protein, a targettissue-specific receptor, or portion thereof. If the intended targetingfusion polypeptide will be used as a mammalian therapeutic,immunoglobulin binding regions should be derived from the correspondingmammalian immunoglobulins. If the targeting fusion polypeptide isintended for non-therapeutic use, such as for diagnostics and ELISAs,the immunoglobulin binding regions may be derived from either human ornon-human mammals, such as mice. The human immunoglobulin genes or genefragments include the kappa, lambda, alpha, gamma, delta, epsilon, andmu constant regions, as well as the myriad immunoglobulin variableregion genes. Light chains are classified as either kappa or lambda.Heavy chains are classified as gamma, mu, alpha, delta, or epsilon,which in turn define the immunoglobulin classes, lgG, lgM, IgA, IgD, andIgE, respectively. Within each lgG class, there are different isotypes(e.g. lgG1, lgG2, etc.). Typically, the antigen-binding region of anantibody will be the most critical in determining specificity andaffinity of binding.

An exemplary immunoglobulin (antibody) structural unit of human lgG,comprises a tetramer. Each tetramer is composed of two identical pairsof polypeptide chains, each pair having one light chain (about 25 kD)and one heavy chain (about 50-70 kD). The N-terminus of each chaindefines a variable region of about 100-110 or more amino acids primarilyresponsible for antigen recognition. The terms “variable light chain”(VL) and variable heavy chain (VH) refer to these light and heavy chainsrespectively. Antibodies exist as intact immunoglobulins, or as a numberof well-characterized fragments produced by digestion with variouspeptidases. For example, pepsin digests an antibody below the disulfidelinkages in the hinge region to produce F(ab)′2, a dimer of Fab whichitself is a light chain joined to VH-CH by a disulfide bond. The F(ab)′2may be reduced under mild conditions to break the disulfide linkage inthe hinge region, thereby converting the F(ab)′2 dimer into an Fab′monomer. The Fab′ monomer is essentially Fab with part of the hingeregion. While various antibody fragments are defined in terms of thedigestion of an intact antibody, one of skill will appreciate that suchfragments may be synthesized de novo either chemically or by usingrecombinant DNA methodology. Thus, the terms immunoglobulin or antibody,as used herein, also includes antibody fragments either produced by themodification of whole antibodies, or those synthesized de novo usingrecombinant DNA methodologies (e.g., single chain Fv) (scFv)) or thoseidentified using phase display libraries (see, for example, McCaffertyet al. (1990) Nature 348:552-554). In addition, the fusion polypeptidesof the invention include the variable regions of the heavy (VH) or thelight (VL) chains of immunoglobulins, as well as tissue-specific surfaceprotein and target receptor-binding portions thereof. Methods forproducing such variable regions are described in Reiter, et al. (1999)J. Mo!. Biol. 290:685-698.

Methods for preparing antibodies are known to the art. See, for example,Kohler & Milstein (1975) Nature 256:495-497; Harlow & Lane (1988)Antibodies: a Laboratory Manual, Cold Spring Harbor Lab., Cold SpringHarbor, N.Y.). The genes encoding the heavy and light chains of anantibody of interest can be cloned from a cell, e.g., the genes encodinga monoclonal antibody can be cloned from a hybridoma and used to producea recombinant monoclonal antibody. Gene libraries encoding heavy andlight chains of monoclonal antibodies can also be made from hybridoma orplasma cells. Random combinations of the heavy and light chain geneproducts generate a large pool of antibodies with different antigenicspecificity. Techniques for the production of single chain antibodies orrecombinant antibodies (U.S. Pat. Nos. 4,946,778; 4,816,567) can beadapted to produce antibodies used in the fusion polypeptides andmethods of the instant invention. Also, transgenic mice, or otherorganisms such as other mammals, may be used to express human orhumanized antibodies. Alternatively phage display technology can be usedto identify antibodies, antibody fragments, such as variable domains,and heteromeric Fab fragments that specifically bind to selectedantigens.

Screening and selection of preferred immunoglobulins (e.g., antibodies)can be conducted by a variety of methods known to the art: Initialscreening for the presence of monoclonal antibodies specific to atissue-specific or target receptor may be conducted through the use ofELISA-based methods or phage display, for example. A secondary screen ispreferably conducted to identify and select a desired monoclonalantibody for use in construction of the tissue-specific fusionpolypeptides of the invention. Secondary screening may be conducted withany suitable method known to the art. One method, termed “BiosensorModification-Assisted Profiling” (“BiaMAP”) (US patent publication2004/101920), allows rapid identification of hybridoma clones producingmonoclonal antibodies with desired characteristics. More specifically,monoclonal antibodies are sorted into distinct epitope-related groupsbased on evaluation of antibody: antigen interactions.

Production of Recombinant Human MIS Proteins

Recombinant human MIS proteins as disclosed herein, and functionalfragments and derivatives thereof can be obtained by any suitablemethod. For example, polypeptides can be produced using conventionalrecombinant nucleic acid technology such as DNA or RNA, preferably DNA.Guidance and information concerning methods and materials for productionof polypeptides using recombinant DNA technology can be found innumerous treatises and reference manuals. See, e.g., Sambrook et al,1989, Molecular Cloning—A Laboratory Manual, 2nd Ed., Cold Spring HarborPress; Ausubel et al. (eds.), 1994, Current Protocols in MolecularBiology, John Wiley & Sons, Inc.; Innis et al. (eds.), 1990 PCRProtocols, Academic Press.

Alternatively, recombinant human MIS proteins or functional fragmentsthereof can be obtained directly by chemical synthesis, e.g., using acommercial peptide synthesizer according to vendor's instructions.Methods and materials for chemical synthesis of polypeptides are wellknown in the art. See, e.g., Merrifield, 1963, “Solid Phase Synthesis,”J. Am. Chem. Soc. 83:2149-2154.

In some embodiments, a recombinant human MIS protein, or functionalfragment or derivative or variant thereof can be expressed in the cellfollowing introduction of a DNA encoding the protein, e.g., a nucleicacid encoding recombinant human MIS proteins or homologues or functionalderivatives thereof, e.g., in a conventional expression vector asdisclosed herein or by a catheter or by cells transformed with thenucleic acid ex vivo and transplanted into the subject.

Assays to Determine the Activity of the Recombinant Human MIS Protein

In one embodiment, an Organ Culture Assay System can be used to assaythe bioactivity of a human recombinant MIS protein as disclosed herein.The assay system used was described by Donahoe et al, J. Surg. Res., 23,141-148, 1977 which is the Mullerian regression organ culture assay. Theurogenital ridge was dissected from the 14-day female rat embryo andtransferred to an organ culture dish (Falcon, 3010). Specimens wereplaced on stainless-steel grids coated with a thin layer of 2% agar andincubated for 72 hr at 37 .degree. C. in 5% CO.sub.2 and 95% air over 2ml of culture medium [CMRL 1066 containing 10% fetal calf serum, 1%penicillin (10,000 units/ml)] or a 1:1 mixture of culture medium and thesupernatant or gradient fraction to be tested. The incubated tissue wasthen coated with a mixture of 2% agar and albumin at 44 .degree. C.,fixed in buffered formaldehyde, dehydrated in ethanol, cleaned inxylene, and embedded in paraffin. Eight-micrometer serial sections werestained with hematoxylin and eosin for viewing by light microscopy.Sections from the cephalic end of the Mullerian duct were assigned acoded number and graded for regression (Donahoe et al, Biol. Reprod.,15, 329-334, 1976) on a scale of 0 to V. Five slides with six to eightsections per slide were read for each assay. A grade of activity waslisted as the nearest whole number to the mean. A test group for thefractionation procedures represents at least 10 assays. If the mean fellmidway between two numbers, then both numbers were listed. Grade 0refers to no regression. The Mullerian duct, which is lined withcolumnar epithelial cells whose nuclei have a basilar orientation, has awidely patent lumen. Grade I is minimal regression. The duct is slightlysmaller, and either the surrounding mesenchyme is condensed around theduct as seen in plastic sections or there is a clear area around theduct as seen in paraffin sections. Grade II refers to mild regression.The duct is smaller, and the mesenchymal condensation or the clear areaaround the duct is more pronounced. The nucleii of the shorterepithelial cells loose their basilar orientation. Grade III is moderateregression. The duct is very small and disorganized. The tip of theurogenital ridge develops poorly distal to the Wolffian duct. Grade IVis severe regression. The duct is replaced by a whorl of cells. Grade Vrefers to complete regression. No remnant of the duct can be detected.Positive tissue controls, using fetal testis, and negative tissuecontrols, where the Mullerian ducts were incubated alone or with musclewere included in each experiment. Mullerian ducts exposed to extractsfrom nontesticular tissue, to inactive testicular fractions, or tosaline served as biochemical controls. Aliquots of all fractions weredialyzed against distilled water and freeze-dried, and protein contentwas measured.

Delivery of Recombinant Human MIS Protein

Methods known in the art for the therapeutic delivery of a recombinanthuman MIS protein and/or nucleic acids encoding the same can be used fortreating a disease or disorder, such as cancer in a subject, e.g.,cellular transfection, gene therapy, direct administration with adelivery vehicle or pharmaceutically acceptable carrier, indirectdelivery by providing recombinant cells comprising a nucleic acidencoding a targeting fusion polypeptide of the invention.

In some embodiments, the recombinant human MIS protein is cleaved invitro to form a bioactive halo-dimer of MIS, comprising two identicalmonomers, each consisting of the N-terminal domain and the C-terminaldomain, and then administered to a subject.

Various delivery systems are known and can be used to administer arecombinant human MIS protein (before or after it has been cleaved intoits bioactive form) to a subject, e.g., encapsulation in liposomes,microparticles, microcapsules, recombinant cells capable of expressingthe compound, receptor-mediated endocytosis (see, e.g., Wu and Wu, 1987,J. Biol. Chem. 262:4429-4432), construction of a nucleic acid as part ofa retroviral or other vector, etc. Methods of introduction can beenteral or parenteral and include but are not limited to intradermal,intramuscular, intraperitoneal, intravenous, subcutaneous, pulmonary,intranasal, intraocular, epidural, and oral routes. A recombinant humanMIS protein can be administered by any convenient route, for example byinfusion or bolus injection, by absorption through epithelial ormucocutaneous linings (e.g., oral mucosa, rectal and intestinal mucosa,etc.) and may be administered together with other biologically activeagents. Administration can be systemic or local. In addition, it may bedesirable to introduce the pharmaceutical compositions comprising arecombinant human MIS protein, before or after cleavage into itsbioactive form, into the central nervous system by any suitable route,including intraventricular and intrathecal injection; intraventricularinjection may be facilitated by an intraventricular catheter, forexample, attached to a reservoir, such as an Ommaya reservoir. Pulmonaryadministration can also be employed, e.g., by use of an inhaler ornebulizer, and formulation with an aerosolizing agent.

Methods to Treat Proliferative Diseases and Cancer

One aspect of the present invention provides methods for treatingcancers, e.g., cancers which express MISRII in a subject. Accordingly,one aspect of the present invention relates generally to a method oftreating a proliferative disease or disorder in a subject, where theproliferative disease or disorder is associated with cells that expressa MIS receptor, for example cells expressing MISRII. In someembodiments, the proliferative disease or disorder is cancer, where thecancer or cancer cells express at least one MIS receptor, for examplecancer or cancer cells expressing MISRII. The method of the presentinvention comprises the administration of an effective amount of arecombinant human MIS protein as disclosed herein or a functionalfragment or derivative thereof to a subject in with a proliferativedisorder, where the cells associated with the proliferative disorderexpress at least one MIS receptors, for example the cells expressMISRII. For example, an effective amount of a recombinant human MISprotein as disclosed herein or a functional fragment is administered toa subject with a cancer expressing at least one MIS receptors, forexample expressing MISRII. Thus, by using the methods of the presentinvention, one can intervene in the proliferative disease, for examplecancer, ameliorate the symptoms, and in some cases cure the disease. Insome embodiments, the recombinant human MIS protein that can be used fortreating proliferative diseases and cancer comprises the amino acidresidues 25-559 of SEQ ID NO: 2 or a functional fragment thereof.

Examples of such diseases where proliferation of cells expressing atleast one MIS receptors, for example expressing MISRII is the cause ofdisease are cancers, for example cervical cancer and ovarian cancer. Insome embodiments, the cancer expressing at least one MIS receptor, forexample MISRII is a cancer cell. In some embodiments, such a cancer cellexpressing at least one MIS receptors, for example expressing MISRII is,for example but not limited to, an ovarian cancer cell, vulvar epidermalcarcinoma cell, cervical carcinoma cell, endometrial edenocarinaomacell, ovarian adenocarcinoma.

In alternative embodiments, the cancer expressing at least one MISreceptor, for example cancers expressing MISRII are for example but notlimited to; breast cancer, lung cancer, head and neck cancer, bladdercancer, stomach cancer, cancer of the nervous system, bone cancer, bonemarrow cancer, brain cancer, colon cancer, esophageal cancer,endometrial cancer, gastrointestinal cancer, genital-urinary cancer,stomach cancer, lymphomas, melanoma, glioma, bladder cancer, pancreaticcancer, gum cancer, kidney cancer, retinal cancer, liver cancer,nasopharynx cancer, ovarian cancer, oral cancers, bladder cancer,hematological neoplasms, follicular lymphoma, cervical cancer, multiplemyeloma, osteosarcomas, thyroid cancer, prostate cancer, colon cancer,prostate cancer, skin cancer, stomach cancer, testis cancer, tonguecancer, or uterine cancer.

In alternative embodiments, the present invention relates to the use ofa recombinant human MIS protein as disclosed herein or a functionalfragment or derivative or variant thereof for the treatment of anydisorder where administration of the MIS protein or a nucleic acidencoding MIS protein or activation MISRII is whole or part of thetherapeutic regime.

In some embodiments, the cancer is a MIS-responsive cancer, for examplebut not limited ovarian cancer and cervical cancer. In some embodiments,the cancer expresses MISRII, for example but not limited ovarian cancerand cervical cancer. In some embodiments, the disorder is a disorderassociated with excess androgen states, for example as disclosed in U.S.Pat. No. 6,673,352, which is incorporated in its entirety herein byreference. In some embodiments, the methods of the present invention areused in the treatment of prostatic cancer, polycysic ovarian disease,benign prostatic hypertrophy and precocious puberty.

In a related embodiment, a tissue to be treated is a tumor tissueexpressing at least one MIS receptor, for example expressing MISRII of asubject, for example the tumor tissue is, but not limited to a solidtumor, a metastases, a skin cancer, a breast cancer, an ovarian cancer,an cervical cancer, a hemangioma or angiofibroma and the like cancer.Typical solid tumor tissues treatable by the pharmaceutical compositionof the invention, includes for example, but not limited to tumors of thelung, pancreas, breast, colon, laryngeal, ovarian, and the like tissues.In some embodiment, the solid tumor tissue treatable by the presentmethods include thyroid, and the cancer type is medullary thyroidcancer.

In a related embodiment, the invention contemplates the practice of themethod of administering a composition comprising a recombinant human MISprotein as disclosed herein or a functional fragment in conjunction withother therapies such as conventional chemotherapy directed against solidtumors and for control of establishment of metastases. Theadministration of the compounds described herein is typically conductedprior to and/or at the same time and/or after chemotherapy, although itis also encompassed within the present invention to inhibit cellproliferation after a regimen of chemotherapy at times where the tumortissue will be responding to the toxic assault by inducing angiogenesisto recover by the provision of a blood supply and nutrients to the tumortissue. In addition, the pharmaceutical compositions of the inventionfor the treatment of proliferative disorders, for example cancer, can beadministrated prophylatically and/or before the development of a tumor,if the subject has been identified as to have a risk of developingcancer, for example to subjects that are positive for biomarkers ofcancer cells or tumors. Insofar as the present methods apply toinhibition of cell proliferation, the methods can also apply toinhibition of tumor tissue growth, to inhibition of tumor metastasesformation, and to regression of established tumors.

The presence of MISRII in the cells in fluids such as blood may beindicative of the presence of cancer. The presence of MISRII in fluidsor sites not near a tumor may be indicative of metastasis. In some suchembodiments, the compounds of the present invention are administered tothe subject, and in some embodiments the compounds of the presentinvention are administered to the subject in a pharmaceuticalcomposition comprising one or more additional therapies.

The inventive methods disclosed herein provide for the parenteral andoral administration of a recombinant human MIS protein as disclosedherein or a functional fragment or derivative thereof, in combinationwith other pharmaceutical compositions to subjects in need of suchtreatment. Parenteral administration includes, but is not limited to,intravenous (IV), intramuscular (IM), subcutaneous (SC), intraperitoneal(IP), intranasal, and inhalant routes. In the method of the presentinvention, a recombinant human MIS protein as disclosed herein or afunctional fragment or analogs thereof are preferably administeredorally. IV, IM, SC, and IP administration may be by bolus or infusion,and may also be by slow release implantable device, including, but notlimited to pumps, slow release formulations, and mechanical devices. Theformulation, route and method of administration, and dosage will dependon the disorder to be treated and the medical history of the subject. Ingeneral, a dose that is administered by subcutaneous injection will begreater than the therapeutically-equivalent dose given intravenously orintramuscularly. Preferably, the dose of compounds of the presentinvention will be administered at doses from about 0.1 mg to about 250mg. In some embodiments, the dose of compounds of the present inventionwill be from about 1 mg to about 60 mg.

The methods of the present invention for treating cancer expressing atleast one MIS receptor, for example expressing MISRII, are useful fortreatment of proliferation-related diseases or cancer, which isassociated with cells expressing at least one MIS receptor, for exampleMISRII, comprising contacting a tissue in which proliferation isoccurring, or is at risk for occurring, with a composition comprising atherapeutically effective amount of a recombinant human MIS protein asdisclosed herein or a functional fragment or functional derivativesthereof.

In some embodiments, the subject treated by the methods of the presentinvention in its many embodiments is a human subject, although it is tobe understood that the principles of the invention indicate that theinvention is effective with respect to all mammals. In this context, amammal is understood to include any mammalian species in which treatmentof diseases associated with cancer or a proliferative-related disorderis desirable, particularly agricultural and domestic mammalian species,as well as transgenic animals.

Methods to Treat Neurodegenerative Diseases and Disorders

According to one aspect of the present invention there is provided amethod of treating a condition or disease characterized by neuronal celldeath or impairment in a patient in need thereof, said method comprisingadministering to said patient an effective amount of at least one therecombinant human MIS protein (e.g., the polypeptide and/or the nucleicacid encoding a recombinant human MIS protein) as disclosed herein, or afunctional fragment or derivative or variant thereof.

In some embodiments, the recombinant human MIS protein (e.g., thepolypeptide and/or the nucleic acid encoding a recombinant human MISprotein) as disclosed herein, or a functional fragment or derivative orvariant thereof, can be used to treat a neurodegenerative disease ordisorder, such as a motor neuron degenerative disease such asamyotrophic lateral sclerosis (ALS), spinal muscular atrophy (SMA),Primary lateral sclerosis (PLS) and other motor neuron degenerativediseases. In some embodiments, the recombinant human MIS protein thatcan be used for treating neurodegenerative diseases comprises the aminoacid residues 25-559 of SEQ ID NO: 2 or a functional fragment thereof.

Other aspects of the technology as disclosed herein relates to a methodto treat a neurodegenerative disease, such as a motor neurondegenerative disease such as amyotrophic lateral sclerosis (ALS), spinalmuscular atrophy (SMA), Primary lateral sclerosis (PLS) in a subject,the method comprising administering an effective amount of a recombinantMIS protein, wherein the recombinant MIS protein comprises amodification of amino acid 450 of SEQ ID NO: 1 from Q to R, where therecombinant MIS optionally comprises a tag, and wherein the recombinantMIS protein results in at least one of the following; increases themotor neuron survival, prevents or decreases the rate of motor neurondegeneration, prevents or reduces the decrease in muscle strength,promotes muscle strength, decreases or prevents the activation ofastrocytes and/or microglia in the spinal cord in the subject.

In one embodiment, the neurodegenerative disease or disorder ischaracterized by neuronal cell death, and in some embodiments, by motorneuron death or a decrease in the numbers of motor neurons in thesubject. In some embodiments, the subject has a decrease in the numbersof upper motor neurons (UMNs) or lower motor neurons (LMNs) or both UMNsand LMNs. Typically, MNDs are progressive, degenerative disorders thataffect nerves in the upper or lower parts of the body. Generally, motorneuron diseases (MNDs) strike in middle age. Symptoms may includedifficulty swallowing, limb weakness, slurred speech, impaired gait,facial weakness and muscle cramps. Respiration may be affected in thelater stages of these diseases. The cause(s) of most MNDs are not known,but environmental, toxic, viral or genetic factors are all suspects.Motor neurons, including upper motor neurons and lower motor neurons,affect voluntary muscles, stimulating them to contract. Upper motorneurons originate in the cerebral cortex and send fibers through thebrainstem and the spinal cord, and are involved in controlling lowermotor neurons. Lower motor neurons are located in the brainstem and thespinal cord and send fibers out to muscles. Lower motor neuron diseasesare diseases involving lower motor neuron degeneration. When a lowermotor neuron degenerates, the muscle fibers it normally activates becomedisconnected and do not contract, causing muscle weakness and diminishedreflexes. Loss of either type of neurons results in weakness, muscleatrophy (wasting) and painless weakness are the clinical hallmarks ofMND.

In some embodiments, the recombinant human MIS protein (e.g., thepolypeptide and/or the nucleic acid encoding a recombinant human MISprotein) as disclosed herein, or a functional fragment or derivative orvariant thereof is useful for the treatment of motor neuron diseases(MNDs) or disorders including but not limited to: amyotrophic lateralsclerosis (ALS), also called Lou Gehrig's disease or classical motorneuron disease; progressive bulbar palsy, also called progressive bulbaratrophy; pseudobulbar palsy; primary lateral sclerosis (PLS);progressive muscular atrophy; spinal muscular atrophy (SMA, includingSMA type I, also called Werdnig-Hoffmann disease, SMA type II, and SMAtype III, also called Kugelberg-Welander disease); Fazio-Londe disease;Kennedy disease, also known as progressive spinobulbar muscular atrophy;congenital SMA with arthrogryposis or post-polio syndrome (PPS).

Although the inventors have demonstrated that MIS has been reported toact as a motor neuron survival factor in vitro (Wang et al., PNAS, 2005;102 (45); 16421-16425). This was also disclosed in US applicationUS2009/304675. However, Wang et al., and the '675 application did notdemonstrate or suggest or provide motivation for using a modifiedversion of the MIS protein as disclosed herein. Furthermore, asdisclosed herein, the recombinant MIS protein as disclosed herein issuprizingly more stable and has improved cleavage, increased yield,increased bioactivity and increased potency as compared to wild-typehuman MIS protein. There is no suggestion in Wang et al., and the '675application to make such modifications to human MIS to treatneurogenerative disease or disorders, such as, for example, motor neurondiseases including ALS.

Amyotrophic lateral sclerosis (ALS) is a progressive, fatal degenerativedisorder mainly, but not exclusively, affecting motor neurons. ALS ischaracterized by a loss of pyramidal cells in the cerebral motor cortex(i.e., giant Betz cells), neurons located in the anterior horn of thespinal cord and the cortical neurons that provide their afferent input,anterior spinal motor neurons and brain stem motor neurons, anddegeneration thereof into pyramidal cells. Sensory function generally isspared, as is cognitive function and oculomotor activity. ALS shows,from a clinical aspect, both upper motor neurons and lower motor neuronssigns, including progressive muscle weakness, atrophy and spasticity,and shows rapid clinical deterioration after onset of the disease, thusleading to death from respiratory failure, usually within three to fiveyears from the onset of symptoms.

Most patients suffer from the sporadic form of this disease, whileapproximately 10% (about 10%-15%) have familial (an inherited form) ALS.Mutations in several genes are known to cause this hereditary form.Genetic epidemiology of ALS has revealed at least six chromosomelocations accountable for the inheritance of disease (ALS1 to ALS6).Among these, three genes have been identified. The first was identifiedin 1993 as the cytosolic Cu/Zn superoxide dismutase (SOD-1) gene thataccounts for 20% of the autosomal dominant form of ALS (Rosen et al.,Nature, 1993 Mar. 4; 362(6415):59-62). The discovery of this primarygenetic cause of ALS has provided a basis for generating mouse modelsfor this disease. These models are useful for testing therapies thatmight aid in the treatment of ALS. The second gene discovered associatedwith ALS was named as Alsin (ALS2), a potential guanine-nucleotideexchange factor (GEF) responsible for the juvenile recessive form ofALS. The third gene associated with inherited ALS is ALS4 that encodesfor a DNA/RNA helicase domain containing protein called Senataxinidentified to be linked to the autosomal dominant form of juvenile ALS.Most recently, a mutation in the vesicle associated membraneprotein/synaptobrevin associated membrane protein B (VAPB) in a newlocus called ALS8, was reported to be associated with an atypical formof ALS. Mutations in ALS6 (fused in sarcoma) is also associated with 5%of ALS cases. Mutations in the gene encoding optineurin (OPTN) have alsobeen associated with familial ALS (FALS). Mutations in VCP(valosin-containing protein) account for 1 percent to 2 percent of casesof familial ALS and affects the cell's energy factories, calledmitochondria. ALS-causing mutations in VCP severely reduced the amountof ATP, an energy transfer molecule, made by the mitochondria. Thereduction in ATP left the cells more vulnerable to stresses andincreased their death. VCP mutations are also responsible for diseasesaffecting muscle, bone, and the brain's frontal cortex, likelyindicating the widespread effects of reduced energy production.Mutations in superoxide dismutase 1 (SOD1), TA-DNA binding protein(TARDBP) and fused in sarcoma/translated in liposarcoma (FUS also knownas TLS or ALS6), and hexanucleotide repeat expansions in C9orf72 are themost prevalent.

TABLE 1 shows a list of genes associated with ALS: Locus Gene Gene nameChromosome ALS 1 SOD1 Cu/Zn superoxide dismutase 1, soluble (amyotrophic21q22.11 lateral sclerosis 1 (adult)) ALS 2 ALS2 amyotrophic lateralsclerosis 2 (juvenile) homolog 2q33.2 (human). Alsin ALS 3 ALS3 Unknown18q21 ALS 4 SETX Senataxin 9q34.13 ALS 5 SPAST Spastin 2p24 ALS 6 FUSfusion (involved in t(12;16) in malignant liposarcoma) 16p11.2 ALS 7ALS7 Unknown 20p13 ALS 8 VAPB Vesicle-associated membraneprotein-associated protein B 20q13.33 ALS 9 ANG Angiogenin 14q11.1 ALS10 TARDBP TAR DNA binding protein 1p36.22 ALS 11 FIG4 FIG4 homolog, SAC1lipid phosphatase domain 6q21 containing (S. cerevisiae) ALS 12 OPTNoptineurin 10p13 ALS 13 ATXN2 ataxin 2 12q23-q24.1 ALS 14 VCPvalosin-containing protein 9p13 ALS 15 UBQLN2 ubiquilin 2 Xp11.21 ALS 16SIGMAR1 sigma non-opioid intracellular receptor 1 9p13 ALS 17 ALS17Unknown 3p11.2 ALS 18 PFN1 profilin 1 17p13.3 ALS- ALS- Unknown 9q21-q22FTD 1 FTD1 ALS- C9orf72 chromosome 9 open reading frame 72 9p21.2 FTD 2ALS- CHMP2B chromatin modifying protein 2B 3p12.1 FTD 3 ALS UNC13Aunc-13 homolog A (C. elegans) 19p13.12 ALS DAO D-amino-acid oxidase12q24 ALS DCTN1 Dynactin 2p13 ALS NEFH neurofilament, heavy polypeptide200 kDa, heavy chain 22q12.1-q13.1 ALS PRPH peripherin 12q12 ALS SQSTM1sequestosome 1 5q35 ALS TAF15 TAF15 RNA polymerase II, TATA box bindingprotein 17q11.1-q11.2 (TBP)-associated factor, 68 kDa ALS SPG11 spasticparaplegia 11 (autosomal recessive) 15q14 ALS ELP3 elongation protein 3homolog (S. cerevisiae) 8p21.1

Although survival of ALS patients is only three to five years onaverage, variability of disease duration is quite large, ranging from afew months only to several decades. Even survival of patients with thesame mutation in the same gene in the same family is very variable.Similarly, age of onset can range from second to ninth decade of life.Genetic factors are expected to explain this variability by modifyingthe phenotype both in sporadic and familial ALS. Small animal modelssuch as flies, worms and zebrafish and mouse and animal rat models arevery useful for compound screening, and thus can be used for assessingthe efficacy of a recombinant human MIS protein (e.g., the polypeptideand/or the nucleic acid encoding a recombinant human MIS protein) asdisclosed herein, or a functional fragment or derivative or variantthereof for the effective treatment of a motor neuron disease. Such invivo animal models are well known to one of ordinary skill in the art,which include the SOD1^(G93A) mutant mouse as disclosed herein in theExamples, and other transgenic mice expressing human SOD1 with differentmutations, and a zebrafish models for ALS as disclosed in Internationalpatent application WO2012156351, where overexpressing mutant SOD1 orTDP-43 in zebrafish embryos induces a motor axonopathy, characterized byshorter and aberrantly branched motor axons. Transgenic animals (e.g.mice) that serve as models for MNDs, include but are not limited to, theSOD1^(G93A) mutant mouse or Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89AhmbSmn1tm1Msd/J mouse (Jackson lab stock number 006570).

In some embodiments, the neurodegenerative disease or disorder ischaracterized by neuronal cell impairment, for example, but not limitedto, decreased axonal transport, decreased mitochondria or decreasedmitochondrial function, an increase in protein aggregation and the like.

Another aspect of invention provides a method of modulating neuronalcell function in a subject in need thereof, the method comprising thestep of administering to said patient an effective amount of at leastone recombinant human MIS protein (e.g., the polypeptide and/or thenucleic acid encoding a recombinant human MIS protein) as disclosedherein, or a functional fragment or derivative or variant thereof. Insome embodiments, the administering is administering a virus vectorexpressing the recombinant human MIS variant as disclosed herein. Insome embodiments, the virus vector is an adeno-associated virus (AAV).

According to another aspect there is provided a method of enhancingneuronal cell survival in a subject in need thereof, the methodcomprising the step of administering to said patient an effective amountof at least one recombinant human MIS protein (e.g., the polypeptideand/or the nucleic acid encoding a recombinant human MIS protein) asdisclosed herein, or a functional fragment or derivative or variantthereof. In some embodiments, the administering is administering a virusvector expressing the recombinant human MIS variant as disclosed herein.In some embodiments, the virus vector is an AAV.

In some embodiments, the at least one recombinant human MIS protein(e.g., the polypeptide and/or the nucleic acid encoding a recombinanthuman MIS protein) as disclosed herein, or a functional fragment orderivative or variant thereof may induce neuronal cell differentiationand prevent the death and/or degeneration of neuronal cells both invitro and in vivo.

In some embodiments, the at least one recombinant human MIS protein(e.g., the polypeptide and/or the nucleic acid encoding a recombinanthuman MIS protein) as disclosed herein, or a functional fragment orderivative or variant thereof can also modulate neuronal cell functionin neurons that are dysfunctional.

In some embodiments, the at least one recombinant human MIS protein(e.g., the polypeptide and/or the nucleic acid encoding a recombinanthuman MIS protein) as disclosed herein, or a functional fragment orderivative or variant thereof can decrease the level of activatedastrocytes and/or microglia in neuronal tissues, e.g., the spinal cordand/or brain of subjects.

In some embodiments, the present invention provides a method oftreatment, or prevention or diagnosis of conditions where neurons aredysfunctional and/or degenerating, including but not limited to,neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS),primary lateral sclerosis (PLS), spinal muscular atrophy (SMA),Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease(HD), Friedreich's ataxia, cerebellar ataxia, other brain disorders suchas bipolar disorder, epilepsy, schizophrenia, depression, mania, autism,ADHD, brain trauma injuries and stroke.

In one embodiment, the neurons are located in the ventral horn of thespinal cord, or in regions of the brain comprising the cerebellumincluding but not limited to purkinje cells, the midbrain including butnot limited to the substantia nigra, the forebrain including by notlimited to the cerebral cortex, including but not limited to the caudateand putamen, the cerebrum, the hippocampus, the hypothalamus and thethalamus.

In some embodiment, the at least one one recombinant human MIS protein(e.g., the polypeptide and/or the nucleic acid encoding a recombinanthuman MIS protein) as disclosed herein, or a functional fragment orderivative or variant thereof is administered in conjunction with atleast one additional active compound. In some embodiments, theadditional compound is selected from the list comprising neurotrophicfactors including but not limited to glial cell line-derivedneurotrophic factor (GDNF), brain derived neurotrophic factor (BDNF),ciliary derived neurotrophic factor (CNTF), glutamate, and gonadalhormones including but not limited to estrogen, progesterone, androgenand synthetic equivalents thereof. In some embodiments, the activecompound is a therapeutic agent used in the treatment of aneurodegenerative disease or disorder, e.g., the treatment of a motorneuron disease, and can be selected from the group consisting of:riluzole (RILUTEK™), baclofen or diazepam (to help control spasticity),Gabapentin (to help control pain) and Trihexyphenidyl or amitriptyline(to help patients swallow saliva), as well as nucleic acid inhibitors tomutant proteins which are associated with ALS (e.g., siRNA SOD1).

Another aspect of the present invention relates to the use of at leastat least one recombinant human MIS protein (e.g., the polypeptide and/orthe nucleic acid encoding a recombinant human MIS protein) as disclosedherein, or a functional fragment or derivative or variant thereof in themanufacture of a medicament for treating a condition or diseasecharacterized by neuronal cell death or impairment in a patient in needthereof.

Another aspect of the present invention relates to the use of at leastat least one recombinant human MIS protein (e.g., the polypeptide and/orthe nucleic acid encoding a recombinant human MIS protein) as disclosedherein, or a functional fragment or derivative or variant thereof in themanufacture of a medicament for modulating neuronal cell function in apatient in need thereof.

Another aspect of the present invention relates to the use of at leastat least one recombinant human MIS protein (e.g., the polypeptide and/orthe nucleic acid encoding a recombinant human MIS protein) as disclosedherein, or a functional fragment or derivative or variant thereof in themanufacture of a medicament for enhancing neuronal cell survival in apatient in need thereof. In some embodiments, the medicament is forenhancing motor neuron survival in a subject with a neurodegenerativedisease, e.g., a motor neuron disease.

In some embodiments the recombinant human MIS protein (e.g., thepolypeptide and/or the nucleic acid encoding a recombinant human MISprotein) as disclosed herein, or a functional fragment or derivative orvariant thereof or medicament comprising the same is formulated forsimultaneous, separate or sequential administration with at least oneadditional active compound selected from the list comprisingneurotrophic factors including but not limited to glial cellline-derived neurotrophic factor (GDNF), brain derived neurotrophicfactor (BDNF), ciliary derived neurotrophic factor (CNTF), glutamate,and gonadal hormones including but not limited to estrogen,progesterone, androgen and synthetic equivalents thereof.

Another aspect of the present invention relates to a pharmaceuticalcomposition comprising at least at least one recombinant human MISprotein (e.g., the polypeptide and/or the nucleic acid encoding arecombinant human MIS protein) as disclosed herein, or a functionalfragment or derivative or variant thereof that modulates neuronal cellfunction and/or promotes neuronal survival in a patient in need thereof,together with a pharmaceutically acceptable carrier or excipient.

Another aspect of the present invention relates to a pharmaceuticalcomposition comprising at least at least one recombinant human MISprotein (e.g., the polypeptide and/or the nucleic acid encoding arecombinant human MIS protein) as disclosed herein, or a functionalfragment or derivative or variant thereof that enhances neuronal cellsurvival in a patient in need thereof, together with a pharmaceuticallyacceptable carrier or excipient.

In one embodiment the pharmaceutical composition comprising at least onerecombinant human MIS protein (e.g., the polypeptide and/or the nucleicacid encoding a recombinant human MIS protein) as disclosed herein, or afunctional fragment or derivative or variant thereof is formulated forsimultaneous, separate or sequential administration with at least oneadditional active compound selected from the list comprisingneurotrophic factors including but not limited to glial cellline-derived neurotrophic factor (GDNF), brain derived neurotrophicfactor (BDNF), ciliary derived neurotrophic factor (CNTF), glutamate,and gonadal hormones including but not limited to estrogen,progesterone, androgen and synthetic equivalents thereof.

Suitable routes of administration for treatment of a neurodegenerativedisease or disorder include, for example, include oral, rectal,transmucosal, especially transnasal, intestinal or parenteral delivery,including intramuscular, subcutaneous and intramedullary injections aswell as intrathecal, direct intraventricular, intracardiac, e.g., intothe right or left ventricular cavity, into the common coronary artery,intravenous, intraperitoneal, intranasal, or intraocular injections.

Conventional approaches for drug delivery to the central nervous system(CNS) include: neurosurgical strategies (e.g., intracerebral injectionor intracerebroventricular infusion); molecular manipulation of theagent (e.g., production of a chimeric fusion protein that comprises atransport peptide that has an affinity for an endothelial cell surfacemolecule in combination with an agent that is itself incapable ofcrossing the blood-brain barrier (BBB)) in an attempt to exploit one ofthe endogenous transport pathways of the BBB; pharmacological strategiesdesigned to increase the lipid solubility of an agent (e.g., conjugationof water-soluble agents to lipid or cholesterol carriers); and thetransitory disruption of the integrity of the BBB by hyperosmoticdisruption (resulting from the infusion of a mannitol solution into thecarotid artery or the use of a biologically active agent such as anangiotensin peptide). However, each of these strategies has limitations,such as the inherent risks associated with an invasive surgicalprocedure, a size limitation imposed by a limitation inherent in theendogenous transport systems, potentially undesirable biological sideeffects associated with the systemic administration of a chimericmolecule comprised of a carrier motif that could be active outside ofthe CNS, and the possible risk of brain damage within regions of thebrain where the BBB is disrupted, which renders it a suboptimal deliverymethod. In some embodiments, at least one recombinant human MIS protein(e.g., the polypeptide and/or the nucleic acid encoding a recombinanthuman MIS protein) as disclosed herein, or a functional fragment orderivative or variant thereof is administered systemically, e.g., byintravenous administration in the form of a vector, e.g., a viralvector, such as, but not limited to AAV.

Alternately, one may administer the pharmaceutical composition in alocal rather than systemic manner, for example, via injection of thepharmaceutical composition directly into a tissue region of a patient.

Uses

In another embodiment, the present invention provides a method fortreating a variety of conditions by administering an effective amount ofa recombinant human MIS protein or functional derivatives thereof of theinvention to a subject in need thereof. Conditions that may be treatedby the compounds of this invention, or a pharmaceutical compositioncontaining the same, include any condition which is treated or reducesthe symptoms by administration of MIS or activation of MIS signaling oractivation of MISRII, and thereby benefit from administration of arecombinant human MIS protein or functional derivatives thereof.Representative conditions in this regard include, for example, but notlimited to, cancers that express MIS receptors, for example cancer thatexpress MISRII, for example, but not limited to ovarian, cervical andendometrial cancer. Other conditions which can be treated with MIS oractivation of MIS signalling reduces the symptoms are, for example,rheumatoid arthritis, proliferative diseases such as cancer, treatmentof prostatic cancer, polycysic ovarian disease, benign prostatichypertrophy and precocious puberty and other hyperandrogen disorderssuch as testotoxicosis.

Accordingly, the present invention relates to the use of a recombinanthuman MIS protein or functional derivatives thereof for the treatment ofany disorder where administration of the MIS protein or a nucleic acidencoding MIS protein or a functional derivative of MIS or activationMISRII is whole, or part, of the therapeutic regime.

In some embodiments, the methods of the present invention are directedto use of a recombinant human MIS protein or functional derivativesthereof with other therapeutic agents, for example chemotherapy agents,wherein the chemotherapy agents, for example paclitaxel or MIS can beused at a lower dose that results in decreased side effects.

Uses of a Recombinant Human MIS Protein or Functional Derivatives orAnalogues Thereof for the Treatment of Excess Androgen States

In another embodiment, a recombinant human MIS protein or functionalderivatives or analogues thereof, can be used for the treatment of adisorder associated with excess androgen production in a subject. Theinventors have previously demonstrated that the administration of MISprotein and/or MIS nucleic acid decreases levels of androgen in asubject, and decreases serum levels of androgen in a subject, asdisclosed in U.S. Pat. No. 6,673,352 and U.S. patent application Ser.No. 10/683,346, which are incorporated herein in their entirety byreference. Transgenic mice that overexpress MIS have also shown to havedecreased serum testosterone concentrations, and administration of MISresults in decreased serum testosterone levels (Sriraman et al., JAndrol. 2001, 22(5):750-8 and Trbovich et al., PNAS, 2001 Mar. 13;98(6):3393-7). MIS has also been demonstrated to suppress bothandrogen-stimulated growth and androgen-independent survival of cells,and MIS regulates prostate growth by suppressing testicular testosteronesynthesis also direct regulates androgen-induced gene expression andgrowth in the prostate at the cellular level (Trann et al, MolEndocrinol. 2006, 20(10):2382-91).

Androgen stimulates or controls the development and maintenance ofmasculine characteristics in vertebrates by binding to androgenreceptors. Androgens are also known as androgenic hormones or testoids,and are also the precursor of all estrogens, the female sex hormones.The primary and most well-known androgen is testosterone.

Without wishing to be bound by theory, excessive androgen production bythe adrenal glands and/or the ovary, results in androgen excess and canresult from increased local tissue sensitivity to circulating androgens.Androgen excess affects different tissues and organ systems, causingclinical conditions ranging from acne to hirsutism to frankvirilization.

Hyperandrogenism, which refers to the excess production and secretion ofandrogens and precursors, is a common and sometimes seriousendrocrinopathy for women of reproductive age. The excess androgens andprecursors originate from the adrenal glands and ovaries in variousproportions and manifest in varying effects depending on the amount ofexcess androgen. Clinical manifestations range from hirsutism (excessivehair growth of male pattern, sometimes accompanied by acne) tovirilization (clitorimegaly, temporal balding, deepening of voice, orenhanced musculature).

Hyperandrogenism occurs as part of a wide spectrum of diseasemanifestations, including polycystic ovary syndrome (PCOS) which is avariable combination of hirsutism, infertility, obesity, insulinresistance and polycystic ovaries, the HAIR-AN syndrome(hyperandrogenism, insulin resistance and acanthosis nigricans), ovarianhyperthecosis (HAIR-AN with hyperplasia of luteinized theca cells inovarian stroma), and other manifestations of high intraovarian androgenconcentrations (e. g., follicular maturation arrest, atresia,anovulation, dysmenorrhea, dysfunctional uterine bleeding, infertility),androgenproducing tumors (virilizing ovarian or adrenal tumors).

Hirsutism is excessive recognizable hair growth characterized by anincrease in the number and length of terminal hairs inandrogen-sensitive areas. Racial, familial, genetic, and ethnicdifferences all affect the occurrence of hirsutism. Hirsutism isdifficult to quantitate. The entire body needs to be inspected and thefindings must be documented carefully. Particular attention should bedirected to the chin, lip, sideburns, breasts, and sternum, the midlinebetween the umbilicus and the pubis and the thigh.

Ferriman and Gallwey published a rating scale for grading hirsutism andis commonly known by persons of ordinary skill in the art. This scaleallows the physician to measure a response to therapy objectively. Thissystem is the most widely used and evaluates body areas forabsent-to-severe hirsutism with scores of 0-4, respectively. Scores of 8and higher are consistent with a diagnosis of hirsutism. This scale doesnot measure the thickness of the hair, which is another way ofobjectively assessing excess hair. Scoring systems are a useful aid inquantifying hirsutism and in evaluating treatment response. Even withscores greater than 8, the patient provides the definition. From aclinical standpoint, the patient can determine if he or she notices adifference. Photographs are helpful for documentation and for followingthe progress of therapy.

Virilization is relatively uncommon; it occurs with extremehyperandrogenism. Virilization is characterized by temporal balding,breast atrophy, androgenic muscle development, clitoral hypertrophy,amenorrhea, deepening of the voice, and extreme hirsutism.

Current medical therapies for women are directed against the adrenals,the ovaries or the androgen receptor. Glucocorticoid therapy is directedagainst the adrenal glands but is limited, in some cases, by unwantedsuppression of cortisol synthesis. GnRH therapy is directed against theovaries, but is expensive, and its long-term effects are unknown.Further, therapy using oral contraceptives may be unsuitable becausemost contain progestins with androgenic activity.

Because the abnormal production of androgens is implicated in thepathways of many diseases and/or disorders for which there are noacceptable treatments, a need exists to find small molecules to inhibitthe production of gonadotropins and/or androgens in mammals for theirtreatment and/or prophylaxis.

Accordingly, in one embodiment, a recombinant human MIS protein orfunctional derivatives or analogues thereof, can be used for thetreatment of a disorder associated with excess androgen production in asubject. In some embodiments, the recombinant human MIS protein that canbe used comprises the amino acid residues 25-559 of SEQ ID NO: 2 or afunctional fragment thereof.

The term “androgen” is used herein to mean steroids that encourage thedevelopment of male sex characteristics and include the steroidderivatives of androstane including, testosterone, androstenedione, andanalogs.

As used herein, a disease state or disorder characterized by “androgenicdependency” is a disease state which is exacerbated by, or caused by,insufficient, excessive, inappropriate or unregulated androgenproduction. Examples of such diseases in men include, but are notlimited to, BPH, metastatic prostatic carcinoma, testicular cancer,androgen dependent acne, male pattern baldness and precocious puberty inboys. Examples of such diseases in women include, but are not limitedto, hyperandrogenism, hirsutism, virilization, POCS, HAIR-AN syndrome,ovarian hyperthecosis, follicular maturation arrest, atresia,anovulation, dysmenorrhea, dysfunctional uterine bleeding, infertility,androgen-producing tumors.

As used herein, “androgen inhibiting” refers to an effective amount ofan the pyrazoloanthrone or functional derivatives or analogues thereofas defined herein, such as SP600125, which will cause a decrease in thein vivo levels of the androgen to normal or sub-normal levels, whenadministered to a subject for the prophylaxis or treatment of a diseasestate which is exacerbated by, or caused by, excessive or unregulatedandrogen production.

In some embodiments, a recombinant human MIS protein or functionalderivatives or analogues thereof as disclosed herein, can be used totreat prostate cancer. The impact of androgens on prostate carcinoma isknown, as is the treatment of prostate cancer by androgen deprivation,including androgen blockade and inhibition of androgen synthesis(Huggins et al., Archs. Surg., Vol. 43, pp. 209-223 (1941)). J. SteroidBiochem. Molec. Biol., Vol. 37, pp. 349-362 (1990)). In addition,steroid hormones are widely used as contraceptives. Anti-spermatogenicagents are male contraceptives that inhibit spermatogenesis, the processleading to mature spermatazoa. Drugs that interfere in this processinclude androgens and anti-androgens. Since the anti-androgenic effectsof a recombinant human MIS protein or functional derivatives oranalogues thereof as disclosed herein are reversible, the recombinanthuman MIS protein can also be used as a male contraceptive agent.Korolkovas, A., Essentials Of Medicinal Chemistry, Second Edition, pp.1032 (1988).

In some embodiments, other agents can be used in combination with thepharmaceutical compositions comprising a recombinant human MIS proteinor functional derivatives or analogues thereof as disclosed herein forthe treatment of excess androgen in a subject. In some embodiments, theagents function to lower the serum-free androgen levels and blocking theperipheral androgen action. Examples of such agents include, but are notlimited to, suppression of ovarian androgens by administration ofestrogens and/or progestins (i.e., contraceptive pill) or GnRH agonistand add-back estrogen therapy; suppression of adrenal androgens byadministration of glucocorticoids (such as dexamethasone, prednisolone),antiandrogens (such as spironolactone, flutamide, cyproterone acetate),5α-reductase inhibitor (such as finasteride), bromocriptine, andinsulin-sensitizing drugs (such as metformin, thiazolidinediones).

Subjects amenable to treatment with a recombinant human MIS protein orfunctional derivatives or analogues thereof by the methods as disclosedherein are subjects that have been identified with a disease or disorderassociated with excess androgen levels, such as, for example disorderssuch as, but not limited to BPH, prostate carcinoma, benign prostichypertrophy, testicular cancer, androgen dependent acne, male patternbaldness, precocious puberty, hyperandrogenism, hirsutism, virilization,POCS, HIAR-AN syndrome, ovarian hyperthecosis, follicular maturationarrest, atresia, anovulation, dysmenorrheal, dysfunctional uterinebleeding, infertility and androgen-producing tumors.

In some embodiments, subjects amenable to treatment with a recombinanthuman MIS protein or functional derivatives or analogues thereof by themethods as disclosed herein are subjects with congenical adrenalhyperplasma (CAH), which can be commonly identified by one of ordinaryskill in the art. CAH is most typically an autosomal recessive disorderwhere the enzyme 21-hydrolase is missing or functionally deficient.Alternatively subjects with CAH can have a loss and/or reduction in thefunction of 11α-hydroxylase enzyme and/or a 3α-hydroxy-steroiddehydrogenase enzyme. When these enzymes are missing or functioning atlow levels, the body cannot make adequate amounts of the adrenal steroidhormones cortisol and aldosterone. High levels of ACTH that stimulateadrenal hyperplasia and hypersecretion of androgen precursors forcortisol and aldosterone synthesis ensue. CAH can appear in utero ordevelop postnatally. Pseudohermaphroditism may be present at birth.

The 21-hydroxylase deficiency is the most common autosomal-recessivedisorder (more common than cystic fibrosis) and manifests itself withelevated levels of 17-hydroxyprogesterone. The 11α-hydroxylasedeficiency is characterized by elevated levels of 11-deoxy-cortisol(compound S) and results in elevated levels of deoxycorticosterone(DOC), a mineralocorticoid. Hypertension and hypokalemia can be aprominent feature of 11α-hydroxylase deficiency. Another form of CAH,3a-hydroxy-steroid dehydrogenase deficiency, results in elevated levelsof pregnenolone, 17-hydroxy-pregnenolone, and DHEA. This condition islethal if not detected because no corticosteroids are synthesized.

A partial defect in the above enzymes that manifests after pubertyresults in elevated levels of adrenal steroids via the same mechanism.The elevations are not as marked as they are with the congenitalcondition and this condition is referred to as nonclassical(maturity-onset or late-onset) CAH. Accordingly, in some embodiments,subjects amenable to treatment with a recombinant human MIS protein orfunctional derivatives or analogues thereof by the methods as disclosedherein are subjects with nonclassical (maturity-onset or late onset)CAH.

In some embodiments, subjects amenable to treatment with a recombinanthuman MIS protein or functional derivatives or analogues thereof by themethods as disclosed herein are female subjects with testosterone levelsabout or exceeding 2.0 ng/mL (200 ng/dL, 8.92 nmol/L) or at least about2.5 times the upper limit of the reference range. In some embodiments,such subjects have Sertoli-Leydig cell tumors, hilus cell tumors, andlipoid cell (adrenal rest) tumors are the most common. Sertoli-Leydigcell tumors reach palpable size at the time of clinical diagnosis,whereas hilar cell and lipoid cell tumors are difficult to detect by anymeans because of their small size.

In some embodiments, subjects amenable to treatment with a recombinanthuman MIS protein or functional derivatives or analogues thereof by themethods as disclosed herein are subjects with tumors of the adrenalglands (adenomas, carcinomas), which secrete elevated levels ofandrogens. In such embodiments, such subjects amenable to treatment bythe methods as disclosed herein can be identified by having a DHEASlevel of about or exceeding 7 μg/mL (18 μmol/L).

Other subjects that are amenable to the methods of treatment of excessandrogen states as disclosed herein include, for example, classical andnonclassical (late-onset) CAH, cushing syndrome, where subjects withCushing syndrome secrete elevated androgens, Hyperandrogenic, insulinresistance, and acanthosis nigricans (HAIR-AN) syndrome. In someembodiments, other subjects amenable to the methods of treatment ofexcess androgen states as disclosed herein include, for example,subjects with mild androgenic disorders, such as, but not limited to,Ovulatory PCOS (Ovulatory hyperandrogenic subjects with polycystic ovaryat ultrasonography), Idiopathic hyperandrogenism (an Ovulatoryhyperandrogenic subject but with normal ovaries at ultrasonography);Idiopathic hirsutism (subjects with an androgenic phenotype with normalandrogens).

Reference testosterone levels and DHEAS levels are commonly known bypersons of ordinary skill in the art, and are disclosed in Guay et al,International Journal of Impotence Research (2004) 16, 112-120, which isincorporated herein in its entirety by reference. Briefly, normalandrogen levels in women between the ages of 20 and 49 years rangebetween; DHEAS; about 195.6-140.4 ug/dl; serum testosterone about51.5-33.7 ng/dl and free testosterone 1.51-1.03 pg/ml. Accordingly,subjects amenable to the treatment of the pyrazoloanthrone or functionalderivatives or analogues thereof by the methods as disclosed herein haveat least about a 20%, or at least about a 30% or at least about a 40% orat least about a 50%, or at least about a 60% or at least about a 70%,or at least about a 80%, or at least about a 90%, or at least about a100% or greater increase in DHEAS or serum testosterone, or freetestosterone levels as compared to the highest range value of the normalvalue for DHEAS (195.6 μg/dl), serum testosterone (51.5 ng/dl), freetestostereone (1.51 pg/ml). In some embodiments, subjects amenable tothe treatment of the pyrazoloanthrone or functional derivatives oranalogues thereof by the methods as disclosed herein have at least abouta 2-fold, or at least about a 3-fold, or at least about a 4-fold, or atleast about a 5-fold, or at least about a 10-fold or greater increase inDHEAS or serum testosterone, or free testosterone levels as compared tothe highest range value of the normal value for DHEAS (195.6 μg/dl),serum testosterone (51.5 ng/dl), free testostereone (1.51 pg/ml).

DHEAS can be measured by one of ordinary skill in the art using a kitfrom by Diagnostic Products Corporation of Los Angeles, Calif., USA.Cross-reactivity has previously been determined as being 100% for DHEASand 0.121% with androstenedione, 15% with 9-hydroxyandrostenedione,0.046% with estrone 3 sulfate, 0.55% with androsterone sulfate, 0.5%with DHEA and negligible for all other steroids tested. FreeTestosterone can be measured by one of ordinary skill in the art usingwas measured using the Coat a Count Kits of Diagnostic ProductsCorporation, Los Angeles, Calif., USA. Cross-reactivity has previouslybeen determined to be 0.41% for dihydrotestosterone, 0.01% forandrostenedione, 0.10% for methyl testosterone ando0.01% for all othersteroids tested. Total serum testosterone levels can be measured by oneof ordinary skill in the art using with the Immunochem serumtestosterone kit of ICN Biomedicals Inc., Diagnostic Division of CostaMesa, Calif., USA.

The assays to determine serum pregnenolone and 17-hydroxypregnenolonecan be performed by one of ordinary skill in the art from the kit fromQuest Laboratory in Tarzana, Calif., USA. Free Androgen Index (FAI) canbe calculated using the following formula: (Total testosterone ng/dl x0.0347)/(SHBG nmol/1)×100=FAI.

Administration of Pharmaceutical Compositions

A recombinant human MIS protein or derivative or functional fragmentthereof can be administered by any route known in the art or describedherein, for example, oral, parenteral (e.g., intravenously orintramuscularly), intraperitoneal, rectal, cutaneous, nasal, vaginal,inhalant, skin (patch), or ocular. The recombinant human MIS protein orderivative or functional fragment protein may be administered in anydose or dosing regimen.

With respect to the therapeutic methods of the invention, it is notintended that the administration of a recombinant human MIS protein orpolynucleotide encoding such a recombinant human MIS protein orfunctional fragment thereof be limited to a particular mode ofadministration, dosage, or frequency of dosing; the present inventioncontemplates all modes of administration, including intramuscular,intravenous, intraperitoneal, intravesicular, intraarticular,intralesional, subcutaneous, or any other route sufficient to provide adose adequate to treat an autoimmune disease or immune-related disorderas disclosed herein. An effective amount, e.g., a therapeuticallyeffective dose of a recombinant human MIS protein may be administered tothe patient in a single dose or in multiple doses. When multiple dosesare administered, the doses may be separated from one another by, forexample, one hour, three hours, six hours, eight hours, one day, twodays, one week, two weeks, or one month. For example, a compositioncomprising a recombinant human MIS protein agent can be administeredfor, e.g., 2, 3, 4, 5, 6, 7, 8, 10, 15, 20, or more weeks. It is to beunderstood that, for any particular subject, specific dosage regimesshould be adjusted over time according to the individual need and theprofessional judgment of the person administering or supervising theadministration of the compositions. For example, the dosage of thetherapeutic can be increased if the lower dose does not providesufficient therapeutic activity.

While the attending physician ultimately will decide the appropriateamount and dosage regimen, an effective amounts of a recombinant humanMIS protein or derivative or functional fragment thereof can provided ata dose of 0.0001, 0.01, 0.01 0.1, 1, 5, 10, 25, 50, 100, 500, or 1,000mg/kg. Effective doses may be extrapolated from dose-response curvesderived from in vitro or animal model test bioassays or systems. In someembodiments, doses of a recombinant human MIS protein are about 1 pg/kgto 10 mg/kg (body weight of patient) although lower and higher doses canalso be administered.

In some embodiments, reference ranges for doses of recombinant human MISare estimated from reference groups in the United States, and aredisclosed in Antimullerian Hormone (AMH), Serum from Mayo MedicalLaboratories. Retrieved April 2012. In some embodiments, female subjectscan be administered the following doses of recombinant human MIS:females younger than 24 months: Less than 5 ng/mL; females 24 months to12 years: Less than 10 ng/mL; females 13-45 years: 1 to 10 ng/mL;females older than 45 years: Less than 1 ng/mL. In some embodiments,male subjects can be administered the following doses of recombinanthuman MIS; males younger than 24 months: 15 to 500 ng/m; males between24 months to 12 years: 7 to 240 ng/mL; males older than 12 years: 0.7 to20 ng/mL. It is noted that MIS measurements may be less accurate if theperson being measured is vitamin D deficient.

Additionally, as additivity, synergy, or competition has beendemonstrated with MIS and rapamycin, AzadC, doxorubicin, cisplatin, andpaclitaxel, recombinant human MIS as disclosed herein can beadministered in combination with selective targeted therapies, forexample to achieve greater activity against ovarian cancer than the useof recombinant human MIS or the chemotherapeutic agent used alone.

Dosages for a particular patient or subject can be determined by one ofordinary skill in the art using conventional considerations, (e.g. bymeans of an appropriate, conventional pharmacological protocol). Aphysician may, for example, prescribe a relatively low dose at first,subsequently increasing the dose until an appropriate response isobtained. The dose administered to a patient is sufficient to effect abeneficial therapeutic response in the patient over time, or, e.g., toreduce symptoms, or other appropriate activity, depending on theapplication. The dose is determined by the efficacy of the particularformulation, and the activity, stability or serum half-life of arecombinant human MIS protein or functional derivatives or functionalfragments thereof as disclosed herein, and the condition of the patient,the autoimmune disease to be treated, as well as the body weight orsurface area of the patient to be treated. The size of the dose is alsodetermined by the existence, nature, and extent of any adverseside-effects that accompany the administration of a particular vector,formulation, or the like in a particular subject. Therapeuticcompositions comprising a recombinant human MIS protein or functionalderivatives or functional fragments thereof are optionally tested in oneor more appropriate in vitro and/or in vivo animal models of disease,such a an Mullerian duct regression bioassay as disclosed herein in theExamples, and known to persons of ordinary skill in the art, to confirmefficacy, tissue metabolism, and to estimate dosages, according tomethods well known in the art. In particular, dosages can be initiallydetermined by activity, stability or other suitable measures oftreatment vs. non-treatment (e.g., comparison of treated vs. untreatedcells or animal models), in a relevant assay. Formulations areadministered at a rate determined by the LD50 of the relevantformulation, and/or observation of any side-effects of a recombinanthuman MIS protein or functional derivatives or functional fragmentsthereof at various concentrations, e.g., as applied to the mass andoverall health of the patient. Administration can be accomplished viasingle or divided doses.

In determining the effective amount of a recombinant human MIS proteinor functional derivatives or functional fragments thereof to beadministered in the treatment or prophylaxis of a disease, the physicianevaluates circulating plasma levels, formulation toxicities, andprogression of the disease. The selected dosage level will also dependupon a variety of factors including the activity of the particularcompound of the present invention employed, or the ester, salt or amidethereof, the route of administration, the time of administration, therate of excretion of the particular compound being employed, theduration of the treatment, other drugs, compounds and/or materials usedin combination with the particular compound employed, the age, sex,weight, condition, general health and prior medical history of thepatient being treated, and like factors well known in the medical arts.

In some embodiments, a recombinant human MIS protein as disclosed hereincan be administered at a dose in accordance with good medical practice,taking into account the clinical condition of the individual patient,the site and method of administration, scheduling of administration,patient age, sex, body weight and other factors known to medicalpractitioners.

Dosage regimens of a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereincan be adjusted to provide the optimum desired response (e.g. atherapeutic or prophylactic response). For example, a single bolus canbe administered, several divided doses may be administered over time orthe dose may be proportionally reduced or increased as indicated by theexigencies of the therapeutic situation. It is especially advantageousto formulate parenteral compositions in dosage unit form for ease ofadministration and uniformity of dosage.

Furthermore, actual dosage levels of a recombinant human MIS protein ina pharmaceutical composition can be varied so as to obtain an amount ofthe active ingredient which is effective to achieve the desiredtherapeutic response for a particular subject, composition, and mode ofadministration, without being toxic to the subject. A pharmaceuticalcomposition comprising a recombinant human MIS protein or functionalfragment or variant thereof as disclosed herein can be a“therapeutically effective amount” and/or a “prophylactically effectiveamount”. In general, a suitable daily dose of a composition comprising arecombinant human MIS protein or functional fragment or variant thereofas disclosed herein will be that amount of the a recombinant human MISprotein which is the lowest dose effective to produce a therapeuticeffect, such as a reduction of a symptom of a proliferative disorder orcancer as disclosed herein. Such an effective dose will generally dependupon the factors described above.

If desired, the effective daily dose of a composition comprising arecombinant human MIS protein or functional fragment or variant thereofcan be administered as two, three, four, five, six or more sub-dosesadministered separately at appropriate intervals throughout the day,optionally, in unit dosage forms.

The dosage level administered to a subject can be constant over adesired period of time, for example, at least 1 week, at least 2 weeks,at least 3 weeks, at least 1 month, at least 2 months, at least 3months, at least 6 months, at least 1 year, or at least 5 years.Alternatively, the dosage level administered to a subject can varydepending on the progression of the condition being treated.

It is to be noted that dosage values may vary with the type and severityof the cancer to be alleviated. It is to be further understood that forany particular subject, specific dosage regimens should be adjusted overtime according to the individual need and the professional judgment ofthe person administering or supervising the administration of thecompositions, and that dosage ranges set forth herein are exemplary onlyand are not intended to limit the scope or practice of the claimedcomposition.

The efficacy and toxicity of the compound can be determined by standardpharmaceutical procedures in cell cultures or experimental animals,e.g., ED50 (the dose is effective in 50% of the population) and LD50(the dose is lethal to 50% of the population). The dose ratio of toxicto therapeutic effects is the therapeutic index, and it can be expressedas the ratio, LD50/ED50. Pharmaceutical compositions which exhibit largetherapeutic indices are preferred. An appropriate experimental modelwhich can be used includes determining a the dose can be use of themullerian duct regression bioassay as disclosed herein in the examples,or a in vivo cancer model which is commonly known by ordinary skill inthe art. In vivo cancer models are discussed in Frese et al.,“Maximizing mouse cancer models” Nat Rev Cancer. 2007 September;7(9):645-58 and Santos et al., Genetically modified mouse models incancer studies. Clin Transl Oncol. 2008 December; 10(12):794-803, and“Cancer stem cells in mouse models of cancer”, 6th Annual MDI Stem CellSymposium, MDI Biological Lab, Salisbury Cove, Me., Aug. 10-11, 2007″which are incorporated herein in their entirety by reference.

For example, a therapeutically effective amount can be estimatedinitially either in cell culture assays or in animal models, usuallymice, rabbits, dogs, or pigs. The animal model is also used to achieve adesirable concentration range and route of administration. Suchinformation can then be used to determine useful doses and routes foradministration in other subjects. Generally, the therapeuticallyeffective amount is dependent of the desired therapeutic effect. Forexample, the therapeutically effective amount of a recombinant human MISprotein can be assessed in a mouse model of cancer, or using theMullerian Duct Regression bioassay as disclosed herein in the Examplesand FIG. 4.

A physician or veterinarian having ordinary skill in the art can readilydetermine and prescribe the effective amount of the pharmaceuticalcomposition required. For example, the physician or veterinarian couldstart doses of the compounds of the invention employed in thepharmaceutical composition at levels lower than that required in orderto achieve the desired therapeutic effect and gradually increase thedosage until the desired effect is achieved. It is also noted thathumans are treated generally longer than the mice or other experimentalanimals exemplified herein, which treatment has a length proportional tothe length of the disease process and drug effectiveness. The doses maybe single doses or multiple doses over a period of several days, butsingle doses are preferred.

In some embodiments, a recombinant human MIS protein (e.g., proteins ornucleic acids encoding a recombinant human MIS protein or fragmentsthereof) can be administered to humans and other animals for therapy byany suitable route of administration, including orally, nasally, as by,for example, a spray, rectally, intravaginally, parenterally,intracisternally and topically, as by powders, ointments or drops,including buccally and sublingually.

After formulation with an appropriate pharmaceutically acceptablecarrier in a desired dosage, a pharmaceutical composition comprising arecombinant human MIS protein or functional fragment or variant thereofas disclosed herein can be administered to a subject. A pharmaceutical acomposition comprising a recombinant human MIS protein or functionalfragment or variant thereof can be administered to a subject using anysuitable means. In general, suitable means of administration include,but are not limited to, topical, oral, parenteral (e.g., intravenous,subcutaneous or intramuscular), rectal, intracisternal, intravaginal,intraperitoneal, ocular, or nasal routes.

In a specific embodiment, it may be desirable to administer thepharmaceutical composition comprising a recombinant human MIS proteinlocally to the area in need of treatment; this may be achieved, forexample, and not by way of limitation, by local infusion during surgery,topical application, e.g., by injection, by means of a catheter, or bymeans of an implant, the implant being of a porous, non-porous, orgelatinous material, including membranes, such as sialastic membranes,fibers, or commercial skin substitutes. In some embodiments, arecombinant human MIS protein as disclosed herein can be applied to themuscle using topical creams, patches, intramuscular injections and thelike.

In some embodiments, a recombinant human MIS protein can be administeredto a subject orally (e.g., in capsules, suspensions or tablets) or byparenteral administration. Conventional methods for oral administrationinclude administering a recombinant human MIS protein in any one of thefollowing; tablets, suspensions, solutions, emulsions, capsules,powders, syrups and the like are usable. Known techniques that deliver arecombinant human MIS protein orally or intravenously and retain thebiological activity are preferred. Parenteral administration caninclude, for example, intramuscular, intravenous, intraarticular,intraarterial, intrathecal, subcutaneous, or intraperitonealadministration. A recombinant human MIS protein can also be administeredorally, transdermally, topically, by inhalation (e.g., intrabronchial,intranasal, oral inhalation or intranasal drops) or rectally.Administration can be local or systemic as indicated. Agents, e.g.,nucleic acid agents which encode a recombinant human MIS protein orfunctional fragment thereof can also be delivered using a vector, e.g.,a viral vector by methods which are well known to those skilled in theart.

When administering a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereinparenterally, it will generally be formulated in a unit dosageinjectable form (e.g., solution, suspension, emulsion). Thepharmaceutical formulations suitable for injection include sterileaqueous solutions or dispersions and sterile powders for reconstitutioninto sterile injectable solutions or dispersions. The carrier can be asolvent or dispersing medium containing, for example, water, ethanol,polyol (e.g., glycerol, propylene glycol, liquid polyethylene glycol),suitable mixtures thereof, and vegetable oils.

The term “Dosage unit” form as used herein refers to physically discreteunits suited as unitary dosages for the mammalian subjects to betreated; each unit containing a predetermined quantity of activecompound calculated to produce the desired therapeutic effect inassociation with the required pharmaceutical carrier. The specificationfor the dosage unit forms of the invention are dictated by and directlydependent on (a) the unique characteristics of the a recombinant humanMIS protein or functional fragment or variant thereof as disclosedherein and the particular therapeutic or prophylactic effect to beachieved, and (b) the limitations inherent in the art of compounding arecombinant human MIS protein an active agent for the treatment ofsensitivity in individuals.

The pharmaceutically acceptable compositions comprising a recombinanthuman MIS protein or functional fragment or variant thereof as disclosedherein can be suspended in aqueous vehicles and introduced throughconventional hypodermic needles or using infusion pumps.

Pharmaceutical Compositions

In some embodiments, a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereincan be formulated in any suitable means, e.g., as a sterile injectablesolution, e.g., which can be prepared by incorporating the recombinanthuman MIS protein in the required amount of the appropriate solvent withvarious of the other ingredients, as desired.

A pharmacological formulation of a composition comprising a recombinanthuman MIS protein or functional fragment or variant thereof as disclosedherein can be administered to the patient in an injectable formulationcontaining any compatible carrier, such as various vehicles, adjuvants,additives, and diluents; or the compounds utilized in the presentinvention can be administered parenterally to the patient in the form ofslow-release subcutaneous implants or targeted delivery systems such asmonoclonal antibodies, vectored delivery, iontophoretic, polymermatrices, liposomes, and microspheres. Examples of delivery systemsuseful in the present invention include those presented in U.S. Pat.Nos. 5,225,182; 5,169,383; 5,167,616; 4,959,217; 4,925,678; 4,487,603;4,486,194; 4,447,233; 4,447, 224; 4,439,196 and 4,475,196. Other suchimplants, delivery systems, and modules are well known to those skilledin the art.

Proper fluidity can be maintained, for example, by the use of a coatingsuch as lecithin, by the maintenance of the required particle size inthe case of dispersion and by the use of surfactants. Non-aqueousvehicles such a cottonseed oil, sesame oil, olive oil, soybean oil, cornoil, sunflower oil, or peanut oil and esters, such as isopropylmyristate, may also be used as solvent systems for compoundcompositions. Additionally, various additives which enhance thestability, sterility, and isotonicity of the compositions, includingantimicrobial preservatives, antioxidants, chelating agents, andbuffers, can be added. Prevention of the action of microorganisms can beensured by various antibacterial and antifungal agents, e.g., parabens,chlorobutanol, phenol and sorbic acid. In many cases, it will bedesirable to include isotonic agents, for example, sugars, sodiumchloride, and the like. Prolonged absorption of the injectablepharmaceutical form can be brought about by the use of agents delayingabsorption, for example, aluminum monostearate and gelatin. According tothe present invention, however, any vehicle, diluent, or additive usedwould have to be compatible with the compounds.

In another embodiment, a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereincan comprise lipid-based formulations. Any of the known lipid-based drugdelivery systems can be used in the practice of the invention. Forinstance, multivesicular liposomes, multilamellar liposomes andunilamellar liposomes can all be used so long as a sustained releaserate of the encapsulated active compound can be established. Methods ofmaking controlled release multivesicular liposome drug delivery systemsare described in PCT Application Publication Nos: WO 9703652, WO9513796, and WO 9423697, the contents of which are incorporated hereinby reference.

The composition of the synthetic membrane vesicle is usually acombination of phospholipids, usually in combination with steroids,especially cholesterol. Other phospholipids or other lipids may also beused. Examples of lipids useful in synthetic membrane vesicle productioninclude phosphatidylglycerols, phosphatidylcholines,phosphatidylserines, phosphatidylethanolamines, sphingolipids,cerebrosides, and gangliosides, with preferable embodiments includingegg phosphatidylcholine, dipalmitoylphosphatidylcholine,distearoylphosphatidyleholine, dioleoylphosphatidylcholine,dipalmitoylphosphatidylglycerol, and dioleoylphosphatidylglycerol.

In preparing lipid-based vesicles containing a recombinant human MISprotein or functional fragment or variant thereof, such variables as theefficiency of active compound encapsulation, labiality of the activecompound, homogeneity and size of the resulting population of vesicles,active compound-to-lipid ratio, permeability, instability of thepreparation, and pharmaceutical acceptability of the formulation shouldbe considered.

In another embodiment, a recombinant human MIS protein can be deliveredin a vesicle, in particular a liposome (see Langer (1990) Science249:1527-1533). In yet another embodiment, a recombinant human MISprotein can be delivered in a controlled release system. In oneembodiment, a pump may be used (see Langer (1990) supra). In anotherembodiment, polymeric materials can be used (see Howard et al. (1989) J.Neurosurg. 71:105). In another embodiment where the active agent of theinvention is a nucleic acid encoding a recombinant human MIS protein,the nucleic acid can be administered in vivo to promote expression ofits encoded protein, by constructing it as part of an appropriatenucleic acid expression vector and administering it so that it becomesintracellular, e.g., by use of a retroviral vector (see, for example,U.S. Pat. No. 4,980,286), or by direct injection, or by use ofmicroparticle bombardment (e.g., a gene gun; Biolistic, Dupont), orcoating with lipids or cell-surface receptors or transfecting agents, orby administering it in linkage to a homeobox-like peptide which is knownto enter the nucleus (see e.g., Joliot et al., 1991, Proc. Natl. Acad.Sci. USA 88:1864-1868), etc. Alternatively, a nucleic acid can beintroduced intracellularly and incorporated within host cell DNA forexpression, by homologous recombination.

Prior to introduction, a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereincan be sterilized, by any of the numerous available techniques of theart, such as with gamma radiation or electron beam sterilization.

In another embodiment of the invention, a composition comprising arecombinant human MIS protein or functional fragment or variant thereofas disclosed herein, can be administered and/or formulated inconjunction (e.g., in combination) with any other therapeutic agent. Forpurpose of administration, a recombinant human MIS protein or functionalfragment or variant thereof as disclosed herein is preferably formulatedas a pharmaceutical composition. Pharmaceutical compositions of thepresent invention comprise a compound of this invention and apharmaceutically acceptable carrier, wherein the compound is present inthe composition in an amount which is effective to treat the conditionof interest. Appropriate concentrations and dosages can be readilydetermined by one skilled in the art.

Pharmaceutically acceptable carriers are familiar to those skilled inthe art. For compositions formulated as liquid solutions, acceptablecarriers include saline and sterile water, and may optionally includeantioxidants, buffers, bacteriostats and other common additives. Thecompositions can also be formulated as pills, capsules, granules, ortablets which contain, in addition to a compound of this invention,diluents, dispersing and surface active agents, binders, and lubricants.One skilled in this art may further formulate the compounds of thisinvention in an appropriate manner, and in accordance with acceptedpractices, such as those disclosed in Remington's PharmaceuticalSciences, Gennaro, Ed., Mack Publishing Co., Easton, Pa. 1990.

The compositions of the present invention can be in any form. Theseforms include, but are not limited to, solutions, suspensions,dispersions, ointments (including oral ointments), creams, pastes, gels,powders (including tooth powders), toothpastes, lozenges, salve, chewinggum, mouth sprays, pastilles, sachets, mouthwashes, aerosols, tablets,capsules, transdermal patches, that comprise one or more resolvinsand/or protectins or their analogues of the invention.

Formulations of a composition comprising a recombinant human MIS proteinor functional fragment or variant thereof as disclosed herein can beprepared by a number or means known to persons skilled in the art. Insome embodiments the formulations can be prepared for administration asan aerosol formulation, e.g., by combining (i) a recombinant human MISprotein or functional fragment or variant thereof as disclosed herein inan amount sufficient to provide a plurality of therapeutically effectivedoses; (ii) the water addition in an amount effective to stabilize eachof the formulations; (iii) the propellant in an amount sufficient topropel a plurality of doses from an aerosol canister; and (iv) anyfurther optional components e.g. ethanol as a cosolvent; and dispersingthe components. The components can be dispersed using a conventionalmixer or homogenizer, by shaking, or by ultrasonic energy. Bulkformulation can be transferred to smaller individual aerosol vials byusing valve to valve transfer methods, pressure filling or by usingconventional cold-fill methods. It is not required that a stabilizerused in a suspension aerosol formulation be soluble in the propellant.Those that are not sufficiently soluble can be coated onto the drugparticles in an appropriate amount and the coated particles can then beincorporated in a formulation as described above.

In certain embodiments, a composition comprising a recombinant human MISprotein as disclosed herein can be administered to a subject as apharmaceutical composition with a pharmaceutically acceptable carrier.In certain embodiments, these pharmaceutical compositions optionallyfurther comprise one or more additional therapeutic agents. In certainembodiments, the additional therapeutic agent or agents are autoimmunedisease or drugs, such as immune suppressants and the like. In someembodiments, an additional therapeutic agent is a cortiosteriod. In someembodiments, an additional therapeutic agent is selected from the groupconsisting of Prednisone, methylprednisolone, Kenalog, Medrol Oral,Medrol (Pak) Oral, Depo-Medrol Inj, prednisolone Oral, Solu-Medrol Inj,hydrocortisone Oral, Cortef Oral, Solu-Medrol IV, cortisone Oral,Celestone Soluspan Inj, Orapred ODT Oral, Orapred Oral, Prelone Oral,methylprednisolone acetate Inj, Prednisone Intensol Oral, betamethasoneacet & sod phos Inj, Veripred, Celestone Oral, methylprednisolone sodiumsucc IV, methylprednisolone sodium succ Inj, Millipred Oral, Solu-Medrol(PF) Inj, Solu-Cortef Inj, Aristospan Intra-Articular Inj,hydrocortisone sod succinate Inj, prednisolone sodium phosphate Oral,methylprednisolone sod suc(PF) IV, Solu-Medrol (PF) IV, triamcinolonehexacetonide Inj, A-Hydrocort Inj, A-Methapred Inj, Millipred DP Oral,Flo-Pred Oral, Aristospan Intralesional Inj, betamethasone Oral,methylprednisolone sod suc(PF) Inj, hydrocortisone sod succ (PF) Inj,Solu-Cortef (PF) Inj, prednisolone acetate Oral, dexamethasone in 0.9%NaCl IV, Rayos, levothyroxine. Of course, such therapeutic agents arewhich are known to those of ordinary skill in the art can readily besubstituted as this list should not be considered exhaustive orlimiting.

Wetting agents, emulsifiers and lubricants, such as sodium laurylsulfate and magnesium stearate, as well as coloring agents, releaseagents, coating agents, sweetening, flavoring and perfuming agents,preservatives and antioxidants can also be present in the compositions.Examples of pharmaceutically acceptable antioxidants include: watersoluble antioxidants, such as ascorbic acid, cysteine hydrochloride,sodium bisulfate, sodium metabisulfate, sodium sulfite and the like;oil-soluble antioxidants, such as ascorbyl palmitate, butylatedhydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propylgallate, alpha-tocopherol, and the like; and metal chelating agents,such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol,tartaric acid, phosphoric acid, and the like.

Formulations of the present invention include those suitable forintravenous, oral, nasal, topical, transdermal, buccal, sublingual,rectal, vaginal and/or parenteral administration. The formulations mayconveniently be presented in unit dosage form and may be prepared by anymethods well known in the art of pharmacy. The amount of activeingredient which can be combined with a carrier material to produce asingle dosage form will generally be that amount of the compound whichproduces a therapeutic effect. Generally, out of one hundred percent,this amount will range from about 1 percent to about ninety-nine percentof active ingredient, preferably from about 5 percent to about 70percent, most preferably from about 10 percent to about 30 percent.

Formulations of the invention suitable for oral administration may be inthe form of capsules, cachets, pills, tablets, lozenges (using aflavored basis, usually sucrose and acacia or tragacanth), powders,granules, or as a solution or a suspension in an aqueous or non-aqueousliquid, or as an oil-in-water or water-in-oil liquid emulsion, or as anelixir or syrup, or as pastilles (using an inert base, such as gelatinand glycerin, or sucrose and acacia) and/or as mouth washes and thelike, each containing a predetermined amount of a compound of thepresent invention as an active ingredient. A compound of the presentinvention may also be administered as a bolus, electuary or paste.

In solid dosage forms of the invention for oral administration(capsules, tablets, pills, dragees, powders, granules and the like), theactive ingredient is mixed with one or more pharmaceutically acceptablecarriers, such as sodium citrate or dicalcium phosphate, and/or any ofthe following: fillers or extenders, such as starches, lactose, sucrose,glucose, mannitol, and/or silicic acid; binders, such as, for example,carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone,sucrose and/or acacia; humectants, such as glycerol; disintegratingagents, such as agar-agar, calcium carbonate, potato or tapioca starch,alginic acid, certain silicates, and sodium carbonate; solutionretarding agents, such as paraffin; absorption accelerators, such asquaternary ammonium compounds; wetting agents, such as, for example,cetyl alcohol and glycerol monostearate; absorbents, such as kaolin andbentonite clay; lubricants, such a talc, calcium stearate, magnesiumstearate, solid polyethylene glycols, sodium lauryl sulfate, andmixtures thereof; and coloring agents. In the case of capsules, tabletsand pills, the pharmaceutical compositions may also comprise bufferingagents. Solid compositions of a similar type may also be employed asfillers in soft and hard-filled gelatin capsules using such excipientsas lactose or milk sugars, as well as high molecular weight polyethyleneglycols and the like.

A tablet may be made by compression or molding, optionally with one ormore accessory ingredients. Compressed tablets may be prepared usingbinder (for example, gelatin or hydroxypropylmethyl cellulose),lubricant, inert diluent, preservative, disintegrant (for example,sodium starch glycolate or cross-linked sodium carboxymethyl cellulose),surface-active or dispersing agent. Molded tablets may be made bymolding in a suitable machine a mixture of the powdered compoundmoistened with an inert liquid diluent.

The tablets, and other solid dosage forms of the pharmaceuticalcompositions of the present invention, such as dragees, capsules, pillsand granules, may optionally be scored or prepared with coatings andshells, such as enteric coatings and other coatings well known in thepharmaceutical-formulating art. They may also be formulated so as toprovide slow or controlled release of the active ingredient thereinusing, for example, hydroxypropylmethyl cellulose in varying proportionsto provide the desired release profile, other polymer matrices,liposomes and/or microspheres. They may be sterilized by, for example,filtration through a bacteria-retaining filter, or by incorporatingsterilizing agents in the form of sterile solid compositions which canbe dissolved in sterile water, or some other sterile injectable mediumimmediately before use. These compositions may also optionally containopacifying agents and may be of a composition that they release theactive ingredient(s) only, or preferentially, in a certain portion ofthe gastrointestinal tract, optionally, in a delayed manner. Examples ofembedding compositions which can be used include polymeric substancesand waxes. The active ingredient can also be in micro-encapsulated form,if appropriate, with one or more of the above-described excipients.

Liquid dosage forms for oral administration of the compounds of theinvention include pharmaceutically acceptable emulsions, microemulsions,solutions, suspensions, syrups and elixirs.

In addition to the active ingredient, the liquid dosage forms maycontain inert diluents commonly used in the art, such as, for example,water or other solvents, solubilizing agents and emulsifiers, such asethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzylalcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils(in particular, cottonseed, groundnut, corn, germ, olive, castor andsesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycolsand fatty acid esters of sorbitan, and mixtures thereof. Besides inertdiluents, the oral compositions can also include adjuvants such aswetting agents, emulsifying and suspending agents, sweetening,flavoring, coloring, perfuming and preservative agents.

Suspensions, in addition to the active compounds, may contain suspendingagents as, for example, ethoxylated isostearyl alcohols, polyoxyethylenesorbitol and sorbitan esters, microcrystalline cellulose, aluminummetahydroxide, bentonite, agar-agar and tragacanth, and mixturesthereof.

In some instances, a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereincan be in a formulation suitable for rectal or vaginal administration,for example as a suppository, which may be prepared by mixing one ormore compounds of the invention with one or more suitable nonirritatingexcipients or carriers comprising, for example, cocoa butter,polyethylene glycol, a suppository wax or a salicylate, and which issolid at room temperature, but liquid at body temperature and, thereforerelease the active compound. Suitable carriers and formulations for suchadministration are known in the art.

Dosage forms for the topical or transdermal administration of arecombinant human MIS protein of this invention, e.g., for muscularadministration include powders, sprays, ointments, pastes, creams,lotions, gels, solutions, patches and inhalants. A recombinant human MISprotein or functional fragment or variant thereof as disclosed hereinmay be mixed under sterile conditions with a pharmaceutically acceptablecarrier, and with any preservatives, buffers, or propellants which maybe required.

The ointments, pastes, creams and gels may contain, in addition to anactive compound of this invention, excipients, such as animal andvegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulosederivatives, polyethylene glycols, silicones, bentonites, silicic acid,talc and zinc oxide, or mixtures thereof. Powders and sprays cancontain, in addition to a compound of this invention, excipients such aslactose, talc, silicic acid, aluminum hydroxide, calcium silicates andpolyamide powder, or mixtures of these substances. Sprays canadditionally contain customary propellants, such aschlorofluorohydrocarbons and volatile unsubstituted hydrocarbons, suchas butane and propane.

Transdermal patches have the added advantage of providing controlleddelivery of a recombinant human MIS protein of the present invention tothe body. Such dosage forms can be made by dissolving or dispersing thecompound in the proper medium. Absorption enhancers can also be used toincrease the flux of the compound across the skin. The rate of such fluxcan be controlled by either providing a rate controlling membrane ordispersing the active compound in a polymer matrix or gel.

Pharmaceutical compositions of this invention suitable for parenteraladministration comprise one or more compounds of the invention incombination with one or more pharmaceutically acceptable sterileisotonic aqueous or nonaqueous solutions, dispersions, suspensions oremulsions, or sterile powders which may be reconstituted into sterileinjectable solutions or dispersions just prior to use, which may containantioxidants, buffers, bacteriostats, solutes which render theformulation isotonic with the blood of the intended recipient orsuspending or thickening agents.

Examples of suitable aqueous and nonaqueous carriers which may beemployed in the pharmaceutical compositions of the invention includewater, ethanol, polyols (such as glycerol, propylene glycol,polyethylene glycol, and the like), and suitable mixtures thereof,vegetable oils, such as olive oil, and injectable organic esters, suchas ethyl oleate. Proper fluidity can be maintained, for example, by theuse of coating materials, such as lecithin, by the maintenance of therequired particle size in the case of dispersions, and by the use ofsurfactants.

These compositions may also contain adjuvants such as preservatives,wetting agents, emulsifying agents and dispersing agents. Prevention ofthe action of microorganisms may be ensured by the inclusion of variousantibacterial and antifungal agents, for example, paraben,chlorobutanol, phenol sorbic acid, and the like. It may also bedesirable to include isotonic agents, such as sugars, sodium chloride,and the like into the compositions. In addition, prolonged absorption ofthe injectable pharmaceutical form may be brought about by the inclusionof agents which delay absorption such as aluminum monostearate andgelatin.

In some cases, in order to prolong the effect of a drug, it is desirableto slow the absorption of the drug from subcutaneous or intramuscularinjection. This may be accomplished by the use of a liquid suspension ofcrystalline or amorphous material having poor water solubility. The rateof absorption of the drug then depends upon its rate of dissolutionwhich, in turn, may depend upon crystal size and crystalline form.Alternatively, delayed absorption of a parenterally-administered drugform is accomplished by dissolving or suspending the drug in an oilvehicle.

Injectable depot forms are made by forming microencapsulated matrices ofthe subject compounds in biodegradable polymers such aspolylactide-polyglycolide. Depending on the ratio of drug to polymer,and the nature of the particular polymer employed, the rate of drugrelease can be controlled. Examples of other biodegradable polymersinclude poly(orthoesters) and poly(anhydrides). Depot injectableformulations are also prepared by entrapping the drug in liposomes ormicroemulsions which are compatible with body tissue.

In certain embodiments, a recombinant human MIS protein or functionalfragment or variant thereof can be isolated and/or purified orsubstantially purified by one or more purification methods describedherein or known by those skilled in the art. Generally, the purities areat least 90%, in particular 95% and often greater than 99%. In certainembodiments, the naturally occurring compound is excluded from thegeneral description of the broader genus.

In some embodiments, the composition comprises at least one arecombinant human MIS protein in combination with a pharmaceuticallyacceptable carrier. Some examples of materials which can serve aspharmaceutically acceptable carriers include, without limitation:sugars, such as lactose, glucose and sucrose; starches, such as cornstarch and potato starch; cellulose, and its derivatives, such as sodiumcarboxymethyl cellulose, ethyl cellulose and cellulose acetate; powderedtragacanth; malt; gelatin; talc; excipients, such as cocoa butter andsuppository waxes; oils, such as peanut oil, cottonseed oil, saffloweroil, sesame oil, olive oil, corn oil and soybean oil; glycols, such aspropylene glycol; polyols, such as glycerin, sorbitol, mannitol andpolyethylene glycol; esters, such as ethyl oleate and ethyl laurate;agar; buffering agents, such as magnesium hydroxide and aluminumhydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer'ssolution; ethyl alcohol; phosphate buffer solutions; and other non-toxiccompatible substances employed in pharmaceutical formulations.

In certain embodiments, a composition comprising a recombinant human MISprotein or functional fragment or variant thereof as disclosed hereincan contain one or more acidic functional groups and, thus, are capableof forming pharmaceutically acceptable salts with pharmaceuticallyacceptable bases. The term “pharmaceutically acceptable salts, esters,amides, and prodrugs” as used herein refers to those carboxylate salts,amino acid addition salts, esters, amides, and prodrugs of the compoundsof the present invention which are, within the scope of sound medicaljudgment, suitable for use in contact with the tissues of patientswithout undue toxicity, irritation, allergic response, and the like,commensurate with a reasonable benefit/risk ratio, and effective fortheir intended use of the compounds of the invention. The term “salts”refers to the relatively non-toxic, inorganic and organic acid additionsalts of compounds of the present invention.

These salts can be prepared in situ during the final isolation andpurification of the compounds or by separately reacting the purifiedcompound in its free base form with a suitable organic or inorganic acidand isolating the salt thus formed. These may include cations based onthe alkali and alkaline earth metals, such as sodium, lithium,potassium, calcium, magnesium and the like, as well as non-toxicammonium, quaternary ammonium, and amine cations including, but notlimited to ammonium, tetramethylammonium, tetraethylammonium,methylamine, dimethylamine, trimethylamine, triethylamine, ethylamine,and the like. (See, for example, Berge S. M., et al., “PharmaceuticalSalts,” J. Pharm. Sci., 1977; 66:1-19 which is incorporated herein byreference).

The term “pharmaceutically acceptable esters” refers to the relativelynon-toxic, esterified products of the compounds of the presentinvention. These esters can be prepared in situ during the finalisolation and purification of the compounds, or by separately reactingthe purified compound in its free acid form or hydroxyl with a suitableesterifying agent. Carboxylic acids can be converted into esters viatreatment with an alcohol in the presence of a catalyst. The term isfurther intended to include lower hydrocarbon groups capable of beingsolvated under physiological conditions, e.g., alkyl esters, methyl,ethyl and propyl esters.

As used herein, “pharmaceutically acceptable salts or prodrugs” aresalts or prodrugs that are, within the scope of sound medical judgment,suitable for use in contact with the tissues of patients without unduetoxicity, irritation, allergic response, and the like, commensurate witha reasonable benefit/risk ratio, and effective for their intended use.These compounds include the zwitterionic forms, where possible, of rcompounds of the invention.

The term “salts” refers to the relatively non-toxic, inorganic andorganic acid addition salts of compounds of the present invention. Thesesalts can be prepared in situ during the final isolation andpurification of the compounds or by separately reacting the purifiedcompound in its free base form with a suitable organic or inorganic acidand isolating the salt thus formed. These may include cations based onthe alkali and alkaline earth metals, such as sodium, lithium,potassium, calcium, magnesium and the like, as well as non-toxicammonium, quaternary ammonium, and amine cations including, but notlimited to ammonium, tetramethylanunonium, tetraethyl ammonium, methylamine, dimethyl amine, trimethylamine, triethylamine, ethylamine, andthe like (see, e.g., Berge S. M., et al. (1977) J. Pharm. Sci. 66, 1,which is incorporated herein by reference).

The term “prodrug” refers to compounds or agents that are rapidlytransformed in vivo to yield the active recombinant human MIS protein,e.g., a biologically active or functional active MIS protein or nucleicacid (e.g., mRNA, DNA, MOD-RNA) which encodes a functionally active MISprotein. In some embodiments, a recombinant human MIS protein prodrugcan be activated by hydrolysis in blood, e.g., via cleavage of a leadersequence, and or cleavage at the primary cleavage site to result in theN-terminal and C-terminal domains for production of a bioactive MISprotein, similar to how insulin is activated from its proprotein into anactive insulin protein. A thorough discussion is provided in T. Higachiand V. Stella, “Pro-drugs as Novel Delivery Systems,” Vol. 14 of theA.C.S. Symposium Series, and in Bioreversible Carriers in: Drug Design,ed. Edward B. Roche, American Pharmaceutical Association and PergamonPress, 1987, both of which are hereby incorporated by reference. As usedherein, a prodrug is a compound that, upon in vivo administration, ismetabolized or otherwise converted to the biologically, pharmaceuticallyor therapeutically active form of the compound. The prodrug may bedesigned to alter the metabolic stability or the transportcharacteristics of a recombinant human MIS protein, to mask side effectsor toxicity, or to alter other characteristics or properties of therecombinant human MIS protein.

By virtue of knowledge of pharmacodynamic processes and drug metabolismor post-translational protein processing of MIS in vivo, once apharmaceutically active compound is identified, those of skill in thepharmaceutical art generally can design a recombinant human MIS proteinprodrug which can be activated in vivo to increase levels of a bioactiveMIS protein in the subject (see, e.g., Nogrady (1985) MedicinalChemistry A Biochemical Approach, Oxford University Press, N.Y., pages388-392). Conventional procedures for the selection and preparation ofsuitable prodrugs are described, for example, in “Design of Prodrugs,”ed. H. Bundgaard, Elsevier, 1985. Suitable examples of prodrugs includemethyl, ethyl and glycerol esters of the corresponding acid.

As discussed herein, in some embodiments a composition comprising arecombinant human MIS protein or functional fragment or variant thereofas disclosed herein can be conjugated or covalently attached to atargeting agent to increase their tissue specificity and targeting to acell, for example a muscle cells. Targeting agents can include, forexample without limitation, antibodies, cytokines and receptor ligands,as discussed in the section entitled “targeting.” In some embodiments,the targeting agent is overexpressed on the cells to be targeted, forexample the muscle cells as compared to non-muscle cells.

Regardless of the route of administration selected, the compounds of thepresent invention, which may be used in a suitable hydrated form, and/orthe pharmaceutical compositions of the present invention, are formulatedinto pharmaceutically acceptable dosage forms by conventional methodsknown to those of ordinary skill in the art.

Gene Therapy

In some embodiments, a nucleic acid encoding a recombinant human MISprotein or functional fragment thereof as disclosed herein, can besuitably administered as a vector, e.g., a viral vector.

In some embodiments, a nucleic acid encoding a recombinant human MISprotein can be effectively used in treatment by gene therapy. See,generally, for example, U.S. Pat. No. 5,399,346, which is incorporatedherein by reference. The general principle is to introduce thepolynucleotide into a target cell in a patient, and where it istranscribed into the protein.

Entry into the cell can be facilitated by suitable techniques known inthe art such as providing the polynucleotide in the form of a suitablevector, or encapsulation of the polynucleotide in a liposome.

A desired mode of gene therapy is to provide the polynucleotide in sucha way that it will replicate inside the cell, enhancing and prolongingthe desired effect. Thus, the polynucleotide is operably linked to asuitable promoter, such as the natural promoter of the correspondinggene, a heterologous promoter that is intrinsically active in liver,neuronal, bone, muscle, skin, joint, or cartilage cells, or aheterologous promoter that can be induced by a suitable agent.

Expression vectors compatible with eukaryotic cells, preferably thosecompatible with vertebrate cells, can be used to produce recombinantconstructs for the expression of a recombinant human MIS protein or afunctional derivative or functional variant or functional fragmentthereof as disclosed herein. Eukaryotic cell expression vectors are wellknown in the art and are available from several commercial sources.Typically, such vectors are provided containing convenient restrictionsites for insertion of the desired DNA segment. These vectors can beviral vectors such as adenovirus, adeno-associated virus, pox virus suchas an orthopox (vaccinia and attenuated vaccinia), avipox, lentivirus,murine moloney leukemia virus, etc.

Alternatively, in some embodiments, a plasmid expression vector can beused. Plasmid expression vectors include, but are not limited to,pcDNA3.1, pET vectors (Novagen 0), pGEX vectors (GE Life Sciences), andpMAL vectors (New England labs. Inc.) for protein expression in E. colihost cell such as BL21, BL21(DE3) and AD494(DE3)pLysS, Rosetta (DE3),and Origami(DE3) ((Novagen 0); the strong CMV promoter-based pcDNA3.1(Invitrogen™ Inc.) and pCIneo vectors (Promega) for expression inmammalian cell lines such as CHO, COS, HEK-293, Jurkat, and MCF-7;replication incompetent adenoviral vector vectors pAdeno X, pAd5F35,pLP-Adeno-X-CMV (Clontech 0), pAd/CMV/V5-DEST, pAd-DEST vector(Invitrogen™ Inc.) for adenovirus-mediated gene transfer and expressionin mammalian cells; pLNCX2, pLXSN, and pLAPSN retrovirus vectors for usewith the Retro-X™ system from Clontech for retroviral-mediated genetransfer and expression in mammalian cells; pLenti4N5-DESTTm,pLenti6N5-DESTTm, and pLenti6.2N5-GW/lacZ (INVITROGEN™ Inc.) forlentivirus-mediated gene transfer and expression in mammalian cells;adenovirus-associated virus expression vectors such as pAAV-MC andpAAV-IRES-hrGFP for adeno-associated virus-mediated gene transfer andexpression in mammalian cells; BACpak6 baculovirus (Clontech®) andpFastBac™ HT (Invitrogen™ Inc.) for the expression in Spodoperafrugiperda 9 (Sf9) and Sf11 insect cell lines; pMT/BiPN5-His(Invitrogen™ Inc.) for the expression in Drosophila Schneider S2 cells;Pichia expression vectors pPICZα, pPICZ, pFLDα and pFLD (Invitrogen™Inc.) for expression in Pichia pastoris and vectors pMETα and pMET forexpression in P. methanolica; pYES2/GS and pYD1 (Invitrogen™ Inc.)vectors for expression in yeast Saccharomyces cerevisiae. Recentadvances in the large scale expression heterologous proteins inChlamydomonas reinhardtii are described by Griesbeck C. et. al. 2006Mol. Biotechnol. 34:213-33 and Fuhrmann M. 2004, Methods Mol Med.94:191-5. Foreign heterologous coding sequences are inserted into thegenome of the nucleus, chloroplast and mitochodria by homologousrecombination. The chloroplast expression vector p64 carrying the mostversatile chloroplast selectable marker aminoglycoside adenyltransferase (aadA), which confer resistance to spectinomycin orstreptomycin, can be used to express foreign protein in the chloroplast.Biolistic gene gun method is used to introduced the vector in the algae.Upon its entry into chloroplasts, the foreign DNA is released from thegene gun particles and integrates into the chloroplast genome throughhomologous recombination.

Viral vector systems which can be utilized in the present inventioninclude, but are not limited to, (a) adenovirus vectors; (b) retrovirusvectors; (c) adeno-associated virus vectors; (d) herpes simplex virusvectors; (e) SV 40 vectors; (f) polyoma virus vectors; (g) papillomavirus vectors; (h) picornavirus vectors; (i) pox virus vectors such asan orthopox, e.g., vaccinia virus vectors or avipox, e.g. canary pox orfowl pox; and (j) a helper-dependent or gutless adenovirus. In apreferred embodiment, the vector is an adenovirus. Replication-defectiveviruses can also be advantageous.

The vector may or may not be incorporated into the cells genome. Theconstructs may include viral sequences for transfection, if desired.Alternatively, the construct may be incorporated into vectors capable ofepisomal replication, e.g., EPV and EBV vectors.

Constructs for the expression of a nucleic acid encoding a recombinanthuman MIS protein as disclosed herein., e.g., DNA, MOD-RNA or RNAa, cangenerally be operatively linked to regulatory elements, e.g., promoters,enhancers, etc., to ensure the expression of the construct in targetcells. Other specifics for vectors and constructs are described infurther detail below.

Typical regulatory sequences include, but are not limited to,transcriptional promoters, inducible promoters and transcriptionalelements, an optional operate sequence to control transcription, asequence encoding suitable mRNA ribosomal binding sites, and sequencesto control the termination of transcription and/or translation. Includedin the term “regulatory elements” are nucleic acid sequences such asinitiation signals, enhancers, and promoters, which induce or controltranscription of protein coding sequences with which they areoperatively linked. In some examples, transcription of a recombinantgene is under the control of a promoter sequence (or othertranscriptional regulatory sequence) which controls the expression ofthe recombinant gene in a cell-type in which expression is intended. Itwill also be understood that the recombinant gene can be under thecontrol of transcriptional regulatory sequences which are the same orwhich are different from those sequences which control transcription ofthe naturally-occurring form of a protein. In some instances thepromoter sequence is recognized by the synthetic machinery of the cell,or introduced synthetic machinery, required for initiating transcriptionof a specific gene.

Regulatory sequences can be a single regulatory sequence or multipleregulatory sequences, or modified regulatory sequences or fragmentsthereof. Modified regulatory sequences are regulatory sequences wherethe nucleic acid sequence has been changed or modified by some means,for example, but not limited to, mutation, methylation etc. Regulatorysequences useful in the methods as disclosed herein are promoterelements which are sufficient to render promoter-dependent geneexpression controllable for cell type-specific, tissue-specific orinducible by external signals or agents (e g enhancers or repressors);such elements may be located in the 5′ or 3′ regions of the native gene,or within an intron.

As used herein, the term “tissue-specific promoter” means a nucleic acidsequence that serves as a promoter, i.e., regulates expression of aselected nucleic acid sequence operably linked to the promoter, andwhich selectively affects expression of the selected nucleic acidsequence in specific cells of a tissue, such as cells of ovarian origin.

The term “constitutively active promoter” refers to a promoter of a genewhich is expressed at all times within a given cell. Exemplary promotersfor use in mammalian cells include cytomegalovirus (CMV), and for use inprokaryotic cells include the bacteriophage T7 and T3 promoters, and thelike. The term “inducible promoter” refers to a promoter of a gene whichcan be expressed in response to a given signal, for example addition orreduction of an agent. Non-limiting examples of an inducible promoterare “tet-on” and “tet-off” promoters, or promoters that are regulated ina specific tissue type.

In a specific embodiment, viral vectors that contain nucleic acidsequences e.g., DNA, MOD-RNA or RNAa encoding a recombinant human MISprotein or functional fragment thereof as disclosed herein can be used.For example, a retroviral vector can be used (see Miller et al., Meth.Enzymol. 217:581-599 (1993)). These retroviral vectors contain thecomponents necessary for the correct packaging of the viral genome andintegration into the host cell DNA. The nucleic acid sequences encodinga recombinant human MIS protein are cloned into one or more vectors,which facilitates delivery of the gene into a patient. More detail aboutretroviral vectors can be found in Boesen et al., Biotherapy 6:291-302(1994), which describes the use of a retroviral vector to deliver themdrl gene to hematopoietic stem cells in order to make the stem cellsmore resistant to chemotherapy. Other references illustrating the use ofretroviral vectors in gene therapy are: Clowes et al., J. Clin. Invest.93:644-651 (1994); Kiem et al., Blood 83:1467-1473 (1994); Salmons andGunzberg, Human Gene Therapy 4:129-141 (1993); and Grossman and Wilson,Curr. Opin. in Genetics and Devel. 3:110-114 (1993).

The production of a recombinant retroviral vector carrying a gene ofinterest is typically achieved in two stages. First, sequence encoding arecombinant human MIS protein or a functional derivative or functionalvariant or functional fragment thereof, alone or fused to −Fc can beinserted into a retroviral vector which contains the sequences necessaryfor the efficient expression of the metabolic regulators (includingpromoter and/or enhancer elements which can be provided by the virallong terminal repeats (LTRs) or by an internal promoter/enhancer andrelevant splicing signals), sequences required for the efficientpackaging of the viral RNA into infectious virions (e.g., a packagingsignal (Psi), a tRNA primer binding site (−PBS), a 3′ regulatorysequence required for reverse transcription (+PBS)), and a viral LTRs).The LTRs contain sequences required for the association of viral genomicRNA, reverse transcriptase and integrase functions, and sequencesinvolved in directing the expression of the genomic RNA to be packagedin viral particles.

Following the construction of the recombinant retroviral vector, thevector DNA is introduced into a packaging cell line. Packaging celllines provide viral proteins required in trans for the packaging ofviral genomic RNA into viral particles having the desired host range(e.g., the viral-encoded core (gag), polymerase (pol) and envelope (env)proteins). The host range is controlled, in part, by the type ofenvelope gene product expressed on the surface of the viral particle.Packaging cell lines can express ecotrophic, amphotropic or xenotropicenvelope gene products. Alternatively, the packaging cell line can lacksequences encoding a viral envelope (env) protein. In this case, thepackaging cell line can package the viral genome into particles whichlack a membrane-associated protein (e.g., an env protein). To produceviral particles containing a membrane-associated protein which permitsentry of the virus into a cell, the packaging cell line containing theretroviral sequences can be transfected with sequences encoding amembrane-associated protein (e.g., the G protein of vesicular stomatitisvirus (VSV)). The transfected packaging cell can then produce viralparticles which contain the membrane-associated protein expressed by thetransfected packaging cell line; these viral particles which containviral genomic RNA derived from one virus encapsidated by the envelopeproteins of another virus are said to be pseudotyped virus particles.

Adenoviruses are other viral vectors that can be used in gene therapy.Adenoviruses are especially attractive vehicles for delivering genes torespiratory epithelia. Adenoviruses naturally infect respiratoryepithelia where they cause a mild disease. Other targets foradenovirus-based delivery systems are liver, the central nervous system,endothelial cells, and muscle. Adenoviruses have the advantage of beingcapable of infecting non-dividing cells. Kozarsky and Wilson, CurrentOpinion in Genetics and Development 3:499-503 (1993) present a review ofadenovirus-based gene therapy. Bout et al., Human Gene Therapy 5:3-10(1994) demonstrated the use of adenovirus vectors to transfer genes tothe respiratory epithelia of rhesus monkeys. Another preferred viralvector is a pox virus such as a vaccinia virus, for example anattenuated vaccinia such as Modified Virus Ankara (MVA) or NYVAC, anavipox such as fowl pox or canary pox. Other instances of the use ofadenoviruses in gene therapy can be found in Rosenfeld et al., Science252:431-434 (1991); Rosenfeld et al., Cell 68:143-155 (1992);Mastrangeli et al., J. Clin. Invest. 91:225-234 (1993); PCT PublicationWO94/12649; and Wang, et al., Gene Therapy 2:775-783 (1995). In anotherembodiment, lentiviral vectors are used, such as the HIV based vectorsdescribed in U.S. Pat. Nos. 6,143,520; 5,665,557; and 5,981,276, whichare herein incorporated by reference. In some embodiments, a viralvector such as an Adeno-associated virus (AAV) vector is used. ExemplaryAAV vectors are disclosed in Walsh et al., Proc. Soc. Exp. Biol. Med.204:289-300 (1993); U.S. Pat. No. 5,436,146 which is incorporated hereinby reference; Gao et al., Gene Therapy 2005, 5, 285-297; Vandenberghe etal., Gene Therapy 2009, 16, 311-319; Gao et al., PNAS 2002, 99,11854-11859; Gao et al., PNAS 2003, 100, 6081-6086; Gao et al., J. ofVirology 2004, 78, 6381-6388; Molecular Cloning: A Laboratory Manual(4^(th) edition) ed. by M. Green and J. Sambrook. In some embodiments,the AAV vector is an AAV1, AAV2, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9,AAVrh.10, AAV2.5. It should be noted that the selection of a particulartype of AAV vectors can depend on the target tissue.

In some embodiments, when a recombinant human MIS protein encoded by aviral vector is expressed endogenously in a subject, the expressionlevel of the recombinant human MIS protein disclosed herein can beconstant over a desired period of time, for example, at least 1 week, atleast 2 weeks, at least 3 weeks, at least 1 month, at least 2 months, atleast 3 months, at least 6 months, at least 1 year, or at least 5 years.In some embodiments, the expression of the recombination human MISprotein disclosed herein can be sustained at or above a therapeuticallyeffective dosage level over a desired period of time.

Another approach to gene therapy involves transferring a gene to cellsin tissue culture by such methods as electroporation, lipofection,calcium phosphate mediated transfection, or viral infection. Usually,the method of transfer includes the transfer of a selectable marker tothe cells. The cells are then placed under selection to isolate thosecells that have taken up and are expressing the transferred gene. Thosecells are then delivered to a patient.

U.S. Pat. No. 5,676,954 (which is herein incorporated by reference)reports on the injection of genetic material, complexed with cationicliposome carriers, into mice. U.S. Pat. Nos. 4,897,355, 4,946,787,5,049,386, 5,459,127, 5,589,466, 5,693,622, 5,580,859, 5,703,055, andinternational publication NO: WO 94/9469 (which are herein incorporatedby reference) provide cationic lipids for use in transfecting DNA intocells and mammals. U.S. Pat. Nos. 5,589,466, 5,693,622, 5,580,859,5,703,055, and international publication NO: WO 94/9469 (which areherein incorporated by reference) provide methods for deliveringDNA-cationic lipid complexes to mammals. Such cationic lipid complexesor nanoparticles can also be used to deliver protein.

A gene or nucleic acid sequence can be introduced into a target cell byany suitable method. For example, a recombinant human MIS proteinconstruct can be introduced into a cell by transfection (e.g., calciumphosphate or DEAE-dextran mediated transfection), lipofection,electroporation, microinjection (e.g., by direct injection of nakedDNA), biolistics, infection with a viral vector containing a musclerelated transgene, cell fusion, chromosome-mediated gene transfer,microcell-mediated gene transfer, nuclear transfer, and the like. Anucleic acid encoding a recombinant human MIS protein can be introducedinto cells by electroporation (see, e.g., Wong and Neumann, Biochem.Biophys. Res. Commun. 107:584-87 (1982)) and biolistics (e.g., a genegun; Johnston and Tang, Methods Cell Biol. 43 Pt A:353-65 (1994); Fynanet al., Proc. Natl. Acad. Sci. USA 90:11478-82 (1993)).

In certain embodiments, a gene or nucleic acid sequence encoding arecombinant human MIS protein can be introduced into target cells bytransfection or lipofection. Suitable agents for transfection orlipofection include, for example, calcium phosphate, DEAE dextran,lipofectin, lipfectamine, DIMRIE C, Superfect, and Effectin (Qiagen),unifectin, maxifectin, DOTMA, DOGS (Transfectam;dioctadecylamidoglycylspermine), DOPE(1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), DOTAP(1,2-dioleoyl-3-trimethylammonium propane), DDAB (dimethyldioctadecylammonium bromide), DHDEAB(N,N-di-n-hexadecyl-N,N-dihydroxyethyl ammonium bromide), HDEAB(N-n-hexadecyl-N,N-dihydroxyethylammonium bromide), polybrene,poly(ethylenimine) (PEI), and the like. (See, e.g., Banerjee et al.,Med. Chem. 42:4292-99 (1999); Godbey et al., Gene Ther. 6:1380-88(1999); Kichler et al., Gene Ther. 5:855-60 (1998); Birchaa et al., J.Pharm. 183:195-207 (1999)).

Methods known in the art for the therapeutic delivery of agents such asproteins and/or nucleic acids can be used for the delivery of apolypeptide or nucleic acid encoding a recombinant human MIS protein toa subject, e.g., cellular transfection, gene therapy, directadministration with a delivery vehicle or pharmaceutically acceptablecarrier, indirect delivery by providing recombinant cells comprising anucleic acid encoding a targeting fusion polypeptide of the invention.

Various delivery systems are known and can be used to directlyadminister therapeutic polypeptides such as a recombinant human MISprotein and/or a nucleic acid encoding a recombinant human MIS proteinas disclosed herein, e.g., encapsulation in liposomes, microparticles,microcapsules, recombinant cells capable of expressing the compound, andreceptor-mediated endocytosis (see, e.g., Wu and Wu, 1987, J. Biol.Chem. 262:4429-4432). Methods of introduction can be enteral orparenteral and include but are not limited to intradermal,intramuscular, intraperitoneal, intravenous, subcutaneous, pulmonary,intranasal, intraocular, epidural, and oral routes. The agents may beadministered by any convenient route, for example by infusion or bolusinjection, by absorption through epithelial or mucocutaneous linings(e.g., oral mucosa, rectal and intestinal mucosa, etc.) and may beadministered together with other biologically active agents.Administration can be systemic or local.

In a specific embodiment, it may be desirable to administer thepharmaceutical compositions of the invention locally to the area in needof treatment; this may be achieved, for example, and not by way oflimitation, by local infusion during surgery, topical application, e.g.,by injection, by means of a catheter, or by means of an implant, theimplant being of a porous, non-porous, or gelatinous material, includingmembranes, such as sialastic membranes, fibers, or commercial skinsubstitutes.

In another embodiment, the active agent can be delivered in a vesicle,in particular a liposome (see Langer (1990) Science 249:1527-1533). Inyet another embodiment, the active agent can be delivered in acontrolled release system. In one embodiment, a pump may be used (seeLanger (1990) supra). In another embodiment, polymeric materials can beused (see Howard et al. (1989) J. Neurosurg. 71:105).

Thus, a wide variety of gene transfer/gene therapy vectors andconstructs are known in the art. These vectors are readily adapted foruse in the methods of the present invention. By the appropriatemanipulation using recombinant DNA/molecular biology techniques toinsert an operatively linked recombinant human MIS protein encodingnucleic acid segment into the selected expression/delivery vector, manyequivalent vectors for the practice of the methods described herein canbe generated.

It will be appreciated by those of skill that cloned genes readily canbe manipulated to alter the amino acid sequence of a protein. The clonedgene for recombinant human MIS protein can be manipulated by a varietyof well known techniques for in vitro mutagenesis, among others, toproduce variants of the naturally occurring human protein, hereinreferred to as muteins or variants or mutants of a recombinant human MISprotein, which may be used in accordance with the methods andcompositions described herein.

The variation in primary structure of muteins of a recombinant human MISprotein useful in the invention, for instance, may include deletions,additions and substitutions. The substitutions may be conservative ornon-conservative. The differences between the natural protein and themutein generally conserve desired properties, mitigate or eliminateundesired properties and add desired or new properties.

Remington's Pharmaceutical sciences Ed. Germany, Merk Publishing,Easton, Pa., 1 995 (the contents of which are hereby incorporated byreference), discloses various carriers used in formulatingpharmaceutical compositions and known techniques for the preparationthereof. Some examples of materials which can serve as pharmaceuticallyacceptable carriers include, but are not limited to, sugars such aslactose, glucose and sucrose; starches such as corn starch and potatostarch; cellulose and its derivatives such as sodium carboxymethylcellulose, ethyl cellulose and cellulose acetate; malt; gelatin; talc;excipients such as cocoa butter and: suppository waxes; oils such aspeanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; cornoil and soybean oil; glycols; such a propylene glycol; esters such asethyl oleate and ethyl laurate; agar; buffering agents such as magnesiumhydroxide and aluminum hydroxide; water; isotonic saline; Ringer'ssolution, ethyl alcohol, and phosphate buffer solutions, as well asother non-toxic compatible lubricants such as sodium lauryl sulfate andmagnesium sulfate, as well as coloring agents, releasing agents, coatingagents, sweetening, flavoring and perfuming agents, preservatives andantioxidants can also be present in the composition, according to thejudgment of the formulator.

Kits

The invention also provides kits or pharmaceutical packages thatcomprise a recombinant human MIS protein or functional variant orfunctional fragment or fusion protein thereof for use in the preventionand/or treatment of a proliferative disease or disorder, e.g., cancer ora neurodegenerative disease such as a motor neuron disease or disease ofexcess androgen as disclosed herein. The kit can comprise a recombinanthuman MIS protein composition as disclosed herein in the form of, forexample, tablets, capsules, or lyophilized powders, and can optionallyinclude instructions for using a recombinant human MIS protein for thetreatment of cancer, or a neurodegenerative disease such as a motorneuron disease, or disease associated with androgen dependency. Acomposition comprising a recombinant human MIS protein or functionalvariant or functional fragment or fusion protein thereof can be providedin the kits or packages in a bottle or another appropriate form (e.g., ablister pack). Optionally, the kits or pharmaceutical packages can alsoinclude other pharmaceutically active agents (see, e.g., the agentslisted above, such as other agents used for treatment of autoimmunediseases and disorders), and/or materials used in administration of thedrug(s), such as diluents, needles, syringes, applicators, and the like.

Various embodiments of the disclosure could also include permutations ofthe various elements recited in the claims as if each dependent claimwas a multiple dependent claim incorporating the limitations of each ofthe preceding dependent claims as well as the independent claims. Suchpermutations are expressly within the scope of this disclosure.

While the invention has been particularly shown and described withreference to a number of embodiments, it would be understood by thoseskilled in the art that changes in the form and details may be made tothe various embodiments disclosed herein without departing from thespirit and scope of the invention and that the various embodimentsdisclosed herein are not intended to act as limitations on the scope ofthe claims. All references cited herein are incorporated in theirentirety by reference.

Each of the applications and patents cited in this text, as well as eachdocument or reference cited in each of the applications and patents(including during the prosecution of each issued patent; “applicationcited documents”), and each of the PCT and foreign applications orpatents corresponding to and/or claiming priority from any of theseapplications and patents, and each of the documents cited or referencedin each of the application cited documents, are hereby expresslyincorporated herein by reference and may be employed in the practice ofthe invention. More generally, documents or references are cited in thistext, either in a Reference List before the claims, or in the textitself; and, each of these documents or references (“herein citedreferences”), as well as each document or reference cited in each of theherein cited references (including any manufacturer's specifications,instructions, etc.), is hereby expressly incorporated herein byreference.

Some embodiments of the invention are listed in the followingparagraphs:

-   -   1. A method for treating a subject with a neurodegenerative        disease or disorder, comprising administering a composition        comprising a recombinant Mullerian Inhibiting Substance (MIS)        protein, wherein the recombinant MIS protein comprises a        modification of at least one amino acid between residues 448-452        of SEQ ID NO: 1.    -   2. The method of paragraph 1, wherein the modification increases        cleavage as compared to in the absence of the modification,        wherein the recombinant MIS protein has increased cleavage and        increased yield of production in vitro as compared to wild-type        MIS protein corresponding to amino acid residues of SEQ ID NO:        1.    -   3. The method of paragraph 1, wherein the recombinant MIS        protein is produced from a pre-proprotein comprising a non-MIS        leader sequence or a functional fragment thereof in place of the        MIS leader sequence of amino acids 1-25 of SEQ ID NO: 1.    -   4. The method of paragraph 1, wherein one or more neurons from        the subject express an MIS receptor.    -   5. The method of paragraph 4, wherein the MIS receptor is MIS        type II receptor or a homologue or functional fragment thereof.    -   6. The method of paragraph 1, wherein the neurodegenerative        disorder is a motor neuron disease.    -   7. The method of paragraph 6, wherein the motor neuron disease        is selected from the group of: amylotrophic lateral sclerosis        (ALS), progressive bulbar palsy, pseudobulbar palsy; primary        lateral sclerosis (PLS); progressive muscular atrophy; spinal        muscular atrophy (SMA, including SMA type I, SMA type II, and        SMA type III); Fazio-Londe disease; progressive spinobulbar        muscular atrophy; congenital SMA with arthrogryposis or        post-polio syndrome (PPS).    -   8. The method of paragraph 1, wherein the neurodegenerative        disease is selected from the group of: amyotrophic lateral        sclerosis (ALS), primary lateral sclerosis (PLS), spinal        muscular atrophy (SMA), Alzheimer's disease (AD), Parkinson's        disease (PD), Huntington's disease (HD), Friedreich's ataxia,        cerebellar ataxia, other brain disorders such as bipolar        disorder, epilepsy, schizophrenia, depression, mania, autism,        ADHD, brain trauma injuries and stroke.    -   9. The method of paragraph 1, wherein the recombinant MIS        protein further comprises a Tag protein.    -   10. The method of paragraph 3, wherein the non-MIS leader        sequence is an albumin leader sequence or a functional fragment        thereof.    -   11. The method of paragraph 10, wherein the albumin leader        sequence is a human serum albumin (HSA) leader sequence or a        fragment thereof.    -   12. The method of paragraph 11, wherein the HSA leader sequence        comprises the amino acid sequence of SEQ ID NO: 6 or a variant        that is at least 80% homologous thereto.    -   13. The method of paragraph 11, wherein a fragment of the HSA        leader sequence comprises at least 10 amino acids of SEQ ID NO:        6 or a variant that is at least 80% homologous thereto.    -   14. The method of paragraph 11, wherein the HSA leader sequence        comprises at least 15 amino acids of SEQ ID NO: 6, or a variant        that is at least 80% homologous thereto.    -   15. The method of paragraph 11, wherein the HSA leader sequence        comprises at least 11 amino acids of SEQ ID NO: 6, or a variant        that is at least 80% homologous thereto.    -   16. The method of paragraph 11, wherein a fragment of the HSA        leader sequence is selected from the group consisting of:        MKWVTFISLLFLFSSAYS (SEQ ID NO: 13); MKWVTFISLLFLFSSAYSRGVFRR        (SEQ ID NO: 6); MKWVSFISLLFLFSSAYS (SEQ ID NO:14).    -   17. The method of paragraph 3, wherein the non-MIS leader        sequence is selected from a group consisting of: immunoglobulin        signal peptide fused to a tissue-type plasminogen activator        propeptide (IgSP-tPA), murine immunoglobulin signal peptide        (IgSP), a MPIF-1 signal sequence (MKVSVAALSCLMLVTALGSQA (SEQ ID        NO: 15)); a stanniocalcin signal sequence (MLQNSAVLLLLVISASA        (SEQ ID NO:16)); an invertase signal sequence        (MLLQAFLFLLAGFAAKISA (SEQ ID NO:17)); a yeast mating factor        alpha signal sequence (K. lactis killer toxin leader sequence);        a hybrid signal sequence (MKWVSFISLLFLFSSAYSRSLEKR, (SEQ ID        NO:18)); a HSA/MFα-1 hybrid signal sequence        (MKWVSFISLLFLFSSAYSRSLDKR (SEQ ID NO:19)); a K. lactis        killer/MFα-1 fusion leader sequence (MNIFYIFLFLLSFVQGSLDKR (SEQ        ID NO:20)); an immunoglobulin Ig signal sequence        (MGWSCIILFLVATATGVHS (SEQ ID NO:21)); a Fibulin B precursor        signal sequence (MERAAPSRRVPLPLLLLGGLALLAAGVDA (SEQ ID NO:22));        a clusterin precursor signal sequence (MMKTLLLFVGLLLTWESGQVLG        (SEQ ID NO: 23)); and the insulin-like growth factor-binding        protein 4 signal sequence (MLPLCLVAALLLAAGPGPSLG (SEQ ID NO:24))        or a functional fragment thereof    -   18. The method of paragraph 1, wherein the recombinant MIS        protein comprises a modification of amino acid 450 of SEQ ID NO:        1 from Q to R to increase cleavage as compared to in the absence        of such a modification.    -   19. The method of paragraph 1, wherein the recombinant MIS        protein comprises a modification of amino acid 452 of SEQ ID NO:        1 from S to R to increase cleavage as compared to in the absence        of such a modification.    -   20. The method of paragraph 9, wherein the tag is a FLAG tag        comprising amino acid sequence of SEQ ID NO: 8 or a functional        fragment thereof    -   21. The method of paragraph 20, wherein the FLAG tag is located        after amino acid residue 452 of SEQ ID NO: 1 and before amino        acid residue 453 of SEQ ID NO: 1.    -   22. The method of paragraph 20, wherein the FLAG tag is located        between amino acid residue 452 and 453 of SEQ ID NO: 1.    -   23. The method of paragraph 1, wherein the recombinant MIS        protein comprises the amino acid residues 25-559 of SEQ ID NO: 2        or a functional fragment thereof.    -   24. The method of paragraph 1, wherein the recombinant MIS        protein comprises the amino acid residues 25-567 of SEQ ID NO: 3        or a functional fragment thereof.    -   25. The method of paragraph 1, wherein the recombinant MIS        protein is encoded by a vector.    -   26. The method of paragraph 25, wherein the vector is a viral        vector or an expression vector.    -   27. The method of paragraph 26, wherein the expression vector is        pcDNA 3.1 or cDNA or genome vector for bacteria (e.g., e coli)        or bacteriophage.    -   28. The method of paragraph 26, wherein the viral vector is        selected from the group consisting of an adenoviral vector, a        poxvirus vector and a lentiviral vector.    -   29. The method of paragraph 26, wherein the viral vector is an        adeno-associated vector (AAV).    -   30. The method of paragraph 29, wherein the adeno-associated        vector (AAV) is AAV9.    -   31. The method of any of paragraphs 25 to 30, wherein the vector        encodes a recombinant MIS protein or fragment thereof which has        at least 95% sequence identity to the nucleic acid sequence of        SEQ ID NO: 4 or SEQ ID NO: 5, wherein the nucleic acid sequence        is operatively linked to a tissue- or cell-type specific        promoter.    -   32. The method of any of paragraphs 25 to 31, wherein the vector        expresses the recombinant MIS protein at a constant level over a        period of time.    -   33. The method of paragraph 1, wherein administering is        intravenous, intrapsinal, intradermal, intramuscular,        intraarterial, intralesional, percutaneous, or subcutaneous, or        by aerosol.    -   34. The method of paragraph 1, wherein administering is        prophylactic administration.    -   35. The method of paragraph 1, wherein administering is        therapeutic administration.    -   36. The method of paragraph 1, wherein the subject is a mammal.    -   37. The method of paragraph 36, wherein the mammal is a human.    -   38. The method of paragraph 1, wherein at least one additional        agent is administered to the subject in combination with (e.g.,        before, during or after) administration of the recombinant human        MIS.    -   39. The method of paragraph 38, wherein the additional agent is        a therapeutic agent or neurotrophic factor selected from the        group consisting of; riluzole, glial cell line-derived        neurotrophic factor (GDNF), brain derived neurotrophic factor        (BDNF), ciliary derived neurotrophic factor (CNTF), glutamate,        and gonadal hormone.    -   40. Use of recombinant MIS protein for the manufacture of a        medicament for a neurodegenerative disease or disorder, wherein        the recombinant MIS protein comprises a modification of amino        acid 450 of SEQ ID NO: 1 from Q to R.    -   41. The use of paragraph 40, wherein the recombinant MIS protein        further comprises a Tag protein.    -   42. The use of paragraph 41, wherein the tag is a FLAG tag        comprising amino acid sequence of SEQ ID NO: 8 or a functional        fragment thereof    -   43. The use of paragraph 40, wherein the recombinant MIS protein        is encoded by a vector.    -   44. The use of paragraph 40, wherein the MIS receptor is MIS        type II receptor or a homologue or functional fragment thereof.

The invention can be understood more fully by reference to the followingdetailed description and illustrative examples, that are intended toexemplify non-limiting embodiments of the invention.

EXAMPLES

The following examples are provided for illustrative purposes only andare not intended to limit the scope of the invention.

The description of the present invention has been presented for purposesof illustration and description, but is not intended to be exhaustive orlimiting of the invention to the form disclosed. The scope of thepresent invention is limited only by the scope of the following claims.Many modifications and variations will be apparent to those of ordinaryskill in the art. The embodiment described and shown in the Figs. waschosen and described in order to best explain the principles of theinvention, the practical application, and to enable others of ordinaryskill in the art to understand the invention for various embodimentswith various modifications as are suited to the particular usecontemplated.

Materials and Methods

Constructs and Plasmid Cloning.

WT-MIS: pBG311 Vector with Genomic Sequence of MIS.

The vector was constructed as previously described (Cate et al, 1986).Briefly, the genomic sequence of human MIS was sub-cloned into a pBG311expression vector from chMIS33 which was isolated from a human cosmidlibrary using a bovine cDNA probe (Cate et al. 1986).

RF-MIS: pcDNA 3.1 and pAAV-IRES-NEO Vectors Containing MIS cDNA withNative MIS Leader Sequence, Modified Cleavage Site (R), and Flag Tag(F).

The coding sequence of MIS, present in a pcDNA3.1 vector containing aFLAG-labeled full-length human MIS cDNA sequence previously described(Papakostas et al, 2010) was subcloned into a pAAV-IRES-Neo expressionvector at an ECORV site. This coding sequence contains a FLAG-epitopeinserted after a modified cleavage site at position 428 (RARR/S) (SEQ IDNO: 27) FLAG (Papakostas et al, 2010).

LR-MIS: pcDNA 3.1 Vector Containing MIS cDNA with Human Serum AlbuminLeader Sequence (L) and Modified Cleavage Site (R).

The pcDNA3.1 vector containing a full-length human MIS cDNA sequencecontaining a modified cleavage site, as previously described (Papakostaset al, 2010) was used to incorporate the albumin leader sequence. Thealbumin leader was cloned in the place of the MIS leader using a forwardprimer containing an EcoRV site:CGAGATACATGAAGTGGGTGAGCTTCATCAGCCTGCTGTTCCTGTTCAGCAGCGCTTACTCCCGCGGTGTGTTCCGGCGCAGAGCAGAGGAGCCAGCTGTG (SEQ ID NO: 11) (with thenucleic acid encoding the leader sequence highlighted in bold) and abackward primer at position 451-432 of MIS GCTCCTGGAACCTCAGCGAG (SEQ IDNO: 12).

LRF-MIS: pcDNA 3.1 Vector Containing MIS cDNA with Human Serum AlbuminLeader Sequence (L), Modified Cleavage Site (R) and Flag Tag (F).

The pcDNA3.1 vector containing a full-length human MIS cDNA sequencecontaining a modified cleavage site and a flag tag, as previouslydescribed (Papakostas et al, 2010) was used to incorporate the albuminleader sequence as described above.

Transfections and Cloning:

Wild-type MIS (WT-MIS). The WT-MIS construct (pBG311) along withpSV2DHFR was previously transfected in DHFR-CHO cells and the B9 clonewas selected as the highest expresser as previously described (Cate etal, 1986).

RARR/S-Flag MIS (RF-MIS) (“RARR/S” Disclosed as SEQ ID NO: 27):

The RF-MIS construct (in pAAV-IRES-NEO) was transfected in CHO-S cellsusing Fugene 6 (Roche) according to the manufacturer's protocol and theCHO93 stably expressing clone was selected under geneticin selection(550 ug/ml) as the highest expresser determined by western blot.

LR-MIS.

The LR-MIS construct (in pcDNA3.1) was transfected in CHO-K1 cells usinglipofectamine 2000 (invitrogen), according to the manufacturer'sprotocol. Clones were selected in 800 ug/ml of geneticin, and thehighest expressers as determined by western blot (LR8, 11 and 22) werechosen for further study.

LRF-MIS.

The LRF-MIS construct (in pcDNA3.1) was transfected in CHO-K1 cellsusing lipofectamine 2000 (invitrogen), according to the manufacturer'sprotocol. Clones were selected in 800 ug/ml of geneticin (G418), and thehighest expressers as determined by western blot (LRF8, 18 and 22) werechosen for further study.

Media and Culture Conditions:

WT-MIS; B9 Clone.

B9 is grown in roller bottles (1700 cm²) with 250 ml of alphaMEM-supplemented with 5% female fetal calf serum (FFCS) (Biologos), 0.24μM methotrexate, 2 nM glutamine, 100 U/ml penicillin and 100 ug/mlstreptomycin (Invitrogen) maintained confluent for several months in 5%CO2, at 37 C while media is collected every 3-4 days. Media is screenedby western and MIS ELISA to monitor and measure production.

RF-MIS: CHO93 clone.

CHO93 is grown in roller bottles (1700 cm²) with 250 ml of DMEM:F12supplemented with 10% FFCS, 550 ug/ml of geneticin, 2 nM glutamine, 100U/ml penicillin and 100 ug/ml streptomycin (Invitrogen) maintainedconfluent for several months in 5% CO2, at 37 C while media is collectedevery 3-4 days. Media is screened by western and MIS ELISA to monitorand measure production.

LR-MIS and LRF-MIS: LR8, 11, 22 and LRF8, 18, 22 Clones.

Both LR-MIS and LRF-MIS clones are grown in roller bottles (1700 cm²)with 250 ml of DMEM supplemented with 10% FFCS, 800 ug/ml of geneticin,2 nM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin(Invitrogen) maintained confluent for several months in 5% CO2, at 37 Cwhile media is collected every 7 days. Media is screened by western andMIS ELISA to monitor and measure production.

Purification of MIS.

Purification Using Immunoaffinity Anti-Flag Beads.

RF-MIS and LRF-MIS, which contain a flag tag, are isolated fromserum-containing media collected from roller bottles of stablyexpressing clones of CHO (CHO93, LRF8, LRF18, LRF22) as described above.Collected media is spun down to discard dead cells and the supernatantis collected into 500 ml containers and stored in −20 C untilpurification. For purification, media is thawed at 4 C overnight andthen incubated with anti-FLAG agarose beads (SIGMA, 500 μl packedbeads/500 ml media), mixing with rotation overnight at 4 C.Subsequently, the beads are precipitated at 13000 rpm, for 10 secondsand washed extensively (7×) with cold 1× Tris Buffered Saline (TBS)(SIGMA). All reagents are kept on ice. RF-MIS and LRF-MIS is eluted with50 μg of 3× FLAG peptide (SIGMA)/500 μl beads in 1×TBS at 25 C for 45minutes with rotation. The beads are spun down at 13000 rpm, for 10seconds at room temperature and the supernatant containing the FLAG MISis collected, aliquoted, and stored in low protein binding Eppendorftubes (VWR) at −80 C for subsequent use.

Immunoaffinity Purification Using Anti-MIS 6E11 Immunoaffinity Column.

The 6E11 MIS monoclonal antibody column was produced as previouslydescribed (Ragin et al, 1992). Briefly, a 5 ml immunoaffinity column wasconstructed using approximately 50 mg of protein A—sepharose (SigmaChemical Co., St Louis, Mo.)—purified mouse monoclonal anti-human rhMISantibody, as previously described. [Ragin 1992, Hudson 1990], covalentlyattached to 5 ml packed Affigel-10 agarose resin (Biorad Laboratories,Richmont, Calif.) per manufacturer's instructions (approximately 80%coupling efficiency). The column was blocked with ethonalamine andequilibrated with 50 ml of 20 mM4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Hepes), pH 7.4 and200 ml concentrated (10×, serum free) conditioned media loaded at 1column vol/h at 4° C. After loading, the column was washed with 10column volumes of 20 mM Hepes, pH 7.4. A pre-elution step employed 1column volume containing 0.5M NaCl in 20 mM Hepes, pH 7.4. Elution ofbound rhMIS was achieved using 1M Acetic Acid in 20 mM Hepes, pH 3.0.The majority of the rhMIS eluted in a 2-5 ml fraction, post 2 ml voidvolume fraction. Eluted rhMIS was immediately neutralized with NaOH to apH between 7.0 and 7.4. The acid eluted fractions were dialyzedovernight versus 0.02M Hepes, pH7.4. The resulting rhMIS was analyzedfor total protein by Bradford method (Bradford, 1976) and for rhMISconcentrations by an enzyme-linked immunoassay (Hudson 1990) andexamined by polyacrylamide gel electrophoresis (Weber 1969), Westernblot analysis (Towbin 1979), in vitro Mullerian duct regressionbioassays and tumor antiproliferative assays (Chin 1991).

ELISA

ELISAs were performed using the antibody “sandwich” method using platescoated with anti-holo MIS 6E11 mouse monoclonal antibody (whichrecognizes cleaved and uncleaved products) and the MGH6 rabbitpolyclonal anti holo-MIS (which recognizes cleaved and uncleavedproducts) as reported previously 5. In detail, Dynatech Immulon 2HBElisa 96-well flat bottom plates (Thermoscientific, Rochester, N.Y.)were coated overnight at 4 C with mouse monoclonal 6E11 anti-hrMISantibody (described above) in 0.05M sodium bicarbonate buffer pH 9.6 (5μg/ml; 50 μl/well). The plates were washed five times with washingbuffer (phosphate buffered saline (PBS)/0.1% Tween 20) (150 μl/well) andblocked with 1% BSA in PBS (IgG-free, protease-free) (JacksonImmunoResearch Laboratories, West Grove, Pa.) for 2 hours at roomtemperature or overnight at 4 C. This blocking buffer was used for allsubsequent dilutions wherever mentioned. Plates were sealed to preventdehydration. The blocking buffer was discarded and the plates werewashed with washing buffer. The concentration of the MIS standards weredetermined by Bradford. MIS standards or unknowns were diluted inblocking buffer and were incubated overnight at 4 C. All sampleincubations were done at a volume of 500 per well. After five washeswith washing buffer, the rabbit polyclonal anti MIS antibody (MGH6;developed in our laboratories) was added at 1:4000 dilution in blockingbuffer and was incubated for 1 hour at room temperature. The plates werewashed five times with washing buffer. Donkey anti rabbit IgG conjugatedto HRP (Jackson ImmunoResearch Laboratories, West Grove, Pa.) was addedat 1:70000 dilution in PBS, and the plates were incubated for 1 hour at4 C. After five washes with washing buffer, 500 of 0.42 mM TMB in 0.1Msodium acetate citric acid (citrate buffer) pH 4.9/0.044% H2O2 was addedto each well and the reaction was monitored 12.5 minutes at roomtemperature in the dark. The reactions were quenched by the addition of2N sulfuric acid, and absorbances were read at 595 nm on a microplatereader (Victor2 1420, Perkin Elmer Lifesciences, Shelton, Conn.). TheELISA of the LR and LRF clones was performed on the media of confluentplates incubated for 24 hours, after which the number of cells wascounted to estimate the production (pg/cell/day) which was displayed asthe mean of 5 experiments.

Electrophoresis and Western Blotting

Samples for gel electrophoresis were reduced with 100 mM Dithiothreitolin 1× Laemmli buffer (0.0625 mM Tris pH 6.8, 2% (w/v) SDS stock, 10%(v/v) glycerol, 0.002% (w/v) bromophenol blue) and heat denatured on athermoblock at 70° C. for 10 min. Samples were run on a 4-12% Tris-BisNuPage Novex “mini” gel (Invitrogen) at 130V with 1×(N-morpholino)ethanesulfonic acid (MES) running buffer (Invitrogen).Gels were stained with LUMITEIN™ (Biotium) or coomassie stain (0.3%Brilliant Blue R-250, 45% Methanol, 10% Acetic Acid in H₂O) for 15 minat room temperature with agitation. Subsequently, they were agitatedovernight at room temperature in de-staining solution (20% methanol, 10%acetic acid in H₂O) with agitation.

For Western blot analysis, gels were transferred onto PVDF (Millipore)membranes, previously equilibrated in 1× NuPage transfer buffer(Invitrogen) containing 12% (v/v) methanol, at 25V for 45 min and at 35Vfor another 45 min. Membranes were blocked with 1×PBS, 0.1% Tween-20containing 5% nonfat dry milk for 30 min at room temperature and probedwith horseradish peroxidase conjugated mouse monoclonal anti-FLAG M2antibody (SIGMA) (1:1000), goat C20 anti-MIS c-terminus antibody (SantaCruz) (1:200) or rabbit MGH4 anti-MIS n-terminus MIS antibody (custom)(1:1000). Blots were washed two times 5 min each at room temperaturewith 1×PBS, Tween-20 0.1%, and incubated with appropriate secondaryantibody if necessary, and washed three times (5 mins). Proteins bandswere visualized with the ECL kit detection system (Perkin-Elmer) ontoKodak Biomax MR film. ImageJ (NIH, see world wide web at:“imagej.nih.gov/ij/”) was used to perform densitometry to quantify theprotein bands to compare cleavage of different constructs which wasaveraged over at least three independent western blots.

Animals and Organ Cultures:

The standard organ culture bioassay for Mullerian Inhibiting Substance(MIS) was performed as described previously (Donahoe, 1977). Briefly,female urogenital ridges from timed pregnant rats at E14.5 (Harlan) weredissected and cultured on agar coated stainless steel grids mountedabove fortified Cambridge Medical Research Laboratories (CMRL) 1066media (Life Technologies) supplemented with 10% FFCS (to avoid an effectof bovine MIS in male serum), 1% penicillin/streptomycin, 1%L-Glutamine, 1% Fungizone (Invitrogen), and 1 nM testosterone (Sigma).After incubation for 72 hours in humidified 5% CO2 at 37° C., thespecimens were fixed in Zamboni buffer (15% formaldehyde solution, and5% picric acid), and embedded in paraffin, and 8-um sections of thecephalic end were stained with hematoxylin and eosin. The sections werethen scored from 0 (no regression) to 5 (complete regression), by twoexperienced observers. Cultures were carried out with conditioned media(mock) and with replicates (N of at least 3) of purified RF-MIS,LRF-MIS, LR-MIS, or WT-MIS at a final concentration of 5 μg/ml and atlower doses of 3 μg/ml, and 1 μg/ml. LR-MIS was also tested atconcentrations of 0.5 μg/ml and 0.2 μg/ml.

Administration of rAAV9-mMIS In Vivo

rAAV9-mMIS (mouse) was prepared using methods as disclosed herein andcommonly known to persons of ordinary skill in the art and rAAV9-mMISadministered at day 1 after birth (P1) using the facial vein. The doseis scaled by weight of the recipient mice to 4×10¹⁴ rAAV9-mMISgenomes/kg, at a concentration of 4×10⁹/μl. Thus, for a newborn mousethe typical dose is 4×10¹¹ genomes in 100 μl. Control mice were injectedwith empty rAAV9 at the same dose and concentration. The recipientSOD1G93A mutant mice can be obtained from Jackson Labs. The inventorselected to use the congenic SOD1G93A all B6 strain for two reasons.First, mice within this congenic strain should be genotypicallyidentical; avoiding some of the inter-individual variability inherent inusing the more frequently studied hybrid B6SJL SOD1G93A ALS mice. And,second, the ALS trait in the congenic B6 strain is associated with aslightly longer survival (˜160 days) as compared to the SJL-B6 strain(˜135 days). This slightly milder phenotype enhances the likelihood ofdetecting benefit from AAV9-mMIS intervention.

Analysis of ALS disease phenotype in ALS mice

All mice were evaluated twice weekly from 50 days of age onward; in eachof these initial evaluations the weight and results of the leg extensiontest are tested. Briefly, at each evaluation a neurological score isassigned on a scale of 0 to 4 (Jackson Laboratory Manual on ALS Mice),for which 0 is normal; 1 corresponds to limb trembling or partialcollapse of the outstretched limbs toward the midline; 2 representsdragging of a limb; 3 is paralysis or foot dragging; and 4 is theend-stage at which the animal cannot right itself in 30 seconds afterbeing placed on its side. The onset of the motor neuron disease isdefined as the time point at which the mice either show a score ofneuroscore of 1 two days in a row or develop weight loss of 5% from anapparent peak weight. After onset, the mice were examined every otherday, still obtaining weights and a neuroscore. Motor function isrecorded using grip strength and rotorod on a weekly basis. Mice werefollowed until the neuroscore is 4, at which point they are euthanized.At death, and for cohorts of control and treated mice at two specifictimes point mid-way in the disease (110 and 130 days for the B6 ALSmice), a series of quantitative histological studies are performed,which include western blot of MIS expression in vivo from the i.v.injection of AAV9-MIS, analysis of neuromuscular junction architecture,electromyography testing to assess motor unit size (MUS) and motor unitnumber estimates (MUNE), and ventral root counts, as well as copy numberand expression analysis of SOD1. All mouse studies were performed in ablinded manner by an experienced mouse technician.

Example 1

Purification of Mullerian Inhibiting Substance (MIS) protein forpreclinical efficacy (Pieretti-Vanmarcke et al. 2006), has predominantlybeen done from conditioned media from CHO cells transfected with agenomic clone (Cate et al. 1986). The media was then immunoaffinitypurified (Ragin et al. 1992) using a mouse monoclonal antibody (Hudsonet al. 1990) or purified by serial chromatography (Lorenzo et al. 2002).Biologic activity was detected in an embryonic organ culture Mullerianduct regression assay (Donahoe et al. 1977) and immunoactivity detectedby an ELISA (Hudson et al. 1990) using monoclonal and polyclonalantibodies raised to human MIS. The transfected CHO cells weresubsequently adapted to serum free conditions and suspension culture(MacLaughlin/Stafford/Dean, Donahoe unpublished), clonally selected,scaled, and purified as above. Western analysis confirmed 25-30%cleavage to yield the homodimerized C-terminus bioactive moeity whichwas held in noncovalent association with the homodimerized N terminus,with cleavage at the Kex-like, primary cleavage site at amino acidresidues 426-427, and secondary cleavage at amino acid positions229-230. Bands on reduced electrophoretic gels at 70, 55, 34, 24, and12.5 kDa were all MIS fragments, as determined by amino acid sequencing(Ragin et al. 1992; Lorenzo et al. 2002), and representative ofpredicted Kex and dibasic cleavage products.

To optimize cleavage and the primary cleavage site at amino acidposition 428, the recognition sequence was mutagenized to create adibasic cleavage site; the RAQR/R (SEQ ID NO: 28) variant was bioactive(Kurian et al, 1994). Position 425 (corresponding to amino acid residue450 of SEQ ID NO: 1) was then mutagenized to create a more consensus Kexcleavage site (Nachtigal & Ingraham 1996)(Hosaka et al. 1991), RARR/S(SEQ ID NO: 27), and an 8 amino acid Flag (DYKDDDDK) (SEQ ID NO:8) tagwas added just downstream of the first serine in the C-terminus to aidin detection and purification. Expression of this variant resulted inimproved cleavage and increased bioactivity. By comparison, when theC-terminal arginine (Kurian et al 1994) was followed by Flag, theprotein produced by this construct was bioinactive (Papakostas et al2010); thus, the serine appeared to be important for preservation ofbioactivity. The RARR/S (SEQ ID NO: 27) Flag construct (Papakostas et al2010) was transfected into CHO cells and improved cleavage andpreservation of bioactivity confirmed (Papakostas et al, 2010). Themodification of the cleavage site increased the cleavage to over 50-60%(Papakostas et al, 2010).

To scale expression, the MIS RARR/S (SEQ ID NO: 27) Flag construct wasfurther modified to substitute the endogenous MIS leader sequence withthat of human serum albumin (HSA). HSA is the most abundant protein inplasma and is produced at a very high rate by the liver to achieve ablood concentration ranging from 3.4 to 5.4 g/dL (Farrugia 2010). Theproduction and processing of HSA is finely tuned to allow efficientmaturation and secretion of the protein. HSA, like MIS is synthesized asa prepro-protein, which contains a leader sequence that is subsequentlycleaved during maturation. This HSA leader sequence consists of only 24AA, is not immunogenic in humans, and is removed during proteinprocessing. Here the inventors demonstrate that substitution of the MISleader sequence with that of HSA increases production, and unexpectedly,cleavage, which correlates with increased potency of the recombinanthuman MIS product.

Example 2

Design of Novel Recombinant MIS Constructs, and Isolation of CHO Clones.

The inventors have developed new constructs using the human cDNAsequence of MIS to improve upon the original wild type (WT) genomic MISconstructs in an effort to increase production of recombinant MIS with asequence size more amenable to other applications such as viral genetherapy. Three modifications were evaluated herein: a Q425R amino acidsubstitution in the c-terminal maturation cleavage site annotated as“R”, the addition of a FLAG-tag on the N-terminus of the c-terminalmature peptide at amino acid (AA) position 428 annotated as “F”, and asubstitution of the endogenous MIS leader peptide with the human serumalbumin leader (HSAL) peptide directly upstream of AA position 0annotated as “L” (Table 2 and FIG. 1A). The resulting constructs whichincorporate these modifications are thus referred to as RF-MIS (566AA),LRF-MIS (567AA) and LR-MIS (559AA) (FIG. 1B). The 1AA difference betweenRF-MIS and LRF-MIS results from the fact that the HSAL peptide is IAAlonger than that of MIS, which otherwise shares 20% identity (FIG. 1A).Both the WT genomic MIS, referred to as “WT-MIS”, and the RF-MISconstructs have been previously described (Papakostas et al, 2010). Thenovel LR-MIS and LRF-MIS transgenes were cloned in a pcDNA3.1 mammalianexpression vector, and stably transfected into CHO-K1 cells (Table 3).The three highest expressing clones for the LR-MIS construct (LR8, LR11,LR18), and the LRF-MIS construct (LRF8, LRF18, LRF22) were selected fromhundreds of screened clones by comparing MIS level in the media of eachclone by western blot (data not shown).

By substituting the modified RARR/S (SEQ ID NO: 27) for the endogenousRAQR/S (SEQ ID NO: 26) (noted as R in constructs), and inserting a Flagtag immediately downstream of the cleavage site (noted as F inconstruct) (Table 2). (FIG. 1B), the inventors demonstrated increasedcleavage of the tagged C-terminus (Papakostas et al, 2010). Furthermore,the recombinant RARR/S-Flag MIS (“RARR/S” disclosed as SEQ ID NO: 27)(referred to herein as “RF-MIS”) protein retained bioactivity in thefetal rat urogenital ridge assay (Papakostas et al, 2010). To overcomelow expression yields, the backbone vector of RF-MIS was switched topAAV-IRES-Neo, and cloned into CHO-S cells, and screened under highGeneticin concentration. The resulting expression vector ispolycistronic and includes an internal ribosomal entry site (IRES)driving expression of the neomycin resistance cassette downstream ofMIS, allowing for better selection of high expressers. The highestexpressing clone, CHO93, was subsequently scaled up for production usingroller bottles and recombinant RF-MIS was purified using anti-flag M2immunoaffinity beads (Table 3). However, while RF-MIS has increasedcleavage of the active C-terminus, and importantly, less internalcryptic cleavage (FIG. 2) (FIG. 3), the yield and production of the cDNAclone CHO93 (0.16 pg/cell/day) remains much lower than that of thegenomic clone B9 (10.59 pg/cell/day)(Table 4A), although it is unclearwhether this is due to the expression vector, the CHO cells, the natureof the drug selection, or the type of message produced (cDNA versusgenomic MIS).

To improve production, the original R-MIS and RF-MIS construct inpcDNA3.1 vectors were modified by substituting the 25AA MIS leadersequence (pre-pro peptide) with the 24 AA of the human serum albumin(HSA) leader sequence (herein noted as L in constructs) to create the“LR” and “LRF” constructs (Table 2) (FIG. 1A).

TABLE 2List of modifications to the MIS wild-type sequence and corresponding nomenclature.Position (AA) Position on Modification (shown in (normal protein SEQ IDNotation Native BOLD) nomenclature) NO: 1 Purpose R RAQR/SRARR/S (SEQ ID NO: 27) 423-427 448-452 Furin/Kex2 census (SEQ IDsite for improved NO: 26) cleavage F n/a FLAG Tag Located LocatedC-terminus FLAG (DYKDDDDK) (SEQ ID between 427- between tag for easierNO: 8) 428 452-453 of purification and SEQ ID tracking. NO: 1 L MISHuman serum albumin 1-25 1-25 Increased Leader (HSA) Leader Sequenceproduction, secretion Sequence (MKWVTFISLLFLFSSA and cleavage.YSRGVFRR) (SEQ ID NO: 6)

HSA leader sequence fusion has been shown to increase production ofrecombinant interleukins (Carter et al, 2010) and TNF-alpha (Maeda Y etal 1997), and has been suggested as a way to produce proteins otherwisedifficult to express and to scale. Furthermore, HSA is known to alsoenhance secretion of fused proteins such as human lysozyme in yeastexpression system with Pichia pastoris (Xiong et al, 2008). The threehighest stably expressing clones in CHOK1 were selected for furtheranalysis: LR8/11/18 and LRF8/18/22 (FIG. 2). Both cloning efficiency andexpression levels were greater for the LR clones than the LRF clones,demonstrating that the FLAG tag may make expression less efficient.Similarly to CHO93, all LR and LRF clones have reduced peptide fragmentsresulting from internal cryptic cleavage at position 229, when comparedto the wild type (WT-MIS) protein produced by B9. Unexpectedly, theyalso appear to have greater proportion of cleaved C-terminus (FIGS. 2and 3). This increased cleavage could be explained by the strongevolutionary pressures on the albumin leader for efficient processing inthe trans-golgi network and transport to secretory vesicles, sincealbumin is endogenously secreted at much higher rate than MIS(Rothschild et al. 1988). LRF18 was chosen for characterization since itis the highest expressing LRF clone, and can be purified and trackedusing the Flag tag (Table 3).

TABLE 3 List of constructs and cell line clones producing MIS andcorresponding purification methods. Construct Clones Vector Cell LinePurification MIS B9 MIS WT genomic CHO cells lacking Immunoaffinityusing sequence in pBG311 the DHFR gene. 6E11 monoclonal plasmid.antibody against MIS or serial chromatography. RF-MIS CHO93 MIS cDNAsequence CHO-S Immunoaffinity using M2 inserted into pAAV- monoclonalantibody IRES-Neo plasmid. against FLAG tag. LR-MIS LR8 MIS cDNAsequence CHO-K1 Immunoaffinity using LR11 inserted into 6E11 monoclonalLR18 pcDNA3.1plasmid. antibody against MIS or serial chromatography.LRF-MIS LRF8 MIS cDNA sequence CHO-K1 Immunoaffinity using M2 LRF18inserted into monoclonal antibody LRF22 pcDNA3.1plasmid. against FLAGtag.

When cultured for 24 hours in flasks, the concentration of MIS, asdetected by ELISA, is greater in the media of B9 (WT-MIS) (15 μg/ml)than in the media of clones (LR8: 3.04 μg/ml; LR11: 11.66 μg/ml; LR22:6.28 μg/ml) (Table 3). The highest producing clone of LR, LR11 secretes3.24 pg/cell/day of MIS while the WT clone B9 produces 10.58pg/cell/day, however, LR11 cells grow much more compact fashion,conversely, the highest expressing clone of LRF, LRF18 has both higherconcentration (1.1 μg/ml) and higher production (0.26 pg/cell/day) thanRF-MIS (CHO93) with (0.67 μg/ml) and (0.15 pg/cell/day) (Table 4A).

The data for expression of clones WT-MIS (B9), RF-MIS (CHO93), and thehighest expressing clones for LR-MIS (LR11) and LFR-MIS (LRF18) inserum-containing and serum-free media is summarized in Table 4B. Whencorrected for the number of cells, the highest producing clone of LR-MIS(LR11), secretes 1.142+/−0.482 pg/cell/day of MIS, significantly less(p=0.01) than the WT-MIS clone (B9) with 7.597+/−1.378 pg/cell/day inserum containing media. Furthermore, the highest expressing clone ofLRF, LRF18, has both a significantly higher concentration, 2.149+/−0.479μg/ml (p=0.03), and a significantly higher production, 0.430+/−0.177pg/cell/day (p=0.04), than the RF clone CHO93 with 1.236+/−0.772 μg/mland 1.236+/−0.772 pg/cell/day respectively in serum containing media.These trends of expression as detected by ELISA are recapitulated inlarger culture vessels (200 ml roller bottles), and are consistent withthe amount of c-terminal MIS observed in the media by western blot (FIG.2), with the highest MIS concentrations achieved in roller bottlesattaining up to 20 μg/ml for B9, 25 μg/ml for LR11, 4 μg/ml for LRF18,and 2 μg/ml for CHO93.

Thus, the addition of the HSA leader increases the production of theflag-tagged MIS product but not the untagged product. However, as theflag-tagged constructs clearly do not produce as much as the untaggedones, the flag tag may be interfering with protein stability orexpression. Coomassie stains and western blot show that the productpurified from LRF18 by anti-flag immunoaffinity purification has fewerbands representative of internal cleavage (Ragin 1992) than the MISpurified from WT-MIS (B9) using anti-MIS affinity purification (FIG. 3).

TABLE 4A Purification yield from MIS from various constructs. WT-MISRF-MIS LRF-MIS (B9) (CHO93) (LFR18) LR-MIS (LR11) MIS 15 0.67 1.10 11.67concentration (μg/ml) at 24 hours Production 10.59 0.15 0.26 3.24(pg/cell/day) Purification yield 15% 20% 20% 15% (% w/w) Percentcleavage 20% 50% 90% 90%

TABLE 4B yield of expression of clones WT-MIS (B9), RF-MIS (CHO93), andthe highest expressing clones for LR-MIS (LR11) and LFR-MIS (LRF18) inserum-containing and serum-free media. RF-MIS LRF-MIS LR-MIS WT-MIS (B9)(CHO93) (LFR18) (LR11) MIS concentration (μg/ml) 16.821 ± 3.393  1.236 ±0.772 2.149 ± 0.479 4.866 ± 1.238 at 24 h in serum-containing mediaProduction of MIS 7.597 ± 1.378 0.254 ± 0.184 0.430 ± 0.177 1.142 ±0.482 (pg/cell/day) in serum- containing media MIS concentration (μg/ml)1.528 ± 0.105 0.223 ± 0.063 0.457 ± 0.254 1.411 ± 0.249 at 24 h inserum-free media Percent cleavage at 24 h in 25 ± 5  50 ± 19 37 ± 28 79± 5  serum-free media (%) Purification Yield (% w/w) 15 20 20 15

Taken together, the inventors demonstrate herein that the LR-MIS resultsin a greater yield of production with increased cleavage and higherbioactivity or potency. Accordingly, the inventors demonstrate that theHSA leader sequence surprisingly resulted in an increased yield (bothhigher concentration and higher production) of the recombinant human MISprotein (see FIGS. 2 and 3).

Comparison of Cleavage in MIS-Producing Clones.

To estimate the amount of cleavage of the C-terminal MIS, MIS-producingclones were grown in serum-free media since the albumin in the seruminterferes with the detection of holo-MIS by western blot (FIG. 2B).When examining the ratio of bands using a C-terminus antibody bydensitometry analysis of Western blot of conditioned serum free media,the LR-MIS clone LR11 displays over 79% cleavage, while WT-MIS producedby clone B9 shows only 25%. Comparatively, the Flag-containing clonesLRF18 and CHO93 have 37% and 50% cleavage respectively (Table 4B). Thecleavage was calculated using at least 4 independent experiments bywestern blot and indicates that the cleavage of LR11 is significantlyhigher than that of B9 or LRF18 (p<0.01). The cleavage of MIS in themedia translates into an increase in cleaved c-terminal MIS, and acomplete absence of unwanted internal cryptic cleavage product in theimmunoaffinity purified recombinant LR-MIS and LRF-MIS as observed bywestern blot analysis of 0.1 μg of purified material, or in gelelectrophoresis with non-specific protein staining using 1 μg ofpurified material (FIG. 3B).

Accordingly, the presence of the HSA leader sequence also resulted in anunexpectedly increase in cleavage from the primary cleavage site(corresponding to cleavage at 451/452 of SEQ ID NO: 1 (or 426/427 ofconventional amino acid nomenclature of wild-type human MIS protein)(see FIGS. 2A-2B and 3B). This increased yield and increased cleavagewas surprising because with an increased yield (and therefore moreprotein produced by the cell), one would expect a decreased cleavage asthe activity of the available cleavage enzymes becomes saturated andoverextended. However, this was not the case—in fact the exact oppositeoccurred where with increased protein production there was increasedcleavage from the primary cleavage site.

This is particularly unexpected as the effect of the leader sequence,which is not located anywhere near the cleavage site of the primarycleavage site of MIS, was not expected to have an effect on increasedcleavage as the leader sequence is typically cleaved first before thepost-translation cleavage of the proprotein MIS.

Furthermore, the leader sequence also resulted in less cleavage from thesecondary cleavage site (located between amino acid residues 229/230 ofnormal wild-type MIS numbering or corresponding to residues 254/255 ofSEQ ID NO: 1). This is also surprising, considering that there was not amodification to the secondary cleavage site.

Additionally, the presence of the leader sequence also increased theproduction and yield even when a FLAG tag is present in the recombinanthuman MIS protein. (The FLAG tag significantly decreases the yield asshown in Table 4A and 4B). This again was a surprising discovery, as theleader sequence is not located anywhere near the FLAG tag and it wouldnot be expected that such a modification to the leader sequence wouldincrease the production yield of a protein comprising a FLAG tag.

Bioactivity of Purified MIS.

Since the C-terminus of MIS has previously been shown to be the activemoiety (Pepinski 1988, Maclaughlin et al 1992), increased cleavageshould correlate with greater bioactivity in the rat UGR assay. Toverify that the modifications to the protein sequence do not interferewith the activity of MIS, purified MIS was tested in an ex-vivourogenital ridge culture assay. The ridges, dissected from female ratfetuses of E14.5 of age, contain gonadal tissue, Mullerian and Wolffianducts, and much smaller mesonephric ducts. Ridges are incubated 72 h atthe air/media interface on grids containing an agarose substrate; themedia is supplemented with 1, 3, or 5 μg/ml of MIS (FIG. 4A), or down to0.5 and 0.2 μg/ml for LR-MIS (FIG. 4B) for the treated ridge while thecontralateral ridge is left untreated to use as a control. Using thisgold standard bioassay, regression of the Mullerian duct isqualitatively measured and scored on a scale of 0-5, where 0 representsno regression, while 5 is complete regression. Using this bioassay, theinventors tested MIS purified from media of CHO93 (RF-MIS), and LRF18(LRF-MIS) using anti-flag immunoaffinity, or MIS purified from media ofLR11 (LR-MIS), and B9 (WT-MIS) using anti-MIS 6E11 immunoaffinity. Allfour preparations of WT-MIS, LR-MIS, RF-MIS, and LRF-MIS retain theirability to induce regression of the Mullerian duct at 5 μg/ml and at 3μg/ml, whereas only LR-MIS still displays significant regression at 1μg/ml with a score of 4 (FIG. 4A). Activity of LR-MIS was preserved downto concentrations of 0.5 μg/ml with a score of 4, and still had residualactivity at 0.2 μg/ml with a score of 3 (FIG. 4B).

Accordingly, the inventors demonstrate that that LRF-MIS is able tofully regress the Mullerian duct at 5 μg/ml (35 μM) and 3 μg/ml and showgreater activity than RF-MIS and WT-MIS at these concentrations (FIG.4A). Furthermore LRF-MIS continues to display full regression even atlower doses, down to 2 μg/ml, a dose at which WT-MIS no longer shows anyactivity (data not shown). Accordingly, the presence of the leadersequence (L) in the LRF-MIS recombinant human MIS protein results in adose-dependently decrease the regression of the Mullerian ducts, ascompared to the RF-MIS construct, indicating that this construct has ahigher potency and is more active than the RF-MIS construct.

Example 3

MIS was initially cloned by the inventors. The inventors, together withcolleagues in New Zealand, subsequently observed that MIS and its Type I(MISRI) and Type II (MISRII) receptors are expressed in motor neurons(Wang et al, 2005, PNAS, 2005; 102; 16421-16425) and that MIS directlyenhances survival of motor neurons in vitro. Moreover, it wassubsequently reported that knockout of male specific MIS or its Type IIreceptor reduced the number of male motor neurons, which are normally athird higher than in the female (Wang et al, 2009, PNAS, 106(17);7203-7208).

Accordingly, the inventors demonstrated that the modified MIS asdisclosed herein would have a salutary effect in amyotrophic lateralsclerosis (ALS) mice harboring the SOD1G93A mutation. ALS is aprogressive, degenerative disorder affecting motor neurons in the spinalcord, brain stem, and motor cortex, resulting in muscle atrophy,paralysis, and death by respiratory failure, with survival of less thanfive years (Pasinelli and Brown, 2006). About 25% of familial cases(which are 10% of all ALS) have mutations in the SOD1 gene (Rosen et al,1993) that lead, as in the transgenic mice, to relentless motor neurondegeneration.

Accordingly, the inventors demonstrate that the modified MIS variant asdisclosed herein can be used as a potential ALS treatment, therebyresponding to a compelling unmet need in neurology. ALS is among themost devastating disorders in medicine; it remains uniformly fatal.

The inventors demonstrate in FIG. 6 that the survival data after MIStherapy in ALS mice demonstrated a 15 day benefit, which is distinctlyunusual in this ALS model.

Additionally, the present results as demonstrated herein also isindicate that the neuroprotective action of the MIS variant isbeneficial in ALS will have therapeutic indications in many areasincluding not only neurodegeneration but also head trauma and stroke.Further, the inventors have also demonstrated the feasibility ofintravenous, AAV-mediated delivery of MIS, a strategy that mayultimately be applicable to treating ALS and other MIS-responsivedisorders (e.g. ovarian cancer, excess androgen states and anotherhighly lethal disorder for which potent therapies are conspicuouslylacking).

A mouse MIS (mMIS) cDNA was incorporated into an AAV9 virus construct.After confirmation that plasmids containing the MIS cDNA successfullytransfected HEK 293, the AAV9mMIS vector was delivered as a single IVinjection into C57/B16-tgSOD1G93A mice on postnatal Day 1 (P1) (n=9), P7(n=7), and P28 (n=4). The impact of this intervention on survival wascompared to that in control mice injected with PBS. Typically, theuntreated congenic C57/B16-tgSOD1G93A strains survive to −155 days. Bycontrast, transgenic ALS mice injected at P1 suprizingly had a mean 19day extension of life (FIG. 6). When mice from the 3 injection timeswere combined, the average survival benefit or extension of life was 15days compared to mice injected with PBS (all p values significant). Thisimprovement surpasses that of the only FDA-approved ALS drug (Riluzole)in these ALS mice.

At disease endpoint, AAV9-mMIS genome was detected in both the brain andliver (FIG. 7C). MIS protein protein expressed from AAV9-mMIS wasdetected muscle (Mu), liver (L), brain (B), and spinal cord (SC) byWestern blot analysis using a goat polyclonal antibody to MIS (FIGS. 7Band 7C). Thus, the AAV9-mMIS virus injected by tail vein appears tocross the blood brain barrier (FIG. 7C) as previously shown (Duque etal, 2009; Foust et al, 2009; Zhang et al, 2011).

A critical pathological hallmark of neurodegeneration in ALS in humansand in transgenic mice is an exuberant inflammatory processcharacterized by astrogliosis and microgliosis. Therefore the inventorsassessed whether the apparent benefit of AAV9-mMIS on survivalcorrelated with a blunting or decrease of neuroinflammation. For thispurpose, the inventors used the high copy number mutant SJL/B6 SOD1G93Astrain of mice which succumb die due to ALS by ˜120-125 days. AAV9mMIStreated or untreated mice were sacrificed at 105 days to comparehistological features of motor neurons stained with antibodies tophosphorylated neurofilament H (SMI-32) and choline acetyl transferase(ChAT). Astrocytes were targeted by an antibody to glial fibrillaryacidic protein (GFAP), and microglial cells with anti-CD68 antibody.Spinal cords were fixed after cardiac perfusion, harvested, andsectioned, then immunostained to detect expression the SMI32 marker andproteins of mMIS in the ventral horns of the lumbar spinal cord (usingantibodies to MIS produced or validated in the Donahoe laboratory).Motor neuron loss observed in the PBS injected SOD1G93A (controls, n=3litters) (FIG. 8A) was attenuated in the AAV9 mMIS injected mice (n=3litters) (FIG. 8B) as determined by anti-SMI-32 and anti-MISimmunostaining (FIGS. 8B, 8C and 8D). The virus appears to infect motorneurons in the merged images (FIG. 8D).

In addition, these studies demonstrated that the marked astrocytosis(GFAP) of untreated animals was markedly reduced in the ventral horns ofthree litters treated with AAV9-mMIS (FIG. 9A), as quantified in FIG.9B. Accordingly, the inventors demonstrate that the reduction of GFAPastrogliosis is biologically significant, given that activatedastrocytes are the pathogenic hallmark of post-mortem spinal cords, infamilial and sporadic ALS patients and in transgenic mice(Haidet-Phillips, 2011; Maniatis, 2013).

In further studies, the inventors also injected the AAV9-mMIS and acontrol vector into a large cohort of the congenic C57/B16 SOD1 mutantmice for analysis of both histology at 100 or 115 days (PBS=9; mMIS=10)and survival (155 days for controls) (PBS=15; mMIS=15).

Example 4

Design and Creation of AAV Constructs Expressing the MIS Variants.

In parallel with these studies, the inventors generated the appropriatehuman MIS constructs in order to translate the mouse AAV9-mMIS studiesto human trials. The inventors prepared AAV9 constructs incorporatingmodified human MIS as disclosed herein, with three modifications forcomparison; to increase cleavage of human recombinant MIS, improveprotein production, and create size restricted inserts compatible withexpression in AAV vectors. Three modified human MIS cDNAs were created,first modifying the primary prohormone cleavage site at p426-427,necessary for bioactivity, to a consensus Kex/Furin site, then adding aFlag tag for detection, and, finally, substituting a human serum albuminleader with and without the Flag tag (FIG. 1B). All three humanconstructs, importantly, eliminate internal cleavage products observedafter purification of wild type MIS (Lorenzo et al, 2002; MacLaughlin,unpublished).

Adeno-associated virus (AAV) is a small nonpathogenic andhelper-dependent DNA virus containing a 4.7 kb single-stranded viralgenome. One of the limitations with AAV vector is its small packagingsize. However, the rAAV vector genome can readily be packaged with therelatively small MIS cDNA (1.7 kb). To ensure high level expression ofMIS proteins, the inventors have generated an expression cassette drivenby strong CMV-enhanced chicken β-actin promoter (CB); this includes asynthetic intron that boosts transgene expression further (FIGS. 10A and10B). Shown in FIGS. 10A-10B are the designs of two vector genomesexpressing AAV9-mMIS and AAV-R mMIS, with a modified cleavage site. FIG.10A shows the vector expressing the transgene of interest, native wildtype. Each of these constructs were tested after transfection andselection (neomycin) in CHO cells and scaled for production of the humanMIS protein. More than 250 novel primate AAVs serotypes exist, some ofwhich are the most common AAV serotypes currently used for gene transferapplications in vivo. Among them, systemically delivered rAAV9 standsout for its strong ability to cross vasculature by transcytosis andtransduce the liver, heart, muscle, pancreas, and CNS. To generate highlevels of mMIS or human MIS (hMIS) protein in blood circulation and tomaximize its anti-oncogenic effect on the tumor tissue, the inventorshave elected to package the rAAV-MIS constructs using the capsid fromAAV9. Accordingly, after systemic delivery by systemic injection, thevectors will effectively target the liver, heart, pancreas, muscle, andCNS and achieve high level of mMIS or hMIS protein expression from alltargeted tissues through the ubiquitous CB promoter.

rAAV9-mMIS (mouse) was prepared using methods as disclosed herein andcommonly known to persons of ordinary skill in the art and rAAV9-mMISadministered at day 1 after birth (P1) using the facial vein. The doseis scaled by weight of the recipient mice to 4×10′⁴ rAAV9-mMISgenomes/kg, at a concentration of 4×10⁹/μl. Thus, for a newborn mousethe typical dose is 4×10¹¹ genomes in 100 μl. Control mice were injectedwith empty rAAV9 at the same dose and concentration. The recipientSOD1G93A mutant mice can be obtained from Jackson Labs. The inventorselected to use the congenic SOD1G93A all B6 strain for two reasons.First, mice within this congenic strain should be genotypicallyidentical; avoiding some of the inter-individual variability inherent inusing the more frequently studied hybrid B6SJL SOD1G93A ALS mice. And,second, the ALS trait in the congenic B6 strain is associated with aslightly longer survival (˜160 days) as compared to the SJL-B6 strain(˜135 days). This slightly milder phenotype enhances the likelihood ofdetecting benefit from AAV9-mMIS intervention.

After confirming bioactivity in the organ culture bioassay for Mullerianduct regression (FIG. 4A), the transfected CHO cells were expanded inroller bottles and media purified (Lorenzo et al, 2002) byimmunoaffinity (Ragin et al, 1992) chromatography and sequenceconfirmed. These constructs were cloned and each packaged an rAAVbackbone. The rAAV9hMIS vectors were assessed in the mouse model of ALSexpressing the SOD1G93A mutation.

Design and Testing of AAV9 Viral Vectors of Modified hMIS for In VivoDelivery.

To incorporate 3 different version of modified human MIS gene into rAAVgenome, the same vector genome design and capsid selection was used asfor AAV9-mMIS used since all 3 versions of the AAV9 genomes comprisingmodified hMIS sequences are around 3.3 kb and readily packable into AAV9capsid (FIGS. 11A-11C).

Injection of AAV9 virus in nude mice: The AAV9 hMIS vectors carryingLFR, LR, and RF MIS as well as GFP control were injected i.p. at a3×10¹¹ titer in nude mice of 5-6 weeks of age (5 mice per group). Theserum was collected weekly, and MIS concentration was determined byELISA. The serum concentrations from the three MIS groups rises steadilyduring the first three weeks and reaches a plateau, whereas GFP controlshave no detectable hMIS (FIG. 12). Interestingly, as in CHO cells, theLR-MIS is produced at higher levels than the two other constructs, whichwas confirmed at eight weeks by MIS ELISA (FIG. 12).

At week 3, one million human ovarian cancer cells from the OVCAR5 cellline were xenografted subcutaneously in the flank of these mice injectedwith AAV9 hMIS carrying either LFR, LR, and RF MIS variants, and tumorgrowth was monitored at least twice per week with caliper measurements.Interestingly, only the AAV9-LR-MIS vector significantly inhibited thegrowth of these tumors (FIG. 13), demonstrating that it has increasedpotency compared to the other MIS variants, likely due to increasedcleavage and/or the absence of the FLAG tag, which interferes withactivity in vivo. The levels of MIS protein from AAV9-LR-MIS, asdetected by ELISA, were higher in serum than mice injected with AAV9expressing either LRF or RF and the cleavage was improved from 25 to 80%(Table 5).

TABLE 5 Serum Enzyme levels. ALT and AST are liver enzymes, CK =creatine kinase, a muscle enzyme. ALT AST CK AAV9-LR-MIS 39.2 123.4649.5 AAV9-GFP 32.6 112.0 771.3 Normal range 10-190 10-380 249-1013

Accordingly, the inventors have demonstrated a statistically significantdifference in biological response favoring LR human MIS over RF and LRFin in vivo assays of inhibition of OVCAR5 tumors implanted in nude micefor 39 days, and have demonstrated higher sustained levels of LR-MISafter 60 days (3 weeks preimplantation and 39 days post-tumorimplantation) in the mice which were injected with LR vector.Furthermore, the AAV9-LR-MIS demonstrated with tumor inhibition.Accordingly, LR-MIS was scaled-up for protein production and for use asthe optimal construct for delivery via AAV9 vector.

Additionally, the inventors demonstrate that AAV9-GFP resulted influorescence in vivo of the muscle in the body wall (FIG. 14) and liver(data not shown). Furthermore, the inventors observed good weight gainand no obvious toxicity after 60 days after injection of the AAV9-LR-MISinto mice (data not shown) with an ELISA toxicity screen performed toassay liver enzymes (ALT/AST, n=5), and muscle creatine kinase (CK,n=2). Table 5 indicates that these enzyme levels are within normalrange.

Assessment of AAV9-hMIS In Vivo.

The inventors next ascertained whether human MIS (hMIS) variants canrescue motor neurons and prolong survival in the SOD1G93A ALS mice. Asthe inventors demonstate herein in Example 3 in a proof of principalexperiment, that MIS can be used to treat neurodegenerative diseases,e.g., ALS, as AAV9-mMIS was demonstrated to prolong the survival of ALSmice, increase motor neuron survival in vivo and decrease astrogliosis,and as there is good homology between the mouse and human MIS proteins(74.0% at protein level; 75.6% at DNA level), a AAV9-hMIS construct willlikely similarly be used to treat neurodegenerative diseases, e.g., toprolong the survival of ALS mice, increase motor neuron survival in vivoand decrease astrogliosis. Moreover, the major MIS receptors on mousemotor neurons (Misr2) shares close homology to the human homologues(80.6% at the protein level and 83.2 at the DNA level). To confirm thathMIS can reproduce the neuroprotective effects of mMIS in vivo, hMISconstructs shown in FIG. 11A-11C, in particular AAV9-LR-MIS was assessedafter intrathecal (i.t) and intravenous (i.v) delivery.

As a proof of principal experiment, the inventors have previouslydemonstrated that one can prolong survival in the SOD1G93A transgenicALS mice by intrathecally delivered rAAV9 bearing a microRNA toattenuate expression of SOD1 (Wang, H. et al. Widespread spinal cordtransduction by intrathecally injected rAAV delivers efficacious RNAitherapy for amyotrophic lateral sclerosis. in press (2013).). As alsoreported in that study, IT delivery achieves comparable widespread corddelivery of rAAV-GFP in a small, non-human, adult primate (marmoset)prescreened to be negative for anti-AAV antibodies. As referenced above,other investigators have recently also demonstrated that ITadministration can effectively deliver AAV to the spinal cord (Federici,T. et al. Gene Ther 19, 852-859, (2012); Gray, et al., Gene Ther 20,450-459, (2013); Samaranch, L. et al. Hum Gene Ther 23, 382-389,(2012)). Accordingly, the two vectors (rAAV9-hMIS LR; rAAV9-hMIS LRmutated, FIG. 15) were assessed in the SOD1G93A mouse. These weredelivered intrathecally at 2.4×10¹⁰ viral genomes in 5 μl injected via acatheter into the lumbar intrathecal space at 60 days.

Example 5

LR11 is grown in 5 layer flask with 250 ml of DMEM or in 10 layer flasks(1700 cm²) with 500 ml media supplemented with 10% FFCS, 800 ug/ml ofgeneticin, 2 nM glutamine, 100 U/ml penicillin and 100 ug/mlstreptomycin (Invitrogen) maintained confluent for several months in 5%CO2, at 37 C. Once a week, the media is replaced with a serum-free mediawhich omits FFCS and replaces it with non-essential amino acids (NEAA)and ITS (insulin, transferring, selenium) supplements for 72 h. Themedia is then concentrated 10× using tangential flow osmosis membranes.Using these methods media of 4-5 ug/ml is concentrated to 25-50 ug/ml,and effective purification yield of LR-MIS rises to approximately 30%.

TABLE 6 Purification yield from MIS from various constructs using a newserum-free media purification protocol. WT-MIS RF-MIS LRF-MIS LR-MIS MIS16.821 1.236 2.149 4.866 concentration (μg/ml) at 24 hours Production7.597 0.254 0.430 1.142 (pg/cell/day) Concentration in 1.528 0.223 0.4571.411 serum-free media at 24 h Purification yield 15% 20% 20% 30% (%w/w) Percent cleavage 25% 50% 37% 79% in serum-free media

REFERENCES

Each of the applications and patents cited in this text, as well as eachdocument or reference cited in each of the applications and patents(including during the prosecution of each issued patent; “applicationcited documents”), and each of the PCT and foreign applications orpatents corresponding to and/or claiming priority from any of theseapplications and patents, and each of the documents cited or referencedin each of the application cited documents, are hereby expresslyincorporated herein by reference and may be employed in the practice ofthe invention. More generally, documents or references are cited in thistext, either in a Reference List before the claims, or in the textitself; and, each of these documents or references (“herein citedreferences”), as well as each document or reference cited in each of theherein cited references (including any manufacturer's specifications,instructions, etc.), is hereby expressly incorporated herein byreference. Accordingly, the references are each incorporated herein intheir entirety by reference.

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SEQUENCE LISTING:SEQ ID NO: 1 MIS (560AA) - amino acid sequence (underlined identifiesnative MIS leader sequence)mrdlpltsla lvlsalgall gtealraeep avgtsglifr edldwppgsp qeplclvalggdsngssspl rvvgalsaye qaflgavqra rwgprdlatf gvcntgdrqa alpslrrlgawlrdpggqrl vvlhleevtw eptpslrfqe pppggagppe lallvlypgp gpevtvtraglpgagslcps rdtrylvlav drpagawrgs glaltlqprg edsrlstarl qallfgddhrcftrmtpall llprsepapl pahgqldtvp fppprpsael eesppsadpf letltrlvralrvpparasa prlaldpdal agfpqglvnl sdpaalerll dgeeplllll rptaattgdpaplhdptsap watalarrva aelqaaaael rslpglppat apllarllal cpggpgglgdplrallllka lqglrvewrg rdprgpgra

 rsagataadg pcalrelsvd lraersvlipetyqanncqg vcgwpqsdrn prygnhvvll lkmqvrgaal arppccvpta yagkllislseerisahhvp nmvatecgcrSEQ ID NO: 2 LR (559AA) BOLD indicates albumin leader sequence;UNDERLINED identifies the Modified cleavage sitemkwvtfisll flfssaysrg vfrr raeep avgtsglifr edldwppgsp qeplclvalggdsngssspl rvvgalsaye qaflgavqra rwgprdlatf gvcntgdrqa alpslrrlgawlrdpggqrl vvlhleevtw eptpslrfqe pppggagppe lallvlypgp gpevtvtraglpgaqslcps rdtrylvlav drpagawrgs glaltlqprg edsrlstarl qallfgddhrcftrmtpall llprsepapl pahgqldtvp fppprpsael eesppsadpf letltrlvralrvpparasa prlaldpdal agfpqglvnl sdpaalerll dgeep11111 rptaattgdpaplhdptsap watalarrva aelqaaaael rslpglppat apllarllal cpggpgglgdplrallllka lqglrvewrg rdprgpgra R  rsagataadg pcalrelsvd lraersvlipetyqanncqg vcgwpqsdrn prygnhvvll lkmqvrgaal arppccvpta yagkllislseerisahhvp nmvatecgcrSEQ ID NO: 3 LRF (567AA) Italicized indicates Flag tag (DYKDDDDK(SEQID NO: 8))mkwvtfisll flfssaysrg vfrr raeep avgtsglifr edldwppgsp geplclvalggdsngssspl rvvgalsaye qaflgavqra rwgprdlatf gvcntgdrqa alpslrrlgawlrdpggqrl vvlhleevtw eptpslrfqe pppggagppe lallvlypgp gpevtvtraglpgaqslcps rdtrylvlav drpagawrgs glaltlqprg edsrlstarl qallfgddhrcftrmtpall llprsepapl pahgqldtvp fppprpsael eesppsadpf letltrlvralrvpparasa prlaldpdal agfpqglvnl sdpaalerll dgeeplllll rptaattgdpaplhdptsap watalarrva aelqaaaael rslpglppat apllarllal cpggpgglgdplrallllka lqglrvewrg rdprgpgra R  rs

 agataadg   pcalrelsvdlraersvlip etyqanncqg vcgwpqsdrn prygnhvvll lkmqvrgaal arppccvptayagkllisls eerisahhvp nmvatecgcr SEQ ID NO: 4 LR - nucleic acid sequence

GAGGAGCCAGCTGTGGGCACCAGTGGCCTCATCTTCCGAGAAGACTTGGACTGGCCTCCAGGCAGCCCACAAGAGCCTCTGTGCCTGGTGGCACTGGGCGGGGACAGCAATGGCAGCAGCTCCCCCCTGCGGGTGGTGGGGGCTCTAAGCGCCTATGAGCAGGCCTTCCTGGGGGCCGTGCAGAGGGCCCGCTGGGGCCCCCGAGACCTGGCCACCTTCGGGGTCTGCAACACCGGTGACAGGCAGGCTGCCTTGCCCTCTCTACGGCGGCTGGGGGCCTGGCTGCGGGACCCTGGGGGGCAGCGCCTGGTGGTCCTACACCTGGAGGAAGTGACCTGGGAGCCAACACCCTCGCTGAGGTTCCAGGAGCCCCCGCCTGGAGGAGCTGGCCCCCCAGAGCTGGCGCTGCTGGTGCTGTACCCTGGGCCTGGCCCTGAGGTCACTGTGACGAGGGCTGGGCTGCCGGGTGCCCAGAGCCTCTGCCCCTCCCGAGACACCCGCTACCTGGTGTTAGCGGTGGACCGCCCTGCGGGGGCCTGGCGCGGCTCCGGGCTGGCCTTGACCCTGCAGCCCCGCGGAGAGGACTCCCGGCTGAGTACCGCCCGGCTGCAGGCACTGCTGTTCGGCGACGACCACCGCTGCTTCACACGGATGACCCCGGCCCTGCTCCTGCTGCCGCGGTCCGAGCCCGCGCCGCTGCCTGCGCACGGCCAGCTGGACACCGTGCCCTTCCCGCCGCCCAGGCCATCCGCGGAACTCGAGGAGTCGCCACCCAGCGCAGACCCCTTCCTGGAGACGCTCACGCGCCTGGTGCGGGCGCTGCGGGTCCCCCCGGCCCGGGCCTCCGCGCCGCGCCTGGCCCTGGATCCGGACGCGCTGGCCGGCTTCCCGCAGGGCCTAGTCAACCTGTCGGACCCCGCGGCGCTGGAGCGCCTACTCGACGGCGAGGAGCCGCTGCTGCTGCTGCTGAGGCCCACTGCGGCCACCACCGGGGATCCTGCGCCCCTGCACGACCCCACGTCGGCGCCGTGGGCCACGGCCCTGGCGCGCCGCGTGGCTGCTGAACTGCAAGCGGCGGCTGCCGAGCTGCGAAGCCTCCCGGGTCTGCCTCCGGCCACAGCCCCGCTGCTGGCGCGCCTGCTCGCGCTCTGCCCAGGTGGCCCCGGCGGCCTCGGCGATCCCCTGCGAGCGCTGCTGCTCCTGAAGGCGCTGCAGGGCCTGCGCGTGGAGTGGCGCGGGCGGGATCCGCGCGGGCCGGGTCGGGCAC G GCGCAGCGCGGGGGCCACCGCCGCCGACGGGCCGTGCGCGCTGCGCGAGCTCAGCGTAGACCTCCGCGCCGAGCGCTCCGTACTCATCCCCGAGACCTACCAGGCCAACAATTGCCAGGGCGTGTGCGGCTGGCCTCAGTCCGACCGCAACCCGCGCTACGGCAACCACGTGGTGCTGCTGCTGAAGATGCAGGCCCGTGGGGCCGCCCTGGCGCGCCCACCCTGCTGCGTGCCCACCGCCTACGCGGGCAAGCTGCTCATCAGCCTGTCGGAGGAGCGCATCAGCGCGCACCACGTGCCCAACATGGTGGCCACCGAGTGTGGCTGCCGGTGA SEQ ID NO: 5 LRF - nucleic acid sequenceATGAAGTGGGTGAGCTTCATCAGCCTGCTGTTCCTGTTCAGCAGCGCTTACTCCCGCGGTGTGTTCCGCCGCAGAGCAGAGGAGCCAGCTGTGGGCACCAGTGGCCTCATCTTCCGAGAAGACTTGGACTGGCCTCCAGGCAGCCCACAAGAGCCTCTGTGCCTGGTGGCACTGGGCGGGGACAGCAATGGCAGCAGCTCCCCCCTGCGGGTGGTGGGGGCTCTAAGCGCCTATGAGCAGGCCTTCCTGGGGGCCGTGCAGAGGGCCCGCTGGGGCCCCCGAGACCTGGCCACCTTCGGGGTCTGCAACACCGGTGACAGGCAGGCTGCCTTGCCCTCTCTACGGCGGCTGGGGGCCTGGCTGCGGGACCCTGGGGGGCAGCGCCTGGTGGTCCTACACCTGGAGGAAGTGACCTGGGAGCCAACACCCTCGCTGAGGTTCCAGGAGCCCCCGCCTGGAGGAGCTGGCCCCCCAGAGCTGGCGCTGCTGGTGCTGTACCCTGGGCCTGGCCCTGAGGTCACTGTGACGAGGGCTGGGCTGCCGGGTGCCCAGAGCCTCTGCCCCTCCCGAGACACCCGCTACCTGGTGTTAGCGGTGGACCGCCCTGCGGGGGCCTGGCGCGGCTCCGGGCTGGCCTTGACCCTGCAGCCCCGCGGAGAGGACTCCCGGCTGAGTACCGCCCGGCTGCAGGCACTGCTGTTCGGCGACGACCACCGCTGCTTCACACGGATGACCCCGGCCCTGCTCCTGCTGCCGCGGTCCGAGCCCGCGCCGCTGCCTGCGCACGGCCAGCTGGACACCGTGCCCTTCCCGCCGCCCAGGCCATCCGCGGAACTCGAGGAGTCGCCACCCAGCGCAGACCCCTTCCTGGAGACGCTCACGCGCCTGGTGCGGGCGCTGCGGGTCCCCCCGGCCCGGGCCTCCGCGCCGCGCCTGGCCCTGGATCCGGACGCGCTGGCCGGCTTCCCGCAGGGCCTAGTCAACCTGTCGGACCCCGCGGCGCTGGAGCGCCTACTCGACGGCGAGGAGCCGCTGCTGCTGCTGCTGAGGCCCACTGCGGCCACCACCGGGGATCCTGCGCCCCTGCACGACCCCACGTCGGCGCCGTGGGCCACGGCCCTGGCGCGCCGCGTGGCTGCTGAACTGCAAGCGGCGGCTGCCGAGCTGCGAAGCCTCCCGGGTCTGCCTCCGGCCACAGCCCCGCTGCTGGCGCGCCTGCTCGCGCTCTGCCCAGGTGGCCCCGGCGGCCTCGGCGATCCCCTGCGAGCGCTGCTGCTCCTGAAGGCGCTGCAGGGCCTGCGCGTGGAGTGGCGCGGGCGGGATCCGCGCGGGCCGGGTCGGGCAC G GCGCAGC

GCGGGGGCCACCGCCGCCGACGGGCCGTGCGCGCTGCGCGAGCTCAGCGTAGACCTCCGCGCCGAGCGCTCCGTACTCATCCCCGAGACCTACCAGGCCAACAATTGCCAGGGCGTGTGCGGCTGGCCTCAGTCCGACCGCAACCCGCGCTACGGCAACCACGTGGTGCTGCTGCTGAAGATGCAGGCCCGTGGGGCCGCCCTGGCGCGCCCACCCTGCTGCGTGCCCACCGCCTACGCGGGCAAGCTGCTCATCAGCCTGTCGGAGGAGCGCATCAGCGCGCACCACGTGCCCAACATGGTGGCCACCGAGTGTGGCTGCCGGTGASEQ ID NO: 6 HSA Leader Sequence (amino acid sequence):mkwvtfisll flfssaysrg vfrrSEQ ID NO: 7 - HSA Leader Sequence (nucleic acid sequence):

SEQ ID NO: 8 - FLAG tag (amino acid sequence):

SEQ ID NO: 9 - FLAG tag (nucleic acid sequence):gactacaaggatgacgacgacaag

1. A method for treating a subject with a neurodegenerative disease ordisorder, comprising administering a composition comprising arecombinant Mullerian Inhibiting Substance (MIS) protein, wherein therecombinant MIS protein comprises at least one of: (i) a modification ofamino acid 450 of SEQ ID NO: 1 from Q to R to increase cleavage ascompared to in the absence of such a modification, and (ii) amodification of amino acid 452 of SEQ ID NO: 1 from S to R to increasecleavage as compared to in the absence of such a modification, andwherein the vector does not include a nucleic acid encoding a FLAG tag.2.-4. (canceled)
 5. The method of claim 1, wherein one or more neuronsfrom the subject express the MIS type II receptor or a homologue orfunctional fragment thereof.
 6. (canceled)
 7. The method of claim 1,wherein the neurodegenerative disease is selected from the group of:amyotrophic lateral sclerosis (ALS), progressive bulbar palsy,pseudobulbar palsy; primary lateral sclerosis (PLS); progressivemuscular atrophy; spinal muscular atrophy (SMA, including SMA type I,SMA type II, and SMA type III); Fazio-Londe disease; progressivespinobulbar muscular atrophy; congenital SMA with arthrogryposis orpost-polio syndrome (PPS).
 8. The method of claim 1, wherein theneurodegenerative disease is selected from the group of: Alzheimer'sdisease (AD), Parkinson's disease (PD), Huntington's disease (HD),Friedreich's ataxia, cerebellar ataxia, other brain disorders such asbipolar disorder, epilepsy, schizophrenia, depression, mania, autism,ADHD, brain trauma injuries or stroke. 9.-24. (canceled)
 25. The methodof claim 1, wherein the recombinant MIS protein is encoded by a viralvector or expression vector.
 26. (canceled)
 27. (canceled)
 28. Themethod of claim 25, wherein the viral vector is selected from the groupconsisting of an adenoviral vector, adeno-associated vector (AAV), apoxvirus vector or a lentiviral vector.
 29. (canceled)
 30. The method ofclaim 28, wherein the adeno-associated vector (AAV) is AAV9.
 31. Themethod of claim 25, wherein the vector comprises a nucleic acid sequencethat has at least 95% sequence identity to the nucleic acid sequence ofSEQ ID NO: 4 or SEQ ID NO: 5, wherein the nucleic acid sequence isoperatively linked to a tissue- or cell-type specific promoter. 32.-44.(canceled)
 45. The method of claim 31, wherein the nucleic acid encodesa non-MIS leader sequence in place of the MIS leader sequence of aminoacids 1-25 of SEQ ID NO:
 1. 46. The method of claim 45, wherein thenon-MIS leader sequence comprises at least 10 amino acids of SEQ ID NO:6 or a variant that is at least 80% homologous thereto.
 47. The methodvector of claim 25, wherein the vector comprises a nucleic acid sequenceof nucleotides 79-1680 of SEQ ID NO:
 4. 48. The method of claim 31,wherein the nucleic acid sequence comprises SEQ ID NO:
 4. 49. The methodof claim 1, wherein the recombinant MIS protein comprises the amino acidresidues 25-559 of SEQ ID NO:
 2. 50. The method of claim 45, wherein thenon-MIS leader sequence is selected from any in the group consisting of:a human serum albumin (HSA) leader sequence, an immunoglobulin signalpeptide fused to a tissue-type plasminogen activator propeptide(IgSP-tPA), murine immunoglobulin signal peptide (IgSP), a MPIF-1 signalsequence (MKVSVAALSCLMLVTALGSQA (SEQ ID NO: 15)); a stanniocalcin signalsequence (MLQNSAVLLLLVISASA (SEQ ID NO:16)); an invertase signalsequence (MLLQAFLFLLAGFAAKISA (SEQ ID NO:17)); a yeast mating factoralpha signal sequence (K. lactis killer toxin leader sequence); a hybridsignal sequence (MKWVSFISLLFLFSSAYSRSLEKR, (SEQ ID NO:18)); a HSA/MFα-1hybrid signal sequence (MKWVSFISLLFLFSSAYSRSLDKR (SEQ ID NO:19)); a K.lactis killer/MFα-1 fusion leader sequence (MNIFYIFLFLLSFVQGSLDKR (SEQID NO:20)); an immunoglobulin Ig signal sequence (MGWSCIILFLVATATGVHS(SEQ ID NO:21)); a Fibulin B precursor signal sequence(MERAAPSRRVPLPLLLLGGLALLAAGVDA (SEQ ID NO:22)); a clusterin precursorsignal sequence (MMKTLLLFVGLLLTWESGQVLG (SEQ ID NO: 23)); and theinsulin-like growth factor-binding protein 4 signal sequence(MLPLCLVAALLLAAGPGPSLG (SEQ ID NO:24)).
 51. The method of claim 50,wherein a HSA leader sequence is selected from the group consisting of:MKWVTFISLLFLFSSAYS (SEQ ID NO: 13); MKWVTFISLLFLFSSAYSRGVFRR (SEQ ID NO:6); MKWVSFISLLFLFSSAYS (SEQ ID NO: 14).